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Isolation and optimisation of phenol degradation by antarctic isolate using one factor at time

Phenol has been classified as a priority pollutant by the United States Environmental Protection Agency (US EPA) due to its high toxicity. The increased level of phenol concentration in the Antarctic environment causes a significant risk to the aquatic and terrestrial lives there due to its persiste...

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Main Authors: Subramanian, Kavilasni, Tengku Marzuki, Tengku Athirrah, Abd. Shukor, Mohd. Yunus, Ahmad, Siti Aqlima
Format: Article
Language:English
Published: Malaysian Society for Biochemistry and Molecular Biology 2019
Online Access:http://psasir.upm.edu.my/id/eprint/80335/1/Isolation%20and%20optimisation%20of%20phenol%20degradation%20by%20antarctic%20isolate%20using%20one%20factor%20at%20time.pdf
http://psasir.upm.edu.my/id/eprint/80335/
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spelling my.upm.eprints.803352020-10-21T19:38:44Z http://psasir.upm.edu.my/id/eprint/80335/ Isolation and optimisation of phenol degradation by antarctic isolate using one factor at time Subramanian, Kavilasni Tengku Marzuki, Tengku Athirrah Abd. Shukor, Mohd. Yunus Ahmad, Siti Aqlima Phenol has been classified as a priority pollutant by the United States Environmental Protection Agency (US EPA) due to its high toxicity. The increased level of phenol concentration in the Antarctic environment causes a significant risk to the aquatic and terrestrial lives there due to its persistence, biomagnification and accumulation in the food chain. The biodegradation of phenol in Antarctica is considered highly challenging owing to its harsh and extremely cold climate. Therefore, actions of bioremediation are crucial to overcome this problem. To date, little data is available regarding the biodegradation of phenol by aboriginal Antarctic bacteria, and reports on the utilisation of phenol as sole carbon source by strains isolated from Antarctic soil are scarce. In the present study, bacteria isolated from Antarctic soil and identified as Arthrobacter sp. strain AQ5-15 based on 16S rRNA sequence was screened and optimised through conventional method for efficient phenol degradation. Based on preliminary screening, AQ5-15 strain was capable of completely degrading 0.5 g/L phenol within 108 h at 10°C. A study on the effects of significant factors including nitrogen source and concentration, salinity, pH and temperature was carried out to optimise the conditions for phenol degradation. Finding revealed that this strain is a psychrotolerant with optimum temperature at 20°C and prefer neutral or near-neutral condition for phenol degradation. The basic knowledge obtained from this study will provide the benefits on custody of the Antarctic environment especially in removing phenol and its derivatives at low temperature. Malaysian Society for Biochemistry and Molecular Biology 2019 Article PeerReviewed text en http://psasir.upm.edu.my/id/eprint/80335/1/Isolation%20and%20optimisation%20of%20phenol%20degradation%20by%20antarctic%20isolate%20using%20one%20factor%20at%20time.pdf Subramanian, Kavilasni and Tengku Marzuki, Tengku Athirrah and Abd. Shukor, Mohd. Yunus and Ahmad, Siti Aqlima (2019) Isolation and optimisation of phenol degradation by antarctic isolate using one factor at time. Malaysian Journal of Biochemistry and Molecular Biology, 22 (1). pp. 79-86. ISSN 1511-2616; ESSN: 2600-9005
institution Universiti Putra Malaysia
building UPM Library
collection Institutional Repository
continent Asia
country Malaysia
content_provider Universiti Putra Malaysia
content_source UPM Institutional Repository
url_provider http://psasir.upm.edu.my/
language English
description Phenol has been classified as a priority pollutant by the United States Environmental Protection Agency (US EPA) due to its high toxicity. The increased level of phenol concentration in the Antarctic environment causes a significant risk to the aquatic and terrestrial lives there due to its persistence, biomagnification and accumulation in the food chain. The biodegradation of phenol in Antarctica is considered highly challenging owing to its harsh and extremely cold climate. Therefore, actions of bioremediation are crucial to overcome this problem. To date, little data is available regarding the biodegradation of phenol by aboriginal Antarctic bacteria, and reports on the utilisation of phenol as sole carbon source by strains isolated from Antarctic soil are scarce. In the present study, bacteria isolated from Antarctic soil and identified as Arthrobacter sp. strain AQ5-15 based on 16S rRNA sequence was screened and optimised through conventional method for efficient phenol degradation. Based on preliminary screening, AQ5-15 strain was capable of completely degrading 0.5 g/L phenol within 108 h at 10°C. A study on the effects of significant factors including nitrogen source and concentration, salinity, pH and temperature was carried out to optimise the conditions for phenol degradation. Finding revealed that this strain is a psychrotolerant with optimum temperature at 20°C and prefer neutral or near-neutral condition for phenol degradation. The basic knowledge obtained from this study will provide the benefits on custody of the Antarctic environment especially in removing phenol and its derivatives at low temperature.
format Article
author Subramanian, Kavilasni
Tengku Marzuki, Tengku Athirrah
Abd. Shukor, Mohd. Yunus
Ahmad, Siti Aqlima
spellingShingle Subramanian, Kavilasni
Tengku Marzuki, Tengku Athirrah
Abd. Shukor, Mohd. Yunus
Ahmad, Siti Aqlima
Isolation and optimisation of phenol degradation by antarctic isolate using one factor at time
author_facet Subramanian, Kavilasni
Tengku Marzuki, Tengku Athirrah
Abd. Shukor, Mohd. Yunus
Ahmad, Siti Aqlima
author_sort Subramanian, Kavilasni
title Isolation and optimisation of phenol degradation by antarctic isolate using one factor at time
title_short Isolation and optimisation of phenol degradation by antarctic isolate using one factor at time
title_full Isolation and optimisation of phenol degradation by antarctic isolate using one factor at time
title_fullStr Isolation and optimisation of phenol degradation by antarctic isolate using one factor at time
title_full_unstemmed Isolation and optimisation of phenol degradation by antarctic isolate using one factor at time
title_sort isolation and optimisation of phenol degradation by antarctic isolate using one factor at time
publisher Malaysian Society for Biochemistry and Molecular Biology
publishDate 2019
url http://psasir.upm.edu.my/id/eprint/80335/1/Isolation%20and%20optimisation%20of%20phenol%20degradation%20by%20antarctic%20isolate%20using%20one%20factor%20at%20time.pdf
http://psasir.upm.edu.my/id/eprint/80335/
_version_ 1681490817405943808
score 13.211869

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