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Bactericidal efficacy of selected medicinal plant crude extracts and their fractions against common fish pathogens

The emergence of new diseases and the increased use of antibiotics have led to the development of resistant bacterial strains. Thus, there is greater attention to seek new antibacterial agents from the natural sources for combating fish diseases in the aquaculture industry. The present study evaluat...

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Main Authors: Abdul Razak, Rashidah, Shariff, Mohamed, Md. Yusoff, Fatimah, Ismail, Intan Safinar
Format: Article
Language:English
Published: Penerbit Universiti Kebangsaan Malaysia 2019
Online Access:http://psasir.upm.edu.my/id/eprint/80791/1/PLANT.pdf
http://psasir.upm.edu.my/id/eprint/80791/
http://www.ukm.my/jsm/pdf_files/SM-PDF-48-8-2019/05%20Rashidah%20Abdul%20Razak.pdf
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spelling my.upm.eprints.807912020-10-15T21:59:05Z http://psasir.upm.edu.my/id/eprint/80791/ Bactericidal efficacy of selected medicinal plant crude extracts and their fractions against common fish pathogens Abdul Razak, Rashidah Shariff, Mohamed Md. Yusoff, Fatimah Ismail, Intan Safinar The emergence of new diseases and the increased use of antibiotics have led to the development of resistant bacterial strains. Thus, there is greater attention to seek new antibacterial agents from the natural sources for combating fish diseases in the aquaculture industry. The present study evaluated the bactericidal efficacy of crude methanolic and aqueous extracts from Polygonum chinense, Syzygium polyanthum, Premna foetida, Pimenta dioica, Brucea javanica, Vitex negundo, Alpinia conchigera and Clinacanthus nutans against Vibrio harveyi, Vibrio alginolyticus, Vibrio parahaemolyticus and Aeromonas hydrophila using disc diffusion method. The results showed that methanolic extracts of P. dioica, P. foetida and P. chinense, and aqueous extracts of P. dioica and S. polyanthum showed moderate to strong activity (10.8 to 17.2 mm) against all the tested bacteria. These five potential crude extracts were fractionated using liquid-liquid extraction method to obtain the methanol, dichloromethane and ethyl acetate fractions. Among the fractions, ethyl acetate fraction showed the highest activity against all tested bacteria, with minimum inhibition concentration (MIC) values between 0.625 and 10.000 mg/mL. In addition, the five potential crude extracts had low to moderate toxicity with LC50 >100 µg/ mL using brine shrimp cytotoxicity assays. The results of this study indicated that methanolic extracts of P. chinense and P. foetida that showed high bactericidal activity and low toxicity could be good potentials for use in fish culture. Penerbit Universiti Kebangsaan Malaysia 2019 Article PeerReviewed text en http://psasir.upm.edu.my/id/eprint/80791/1/PLANT.pdf Abdul Razak, Rashidah and Shariff, Mohamed and Md. Yusoff, Fatimah and Ismail, Intan Safinar (2019) Bactericidal efficacy of selected medicinal plant crude extracts and their fractions against common fish pathogens. Sains Malaysiana, 48 (8). pp. 1601-1608. ISSN 0126-6039 http://www.ukm.my/jsm/pdf_files/SM-PDF-48-8-2019/05%20Rashidah%20Abdul%20Razak.pdf 10.17576/jsm-2019-4808-05
institution Universiti Putra Malaysia
building UPM Library
collection Institutional Repository
continent Asia
country Malaysia
content_provider Universiti Putra Malaysia
content_source UPM Institutional Repository
url_provider http://psasir.upm.edu.my/
language English
description The emergence of new diseases and the increased use of antibiotics have led to the development of resistant bacterial strains. Thus, there is greater attention to seek new antibacterial agents from the natural sources for combating fish diseases in the aquaculture industry. The present study evaluated the bactericidal efficacy of crude methanolic and aqueous extracts from Polygonum chinense, Syzygium polyanthum, Premna foetida, Pimenta dioica, Brucea javanica, Vitex negundo, Alpinia conchigera and Clinacanthus nutans against Vibrio harveyi, Vibrio alginolyticus, Vibrio parahaemolyticus and Aeromonas hydrophila using disc diffusion method. The results showed that methanolic extracts of P. dioica, P. foetida and P. chinense, and aqueous extracts of P. dioica and S. polyanthum showed moderate to strong activity (10.8 to 17.2 mm) against all the tested bacteria. These five potential crude extracts were fractionated using liquid-liquid extraction method to obtain the methanol, dichloromethane and ethyl acetate fractions. Among the fractions, ethyl acetate fraction showed the highest activity against all tested bacteria, with minimum inhibition concentration (MIC) values between 0.625 and 10.000 mg/mL. In addition, the five potential crude extracts had low to moderate toxicity with LC50 >100 µg/ mL using brine shrimp cytotoxicity assays. The results of this study indicated that methanolic extracts of P. chinense and P. foetida that showed high bactericidal activity and low toxicity could be good potentials for use in fish culture.
format Article
author Abdul Razak, Rashidah
Shariff, Mohamed
Md. Yusoff, Fatimah
Ismail, Intan Safinar
spellingShingle Abdul Razak, Rashidah
Shariff, Mohamed
Md. Yusoff, Fatimah
Ismail, Intan Safinar
Bactericidal efficacy of selected medicinal plant crude extracts and their fractions against common fish pathogens
author_facet Abdul Razak, Rashidah
Shariff, Mohamed
Md. Yusoff, Fatimah
Ismail, Intan Safinar
author_sort Abdul Razak, Rashidah
title Bactericidal efficacy of selected medicinal plant crude extracts and their fractions against common fish pathogens
title_short Bactericidal efficacy of selected medicinal plant crude extracts and their fractions against common fish pathogens
title_full Bactericidal efficacy of selected medicinal plant crude extracts and their fractions against common fish pathogens
title_fullStr Bactericidal efficacy of selected medicinal plant crude extracts and their fractions against common fish pathogens
title_full_unstemmed Bactericidal efficacy of selected medicinal plant crude extracts and their fractions against common fish pathogens
title_sort bactericidal efficacy of selected medicinal plant crude extracts and their fractions against common fish pathogens
publisher Penerbit Universiti Kebangsaan Malaysia
publishDate 2019
url http://psasir.upm.edu.my/id/eprint/80791/1/PLANT.pdf
http://psasir.upm.edu.my/id/eprint/80791/
http://www.ukm.my/jsm/pdf_files/SM-PDF-48-8-2019/05%20Rashidah%20Abdul%20Razak.pdf
_version_ 1681490822080495616
score 13.211869

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