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Glioblastoma-associated microglia reprogramming is mediated by functional transfer of extracellular miR-21

Gliomas are primary, diffusely infiltrating brain tumors. Microglia are innate immune cells in the CNS and make up a substantial portion of the tumor mass. Glioma cells shape their microenvironment, communicating with and reprogramming surrounding cells, resulting in enhanced angiogenesis, immune su...

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Main Authors: Abels, Erik R., Maas, Sybren L. N., Nieland, Lisa, Wei, Zhiyun, Cheah, Pike See, Tai, Eric, Kolsteeg, Christy-Joy, Dusoswa, Sophie A., Ting, David T., Hickman, Suzanne, El Khoury, Joseph, Krichevsky, Anna M., Broekman, Marike L. D., Breakefield, Xandra O.
Format: Article
Published: Cell Press 2019
Online Access:http://psasir.upm.edu.my/id/eprint/79998/
https://www.sciencedirect.com/science/article/pii/S2211124719310769?via%3Dihub
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spelling my.upm.eprints.799982023-05-30T08:42:51Z http://psasir.upm.edu.my/id/eprint/79998/ Glioblastoma-associated microglia reprogramming is mediated by functional transfer of extracellular miR-21 Abels, Erik R. Maas, Sybren L. N. Nieland, Lisa Wei, Zhiyun Cheah, Pike See Tai, Eric Kolsteeg, Christy-Joy Dusoswa, Sophie A. Ting, David T. Hickman, Suzanne El Khoury, Joseph Krichevsky, Anna M. Broekman, Marike L. D. Breakefield, Xandra O. Gliomas are primary, diffusely infiltrating brain tumors. Microglia are innate immune cells in the CNS and make up a substantial portion of the tumor mass. Glioma cells shape their microenvironment, communicating with and reprogramming surrounding cells, resulting in enhanced angiogenesis, immune suppression, and remodeling of the extracellular matrix. Glioma cells communicate with microglia, in part by releasing extracellular vesicles (EVs). Mouse glioma cells stably expressing a palmitoylated GFP to label EVs were implanted intracranially into syngeneic miR-21-null mice. Here, we demonstrate functional delivery of miR-21, regulating specific downstream mRNA targets in microglia after uptake of tumor-derived EVs. These findings attest to EV-dependent microRNA delivery as studied in an in vivo-based model and provide insight into the reprograming of microglial cells by tumor cells to create a favorable microenvironment for cancer progression. Cell Press 2019 Article PeerReviewed Abels, Erik R. and Maas, Sybren L. N. and Nieland, Lisa and Wei, Zhiyun and Cheah, Pike See and Tai, Eric and Kolsteeg, Christy-Joy and Dusoswa, Sophie A. and Ting, David T. and Hickman, Suzanne and El Khoury, Joseph and Krichevsky, Anna M. and Broekman, Marike L. D. and Breakefield, Xandra O. (2019) Glioblastoma-associated microglia reprogramming is mediated by functional transfer of extracellular miR-21. Cell Reports, 28 (12). pp. 3105-3119. ISSN 2211-1247 https://www.sciencedirect.com/science/article/pii/S2211124719310769?via%3Dihub 10.1016/j.celrep.2019.08.036
institution Universiti Putra Malaysia
building UPM Library
collection Institutional Repository
continent Asia
country Malaysia
content_provider Universiti Putra Malaysia
content_source UPM Institutional Repository
url_provider http://psasir.upm.edu.my/
description Gliomas are primary, diffusely infiltrating brain tumors. Microglia are innate immune cells in the CNS and make up a substantial portion of the tumor mass. Glioma cells shape their microenvironment, communicating with and reprogramming surrounding cells, resulting in enhanced angiogenesis, immune suppression, and remodeling of the extracellular matrix. Glioma cells communicate with microglia, in part by releasing extracellular vesicles (EVs). Mouse glioma cells stably expressing a palmitoylated GFP to label EVs were implanted intracranially into syngeneic miR-21-null mice. Here, we demonstrate functional delivery of miR-21, regulating specific downstream mRNA targets in microglia after uptake of tumor-derived EVs. These findings attest to EV-dependent microRNA delivery as studied in an in vivo-based model and provide insight into the reprograming of microglial cells by tumor cells to create a favorable microenvironment for cancer progression.
format Article
author Abels, Erik R.
Maas, Sybren L. N.
Nieland, Lisa
Wei, Zhiyun
Cheah, Pike See
Tai, Eric
Kolsteeg, Christy-Joy
Dusoswa, Sophie A.
Ting, David T.
Hickman, Suzanne
El Khoury, Joseph
Krichevsky, Anna M.
Broekman, Marike L. D.
Breakefield, Xandra O.
spellingShingle Abels, Erik R.
Maas, Sybren L. N.
Nieland, Lisa
Wei, Zhiyun
Cheah, Pike See
Tai, Eric
Kolsteeg, Christy-Joy
Dusoswa, Sophie A.
Ting, David T.
Hickman, Suzanne
El Khoury, Joseph
Krichevsky, Anna M.
Broekman, Marike L. D.
Breakefield, Xandra O.
Glioblastoma-associated microglia reprogramming is mediated by functional transfer of extracellular miR-21
author_facet Abels, Erik R.
Maas, Sybren L. N.
Nieland, Lisa
Wei, Zhiyun
Cheah, Pike See
Tai, Eric
Kolsteeg, Christy-Joy
Dusoswa, Sophie A.
Ting, David T.
Hickman, Suzanne
El Khoury, Joseph
Krichevsky, Anna M.
Broekman, Marike L. D.
Breakefield, Xandra O.
author_sort Abels, Erik R.
title Glioblastoma-associated microglia reprogramming is mediated by functional transfer of extracellular miR-21
title_short Glioblastoma-associated microglia reprogramming is mediated by functional transfer of extracellular miR-21
title_full Glioblastoma-associated microglia reprogramming is mediated by functional transfer of extracellular miR-21
title_fullStr Glioblastoma-associated microglia reprogramming is mediated by functional transfer of extracellular miR-21
title_full_unstemmed Glioblastoma-associated microglia reprogramming is mediated by functional transfer of extracellular miR-21
title_sort glioblastoma-associated microglia reprogramming is mediated by functional transfer of extracellular mir-21
publisher Cell Press
publishDate 2019
url http://psasir.upm.edu.my/id/eprint/79998/
https://www.sciencedirect.com/science/article/pii/S2211124719310769?via%3Dihub
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score 13.211869

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