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Establishment of chronic Strongyloides ratti infection model and its diagnostic significance

Infection with Strongyloides stercoralis is associated with asymptomatic chronic disease involving the gut and respiratory systems and can persist for long period undetected leading to development of fatal hyperinfection syndrome. The severity is even more in immunocompromised individuals or patien...

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Main Authors: Noor Abdulhaleem, Younis, Hind, Al-Zihiry, Khalid Jameel Kadhim, Awang Hamat, Rukman, Abd Majid, Roslaini, Than, Leslie Thian Lung, Unyah, Ngah Zasmy
Format: Article
Published: Faculty of Medicine, University of Dhi Qar 2019
Online Access:http://psasir.upm.edu.my/id/eprint/79724/
https://www.iasj.net/iasj/article/186908
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spelling my.upm.eprints.797242022-10-27T06:50:15Z http://psasir.upm.edu.my/id/eprint/79724/ Establishment of chronic Strongyloides ratti infection model and its diagnostic significance Noor Abdulhaleem Younis, Hind Al-Zihiry, Khalid Jameel Kadhim Awang Hamat, Rukman Abd Majid, Roslaini Than, Leslie Thian Lung Unyah, Ngah Zasmy Infection with Strongyloides stercoralis is associated with asymptomatic chronic disease involving the gut and respiratory systems and can persist for long period undetected leading to development of fatal hyperinfection syndrome. The severity is even more in immunocompromised individuals or patients undergoing immune suppressive therapy. Unfortunately the disease is difficult to diagnose due to low parasite load and the irregular larval output. Development of efficient diagnostic techniques requires large supply of infective larvae and this can be achieved through in vivo maintenance of the parasite. In this regard, S. ratti is being used as a model instead of S. stercoralis due to difficulty and risk of infection. In this study, a significant amount of previously characterized filariform (iL3) larvae of S. ratti was collected. Chronic infection was successfully established and maintained by experimentally induced immunosuppression of Wistar rats following oral administration of prednisolone. This model can serve as a good source of infective larvae for production of monoclonal antibodies and development of diagnostic assays for prevention of the disease. It can also provide room for further research in diagnostics of human strongyloidiasis. Faculty of Medicine, University of Dhi Qar 2019 Article PeerReviewed Noor Abdulhaleem and Younis, Hind and Al-Zihiry, Khalid Jameel Kadhim and Awang Hamat, Rukman and Abd Majid, Roslaini and Than, Leslie Thian Lung and Unyah, Ngah Zasmy (2019) Establishment of chronic Strongyloides ratti infection model and its diagnostic significance. Thi-Qar Medical Journal, 17 (1). pp. 29-39. ISSN 1992-9218 https://www.iasj.net/iasj/article/186908
institution Universiti Putra Malaysia
building UPM Library
collection Institutional Repository
continent Asia
country Malaysia
content_provider Universiti Putra Malaysia
content_source UPM Institutional Repository
url_provider http://psasir.upm.edu.my/
description Infection with Strongyloides stercoralis is associated with asymptomatic chronic disease involving the gut and respiratory systems and can persist for long period undetected leading to development of fatal hyperinfection syndrome. The severity is even more in immunocompromised individuals or patients undergoing immune suppressive therapy. Unfortunately the disease is difficult to diagnose due to low parasite load and the irregular larval output. Development of efficient diagnostic techniques requires large supply of infective larvae and this can be achieved through in vivo maintenance of the parasite. In this regard, S. ratti is being used as a model instead of S. stercoralis due to difficulty and risk of infection. In this study, a significant amount of previously characterized filariform (iL3) larvae of S. ratti was collected. Chronic infection was successfully established and maintained by experimentally induced immunosuppression of Wistar rats following oral administration of prednisolone. This model can serve as a good source of infective larvae for production of monoclonal antibodies and development of diagnostic assays for prevention of the disease. It can also provide room for further research in diagnostics of human strongyloidiasis.
format Article
author Noor Abdulhaleem
Younis, Hind
Al-Zihiry, Khalid Jameel Kadhim
Awang Hamat, Rukman
Abd Majid, Roslaini
Than, Leslie Thian Lung
Unyah, Ngah Zasmy
spellingShingle Noor Abdulhaleem
Younis, Hind
Al-Zihiry, Khalid Jameel Kadhim
Awang Hamat, Rukman
Abd Majid, Roslaini
Than, Leslie Thian Lung
Unyah, Ngah Zasmy
Establishment of chronic Strongyloides ratti infection model and its diagnostic significance
author_facet Noor Abdulhaleem
Younis, Hind
Al-Zihiry, Khalid Jameel Kadhim
Awang Hamat, Rukman
Abd Majid, Roslaini
Than, Leslie Thian Lung
Unyah, Ngah Zasmy
author_sort Noor Abdulhaleem
title Establishment of chronic Strongyloides ratti infection model and its diagnostic significance
title_short Establishment of chronic Strongyloides ratti infection model and its diagnostic significance
title_full Establishment of chronic Strongyloides ratti infection model and its diagnostic significance
title_fullStr Establishment of chronic Strongyloides ratti infection model and its diagnostic significance
title_full_unstemmed Establishment of chronic Strongyloides ratti infection model and its diagnostic significance
title_sort establishment of chronic strongyloides ratti infection model and its diagnostic significance
publisher Faculty of Medicine, University of Dhi Qar
publishDate 2019
url http://psasir.upm.edu.my/id/eprint/79724/
https://www.iasj.net/iasj/article/186908
_version_ 1748704616721154048
score 13.222552

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