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Evaluation of CERS2 gene as a potential biomarker for bladder cancer

The ceramide synthase 2 (CERS2) gene has been linked to tumour recurrence and invasion in many different types of cancers including bladder cancer. In this study, the expression levels of CERS2 in bladder cancer cell lines were analysed using qRT-PCR and the protein expression in clinical bladder ca...

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Main Authors:	Aldoghachi, Ahmed Faris, Baharudin, Aminuddin, Ahmad, Umar, Chan, Soon Choy, Ong, Teng Aik, Yunus, Rosna, Abdul Razack, Azad Hassan, Yusoff, Khatijah, Veerakumarasivam, Abhi
Format:	Article
Published:	Hindawi 2019
Online Access:	http://psasir.upm.edu.my/id/eprint/79746/ https://www.hindawi.com/journals/dm/2019/3875147/
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id	my.upm.eprints.79746
record_format	eprints
spelling	my.upm.eprints.797462022-11-01T03:17:51Z http://psasir.upm.edu.my/id/eprint/79746/ Evaluation of CERS2 gene as a potential biomarker for bladder cancer Aldoghachi, Ahmed Faris Baharudin, Aminuddin Ahmad, Umar Chan, Soon Choy Ong, Teng Aik Yunus, Rosna Abdul Razack, Azad Hassan Yusoff, Khatijah Veerakumarasivam, Abhi The ceramide synthase 2 (CERS2) gene has been linked to tumour recurrence and invasion in many different types of cancers including bladder cancer. In this study, the expression levels of CERS2 in bladder cancer cell lines were analysed using qRT-PCR and the protein expression in clinical bladder cancer histopathological specimens were examined via immunohistochemistry. The potential utility of CERS2 as a predictive biomarker of response to oncolytic virotherapy was assessed by correlating the CERS2 mRNA expression to IC50 values of cells treated with the Newcastle disease virus (NDV), AF2240 strain. This study demonstrates that CERS2 is differentially expressed in different types of bladder cancer cell lines and that the siRNA-mediated downregulation of the expression of CERS2 reduces the migratory potential of UMUC1 bladder cancer cells. However, there were no significant correlations between the expression levels of the CERS2 protein with bladder cancer grade/stage or between the IC50 values of cells treated with NDV and CERS2 expression. Although the utility of CERS2 expression may be limited, its potential as an antimigration cancer therapeutic should be further examined. Hindawi 2019 Article PeerReviewed Aldoghachi, Ahmed Faris and Baharudin, Aminuddin and Ahmad, Umar and Chan, Soon Choy and Ong, Teng Aik and Yunus, Rosna and Abdul Razack, Azad Hassan and Yusoff, Khatijah and Veerakumarasivam, Abhi (2019) Evaluation of CERS2 gene as a potential biomarker for bladder cancer. Disease Markers, 2019. art. no. 3875147. pp. 1-9. ISSN 0278-0240; ESSN: 1875-8630 https://www.hindawi.com/journals/dm/2019/3875147/ 10.1155/2019/3875147
institution	Universiti Putra Malaysia
building	UPM Library
collection	Institutional Repository
continent	Asia
country	Malaysia
content_provider	Universiti Putra Malaysia
content_source	UPM Institutional Repository
url_provider	http://psasir.upm.edu.my/
description	The ceramide synthase 2 (CERS2) gene has been linked to tumour recurrence and invasion in many different types of cancers including bladder cancer. In this study, the expression levels of CERS2 in bladder cancer cell lines were analysed using qRT-PCR and the protein expression in clinical bladder cancer histopathological specimens were examined via immunohistochemistry. The potential utility of CERS2 as a predictive biomarker of response to oncolytic virotherapy was assessed by correlating the CERS2 mRNA expression to IC50 values of cells treated with the Newcastle disease virus (NDV), AF2240 strain. This study demonstrates that CERS2 is differentially expressed in different types of bladder cancer cell lines and that the siRNA-mediated downregulation of the expression of CERS2 reduces the migratory potential of UMUC1 bladder cancer cells. However, there were no significant correlations between the expression levels of the CERS2 protein with bladder cancer grade/stage or between the IC50 values of cells treated with NDV and CERS2 expression. Although the utility of CERS2 expression may be limited, its potential as an antimigration cancer therapeutic should be further examined.
format	Article
author	Aldoghachi, Ahmed Faris Baharudin, Aminuddin Ahmad, Umar Chan, Soon Choy Ong, Teng Aik Yunus, Rosna Abdul Razack, Azad Hassan Yusoff, Khatijah Veerakumarasivam, Abhi
spellingShingle	Aldoghachi, Ahmed Faris Baharudin, Aminuddin Ahmad, Umar Chan, Soon Choy Ong, Teng Aik Yunus, Rosna Abdul Razack, Azad Hassan Yusoff, Khatijah Veerakumarasivam, Abhi Evaluation of CERS2 gene as a potential biomarker for bladder cancer
author_facet	Aldoghachi, Ahmed Faris Baharudin, Aminuddin Ahmad, Umar Chan, Soon Choy Ong, Teng Aik Yunus, Rosna Abdul Razack, Azad Hassan Yusoff, Khatijah Veerakumarasivam, Abhi
author_sort	Aldoghachi, Ahmed Faris
title	Evaluation of CERS2 gene as a potential biomarker for bladder cancer
title_short	Evaluation of CERS2 gene as a potential biomarker for bladder cancer
title_full	Evaluation of CERS2 gene as a potential biomarker for bladder cancer
title_fullStr	Evaluation of CERS2 gene as a potential biomarker for bladder cancer
title_full_unstemmed	Evaluation of CERS2 gene as a potential biomarker for bladder cancer
title_sort	evaluation of cers2 gene as a potential biomarker for bladder cancer
publisher	Hindawi
publishDate	2019
url	http://psasir.upm.edu.my/id/eprint/79746/ https://www.hindawi.com/journals/dm/2019/3875147/
_version_	1748704619181113344
score	13.211869

Evaluation of CERS2 gene as a potential biomarker for bladder cancer

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