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IL-8 as a potential in-vitro severity biomarker for dengue disease

Dengue is a common infection, caused by dengue virus. There are four different dengue serotypes, with different capacity to cause severe dengue infections. Besides, secondary infections with heterologous serotypes, concurrent infections of multiple dengue serotypes may alter the severity of dengue...

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Main Authors: Soo, Kuan Meng, Tham, Chau Ling, Khalid, Bahariah, Basir, Rusliza, Chee, Hui Yee
Format: Article
Language:English
Published: Malaysian Society of Parasitology and Tropical Medicine 2019
Online Access:http://psasir.upm.edu.my/id/eprint/80147/1/IL-8%20as%20a%20potential%20in-vitro%20severity%20biomarker%20for%20dengue%20disease.pdf
http://psasir.upm.edu.my/id/eprint/80147/
https://www.google.com/search?client=firefox-b-d&q=IL-8+as+a+potential+in-vitro+severity+biomarker+for+dengue+disease
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spelling my.upm.eprints.801472020-09-30T04:19:49Z http://psasir.upm.edu.my/id/eprint/80147/ IL-8 as a potential in-vitro severity biomarker for dengue disease Soo, Kuan Meng Tham, Chau Ling Khalid, Bahariah Basir, Rusliza Chee, Hui Yee Dengue is a common infection, caused by dengue virus. There are four different dengue serotypes, with different capacity to cause severe dengue infections. Besides, secondary infections with heterologous serotypes, concurrent infections of multiple dengue serotypes may alter the severity of dengue infection. This study aims to compare the severity of single infection and concurrent infections of different combinations of dengue serotypes in-vitro. Human mast cells (HMC)-1.1 were infected with single and concurrent infections of multiple dengue serotypes. The infected HMC-1.1 supernatant was then added to human umbilical cord vascular endothelial cells (HUVEC) and severity of dengue infections was measured by the percentage of transendothelial electrical resistance (TEER). Levels of IL- 10, CXCL10 and sTRAIL in HMC-1.1 and IL-8, IL-10 and CXCL10 in HUVEC culture supernatants were measured by the ELISA assays. The result showed that the percentage of TEER values were significantly lower in single infections (p< 0.05), compared to concurrent infections on day 2 and 3, indicating that single infection increase endothelial permeability greater than concurrent infections. IL-8 showed moderate correlation with endothelial permeability (r >0.4), indicating that IL-8 may be suitable as an in-vitro severity biomarker. In conclusion, this in-vitro model presented few similarities with regards to the conditions in dengue patients, suggesting that it could serve as a severity model to test for severity and levels of severity biomarkers upon different dengue virus infections. Malaysian Society of Parasitology and Tropical Medicine 2019 Article PeerReviewed text en http://psasir.upm.edu.my/id/eprint/80147/1/IL-8%20as%20a%20potential%20in-vitro%20severity%20biomarker%20for%20dengue%20disease.pdf Soo, Kuan Meng and Tham, Chau Ling and Khalid, Bahariah and Basir, Rusliza and Chee, Hui Yee (2019) IL-8 as a potential in-vitro severity biomarker for dengue disease. Tropical Biomedicine, 36 (4). pp. 1027-1037. ISSN 0127-5720 https://www.google.com/search?client=firefox-b-d&q=IL-8+as+a+potential+in-vitro+severity+biomarker+for+dengue+disease
institution Universiti Putra Malaysia
building UPM Library
collection Institutional Repository
continent Asia
country Malaysia
content_provider Universiti Putra Malaysia
content_source UPM Institutional Repository
url_provider http://psasir.upm.edu.my/
language English
description Dengue is a common infection, caused by dengue virus. There are four different dengue serotypes, with different capacity to cause severe dengue infections. Besides, secondary infections with heterologous serotypes, concurrent infections of multiple dengue serotypes may alter the severity of dengue infection. This study aims to compare the severity of single infection and concurrent infections of different combinations of dengue serotypes in-vitro. Human mast cells (HMC)-1.1 were infected with single and concurrent infections of multiple dengue serotypes. The infected HMC-1.1 supernatant was then added to human umbilical cord vascular endothelial cells (HUVEC) and severity of dengue infections was measured by the percentage of transendothelial electrical resistance (TEER). Levels of IL- 10, CXCL10 and sTRAIL in HMC-1.1 and IL-8, IL-10 and CXCL10 in HUVEC culture supernatants were measured by the ELISA assays. The result showed that the percentage of TEER values were significantly lower in single infections (p< 0.05), compared to concurrent infections on day 2 and 3, indicating that single infection increase endothelial permeability greater than concurrent infections. IL-8 showed moderate correlation with endothelial permeability (r >0.4), indicating that IL-8 may be suitable as an in-vitro severity biomarker. In conclusion, this in-vitro model presented few similarities with regards to the conditions in dengue patients, suggesting that it could serve as a severity model to test for severity and levels of severity biomarkers upon different dengue virus infections.
format Article
author Soo, Kuan Meng
Tham, Chau Ling
Khalid, Bahariah
Basir, Rusliza
Chee, Hui Yee
spellingShingle Soo, Kuan Meng
Tham, Chau Ling
Khalid, Bahariah
Basir, Rusliza
Chee, Hui Yee
IL-8 as a potential in-vitro severity biomarker for dengue disease
author_facet Soo, Kuan Meng
Tham, Chau Ling
Khalid, Bahariah
Basir, Rusliza
Chee, Hui Yee
author_sort Soo, Kuan Meng
title IL-8 as a potential in-vitro severity biomarker for dengue disease
title_short IL-8 as a potential in-vitro severity biomarker for dengue disease
title_full IL-8 as a potential in-vitro severity biomarker for dengue disease
title_fullStr IL-8 as a potential in-vitro severity biomarker for dengue disease
title_full_unstemmed IL-8 as a potential in-vitro severity biomarker for dengue disease
title_sort il-8 as a potential in-vitro severity biomarker for dengue disease
publisher Malaysian Society of Parasitology and Tropical Medicine
publishDate 2019
url http://psasir.upm.edu.my/id/eprint/80147/1/IL-8%20as%20a%20potential%20in-vitro%20severity%20biomarker%20for%20dengue%20disease.pdf
http://psasir.upm.edu.my/id/eprint/80147/
https://www.google.com/search?client=firefox-b-d&q=IL-8+as+a+potential+in-vitro+severity+biomarker+for+dengue+disease
_version_ 1680322365071818752
score 13.211869

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