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Enhanced anti-mammary gland cancer activities of tamoxifen-loaded erythropoietin-coated drug delivery system

Nanomedicine is an emerging area in the medical field, particularly in the treatment of cancers. Nanostructured lipid carrier (NLC) was shown to be a good nanoparticulated carrier for the delivery of tamoxifen (TAM). In this study, the tamoxifen-loaded erythropoietin-coated nanostructured lipid carr...

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Main Authors: Beh, Chaw Yee, Rasedee, Abdullah, Selvarajah, Gayathri Thevi, Saiful Yazan, Latifah, Omar, Abdul Rahman, Foong, Jia Ning, How, Chee Wun, Foo, Jhi Biau
Format: Article
Language:English
Published: Public Library of Science 2019
Online Access:http://psasir.upm.edu.my/id/eprint/79681/1/Enhanced%20anti-mammary%20gland%20cancer%20.pdf
http://psasir.upm.edu.my/id/eprint/79681/
https://journals.plos.org/plosone/article?id=10.1371/journal.pone.0219285
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spelling my.upm.eprints.796812022-10-05T01:51:02Z http://psasir.upm.edu.my/id/eprint/79681/ Enhanced anti-mammary gland cancer activities of tamoxifen-loaded erythropoietin-coated drug delivery system Beh, Chaw Yee Rasedee, Abdullah Selvarajah, Gayathri Thevi Saiful Yazan, Latifah Omar, Abdul Rahman Foong, Jia Ning How, Chee Wun Foo, Jhi Biau Nanomedicine is an emerging area in the medical field, particularly in the treatment of cancers. Nanostructured lipid carrier (NLC) was shown to be a good nanoparticulated carrier for the delivery of tamoxifen (TAM). In this study, the tamoxifen-loaded erythropoietin-coated nanostructured lipid carriers (EPO-TAMNLC) were developed to enhance the anti-cancer properties and targetability of TAM, using EPO as the homing ligand for EPO receptors (EpoRs) on breast cancer tissue cells. Tamoxifen-loaded NLC (TAMNLC) was used for comparison. The LA7 cells and LA7 cell-induced rat mammary gland tumor were used as models in the study. Immunocytochemistry staining showed that LA7 cells express estrogen receptors (ERs) and EpoRs. EPO-TAMNLC and TAMNLC significantly (p<0.05) inhibited proliferation of LA7 in dose- and time-dependent manner. EPO-TAMNLC induced apoptosis and G0/G1 cell cycle arrest of LA7 cells. Both drug delivery systems showed anti-mammary gland tumor properties. At an intravenous dose of 5 mg kg-1 body weight, EPO-TAMNLC and TAMNLC were not toxic to rats, suggesting that both are safe therapeutic compounds. In conclusion, EPO-TAMNLC is not only a unique drug delivery system because of the dual drug-loading feature, but also potentially highly specific in the targeting of breast cancer tissues positive for ERs and EpoRs. The incorporation of TAM into NLC with and without EPO coat had significantly (p<0.05) improved specificity and safety of the drug carriers in the treatment of mammary gland tumors. Public Library of Science 2019 Article PeerReviewed text en http://psasir.upm.edu.my/id/eprint/79681/1/Enhanced%20anti-mammary%20gland%20cancer%20.pdf Beh, Chaw Yee and Rasedee, Abdullah and Selvarajah, Gayathri Thevi and Saiful Yazan, Latifah and Omar, Abdul Rahman and Foong, Jia Ning and How, Chee Wun and Foo, Jhi Biau (2019) Enhanced anti-mammary gland cancer activities of tamoxifen-loaded erythropoietin-coated drug delivery system. PLoS One, 14 (7). pp. 1-25. ISSN 1932-6203 https://journals.plos.org/plosone/article?id=10.1371/journal.pone.0219285 10.1371/journal.pone.0219285
institution Universiti Putra Malaysia
building UPM Library
collection Institutional Repository
continent Asia
country Malaysia
content_provider Universiti Putra Malaysia
content_source UPM Institutional Repository
url_provider http://psasir.upm.edu.my/
language English
description Nanomedicine is an emerging area in the medical field, particularly in the treatment of cancers. Nanostructured lipid carrier (NLC) was shown to be a good nanoparticulated carrier for the delivery of tamoxifen (TAM). In this study, the tamoxifen-loaded erythropoietin-coated nanostructured lipid carriers (EPO-TAMNLC) were developed to enhance the anti-cancer properties and targetability of TAM, using EPO as the homing ligand for EPO receptors (EpoRs) on breast cancer tissue cells. Tamoxifen-loaded NLC (TAMNLC) was used for comparison. The LA7 cells and LA7 cell-induced rat mammary gland tumor were used as models in the study. Immunocytochemistry staining showed that LA7 cells express estrogen receptors (ERs) and EpoRs. EPO-TAMNLC and TAMNLC significantly (p<0.05) inhibited proliferation of LA7 in dose- and time-dependent manner. EPO-TAMNLC induced apoptosis and G0/G1 cell cycle arrest of LA7 cells. Both drug delivery systems showed anti-mammary gland tumor properties. At an intravenous dose of 5 mg kg-1 body weight, EPO-TAMNLC and TAMNLC were not toxic to rats, suggesting that both are safe therapeutic compounds. In conclusion, EPO-TAMNLC is not only a unique drug delivery system because of the dual drug-loading feature, but also potentially highly specific in the targeting of breast cancer tissues positive for ERs and EpoRs. The incorporation of TAM into NLC with and without EPO coat had significantly (p<0.05) improved specificity and safety of the drug carriers in the treatment of mammary gland tumors.
format Article
author Beh, Chaw Yee
Rasedee, Abdullah
Selvarajah, Gayathri Thevi
Saiful Yazan, Latifah
Omar, Abdul Rahman
Foong, Jia Ning
How, Chee Wun
Foo, Jhi Biau
spellingShingle Beh, Chaw Yee
Rasedee, Abdullah
Selvarajah, Gayathri Thevi
Saiful Yazan, Latifah
Omar, Abdul Rahman
Foong, Jia Ning
How, Chee Wun
Foo, Jhi Biau
Enhanced anti-mammary gland cancer activities of tamoxifen-loaded erythropoietin-coated drug delivery system
author_facet Beh, Chaw Yee
Rasedee, Abdullah
Selvarajah, Gayathri Thevi
Saiful Yazan, Latifah
Omar, Abdul Rahman
Foong, Jia Ning
How, Chee Wun
Foo, Jhi Biau
author_sort Beh, Chaw Yee
title Enhanced anti-mammary gland cancer activities of tamoxifen-loaded erythropoietin-coated drug delivery system
title_short Enhanced anti-mammary gland cancer activities of tamoxifen-loaded erythropoietin-coated drug delivery system
title_full Enhanced anti-mammary gland cancer activities of tamoxifen-loaded erythropoietin-coated drug delivery system
title_fullStr Enhanced anti-mammary gland cancer activities of tamoxifen-loaded erythropoietin-coated drug delivery system
title_full_unstemmed Enhanced anti-mammary gland cancer activities of tamoxifen-loaded erythropoietin-coated drug delivery system
title_sort enhanced anti-mammary gland cancer activities of tamoxifen-loaded erythropoietin-coated drug delivery system
publisher Public Library of Science
publishDate 2019
url http://psasir.upm.edu.my/id/eprint/79681/1/Enhanced%20anti-mammary%20gland%20cancer%20.pdf
http://psasir.upm.edu.my/id/eprint/79681/
https://journals.plos.org/plosone/article?id=10.1371/journal.pone.0219285
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score 13.211869

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