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Factors affecting cell biomass and flavonoid production of Ficus deltoidea var. kunstleri in Cell Suspension Culture System

A study was conducted to establish in vitro culture conditions for maximum production of biomass and flavonoid content for Ficus deltoidea var. kunstleri, locally named as Mas Cotek, known to have a wide variety of potential beneficial attributes for human health. Size of initial inoculum, cell aggr...

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Main Authors: Zainol, Haida, Ahmad, Syahida, Syed Mohd Ariff, Mahmood, Maziah, Awang @ Mansor, Mohd Hakiman
Format: Article
Published: Nature Publishing Group 2019
Online Access:http://psasir.upm.edu.my/id/eprint/79855/
https://www.nature.com/articles/s41598-019-46042-w
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spelling my.upm.eprints.798552022-11-15T09:12:14Z http://psasir.upm.edu.my/id/eprint/79855/ Factors affecting cell biomass and flavonoid production of Ficus deltoidea var. kunstleri in Cell Suspension Culture System Zainol, Haida Ahmad, Syahida Syed Mohd Ariff Mahmood, Maziah Awang @ Mansor, Mohd Hakiman A study was conducted to establish in vitro culture conditions for maximum production of biomass and flavonoid content for Ficus deltoidea var. kunstleri, locally named as Mas Cotek, known to have a wide variety of potential beneficial attributes for human health. Size of initial inoculum, cell aggregate and initial pH value have been suggested to influent content of biomass and flavonoid for cell suspension culture in several plant species. In the present study, leaf explants were cultured by cell suspension culture procedures in MSB5 basal medium supplemented with predetermined supplements of 30 g/L sucrose, 2.75 g/L gelrite, 2 mg/L picloram and 1 mg/L kinetin with continuous agitation of 120 rpm in a standard laboratory environment. Establishment of cell suspension culture was accomplished by culturing resulting callus in different initial fresh weight of cells (0.10, 0.25, 0.50, 1.0, and 2.0 g/25 mL of media) using similar basal medium. The results showed that the highest production of biomass (0.65 g/25 mL of media) was recorded from an initial inoculum size of 2.0 g/25 mL media, whereas the highest flavonoid (3.3 mg RE/g DW) was found in 0.5 g/25 mL of media. Cell suspension fractions classified according to their sizes (500–750 µm, 250–500 µm, and <250 µm). Large cell aggregate size (500–750 µm) cultured at pH 5.75 produced the highest cell biomass (0.28 g/25 mL media) and flavonoid content (3.3 mg RE/g DW). The study had established the optimum conditions for the production of total antioxidant and flavonoid content using DPPH and FRAP assays in cell suspension culture of F. deltoidea var. kunstleri. Nature Publishing Group 2019 Article PeerReviewed Zainol, Haida and Ahmad, Syahida and Syed Mohd Ariff and Mahmood, Maziah and Awang @ Mansor, Mohd Hakiman (2019) Factors affecting cell biomass and flavonoid production of Ficus deltoidea var. kunstleri in Cell Suspension Culture System. Scientific Reports, 9 (1). art. no. 9533. pp. 1-8. ISSN 2045-2322 https://www.nature.com/articles/s41598-019-46042-w 10.1038/s41598-019-46042-w
institution Universiti Putra Malaysia
building UPM Library
collection Institutional Repository
continent Asia
country Malaysia
content_provider Universiti Putra Malaysia
content_source UPM Institutional Repository
url_provider http://psasir.upm.edu.my/
description A study was conducted to establish in vitro culture conditions for maximum production of biomass and flavonoid content for Ficus deltoidea var. kunstleri, locally named as Mas Cotek, known to have a wide variety of potential beneficial attributes for human health. Size of initial inoculum, cell aggregate and initial pH value have been suggested to influent content of biomass and flavonoid for cell suspension culture in several plant species. In the present study, leaf explants were cultured by cell suspension culture procedures in MSB5 basal medium supplemented with predetermined supplements of 30 g/L sucrose, 2.75 g/L gelrite, 2 mg/L picloram and 1 mg/L kinetin with continuous agitation of 120 rpm in a standard laboratory environment. Establishment of cell suspension culture was accomplished by culturing resulting callus in different initial fresh weight of cells (0.10, 0.25, 0.50, 1.0, and 2.0 g/25 mL of media) using similar basal medium. The results showed that the highest production of biomass (0.65 g/25 mL of media) was recorded from an initial inoculum size of 2.0 g/25 mL media, whereas the highest flavonoid (3.3 mg RE/g DW) was found in 0.5 g/25 mL of media. Cell suspension fractions classified according to their sizes (500–750 µm, 250–500 µm, and <250 µm). Large cell aggregate size (500–750 µm) cultured at pH 5.75 produced the highest cell biomass (0.28 g/25 mL media) and flavonoid content (3.3 mg RE/g DW). The study had established the optimum conditions for the production of total antioxidant and flavonoid content using DPPH and FRAP assays in cell suspension culture of F. deltoidea var. kunstleri.
format Article
author Zainol, Haida
Ahmad, Syahida
Syed Mohd Ariff
Mahmood, Maziah
Awang @ Mansor, Mohd Hakiman
spellingShingle Zainol, Haida
Ahmad, Syahida
Syed Mohd Ariff
Mahmood, Maziah
Awang @ Mansor, Mohd Hakiman
Factors affecting cell biomass and flavonoid production of Ficus deltoidea var. kunstleri in Cell Suspension Culture System
author_facet Zainol, Haida
Ahmad, Syahida
Syed Mohd Ariff
Mahmood, Maziah
Awang @ Mansor, Mohd Hakiman
author_sort Zainol, Haida
title Factors affecting cell biomass and flavonoid production of Ficus deltoidea var. kunstleri in Cell Suspension Culture System
title_short Factors affecting cell biomass and flavonoid production of Ficus deltoidea var. kunstleri in Cell Suspension Culture System
title_full Factors affecting cell biomass and flavonoid production of Ficus deltoidea var. kunstleri in Cell Suspension Culture System
title_fullStr Factors affecting cell biomass and flavonoid production of Ficus deltoidea var. kunstleri in Cell Suspension Culture System
title_full_unstemmed Factors affecting cell biomass and flavonoid production of Ficus deltoidea var. kunstleri in Cell Suspension Culture System
title_sort factors affecting cell biomass and flavonoid production of ficus deltoidea var. kunstleri in cell suspension culture system
publisher Nature Publishing Group
publishDate 2019
url http://psasir.upm.edu.my/id/eprint/79855/
https://www.nature.com/articles/s41598-019-46042-w
_version_ 1751538207545098240
score 13.211869

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