Isolation of marine bacteria with bioremediation properties from the culture water of Tiger grouper (Epinephelus fuscoguttatus)

An experiment was conducted to isolate bacterial bioremediator from the culture water of Tiger Grouper (Epinephelus fuscoguttatus). In this study, the potential of the bacteria as ammonia degraders were investigated. This was done through enrichment assay using ammonium sulphate as carbon source. Th...

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Main Author: Mohd Amin, Khairuddin
Format: Project Paper Report
Language:English
Published: 2012
Online Access:http://psasir.upm.edu.my/id/eprint/79282/1/FP%202012%20103%20IR.pdf
http://psasir.upm.edu.my/id/eprint/79282/
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spelling my.upm.eprints.792822020-07-07T00:38:46Z http://psasir.upm.edu.my/id/eprint/79282/ Isolation of marine bacteria with bioremediation properties from the culture water of Tiger grouper (Epinephelus fuscoguttatus) Mohd Amin, Khairuddin An experiment was conducted to isolate bacterial bioremediator from the culture water of Tiger Grouper (Epinephelus fuscoguttatus). In this study, the potential of the bacteria as ammonia degraders were investigated. This was done through enrichment assay using ammonium sulphate as carbon source. The bacteria were grown in minimal medium and sodium chloride. Through this assay, three distinct bacteria were isolated. Using the total ammonia-nitrogen (TAN) analysis, all three bacteria were able to degrade ammonium sulphate up to 0.04 ppm compared to the control of 0.54 ppm. All three bacteria were found to be Gram-positive bacteria by its distinctive purple color in Gram-staining method. Based on result, it can be concluded that the marine bacteria isolated from the culture water of Tiger grouper have the potential as bioremediator agent in aquaculture. 2012 Project Paper Report NonPeerReviewed text en http://psasir.upm.edu.my/id/eprint/79282/1/FP%202012%20103%20IR.pdf Mohd Amin, Khairuddin (2012) Isolation of marine bacteria with bioremediation properties from the culture water of Tiger grouper (Epinephelus fuscoguttatus). [Project Paper Report]
institution Universiti Putra Malaysia
building UPM Library
collection Institutional Repository
continent Asia
country Malaysia
content_provider Universiti Putra Malaysia
content_source UPM Institutional Repository
url_provider http://psasir.upm.edu.my/
language English
description An experiment was conducted to isolate bacterial bioremediator from the culture water of Tiger Grouper (Epinephelus fuscoguttatus). In this study, the potential of the bacteria as ammonia degraders were investigated. This was done through enrichment assay using ammonium sulphate as carbon source. The bacteria were grown in minimal medium and sodium chloride. Through this assay, three distinct bacteria were isolated. Using the total ammonia-nitrogen (TAN) analysis, all three bacteria were able to degrade ammonium sulphate up to 0.04 ppm compared to the control of 0.54 ppm. All three bacteria were found to be Gram-positive bacteria by its distinctive purple color in Gram-staining method. Based on result, it can be concluded that the marine bacteria isolated from the culture water of Tiger grouper have the potential as bioremediator agent in aquaculture.
format Project Paper Report
author Mohd Amin, Khairuddin
spellingShingle Mohd Amin, Khairuddin
Isolation of marine bacteria with bioremediation properties from the culture water of Tiger grouper (Epinephelus fuscoguttatus)
author_facet Mohd Amin, Khairuddin
author_sort Mohd Amin, Khairuddin
title Isolation of marine bacteria with bioremediation properties from the culture water of Tiger grouper (Epinephelus fuscoguttatus)
title_short Isolation of marine bacteria with bioremediation properties from the culture water of Tiger grouper (Epinephelus fuscoguttatus)
title_full Isolation of marine bacteria with bioremediation properties from the culture water of Tiger grouper (Epinephelus fuscoguttatus)
title_fullStr Isolation of marine bacteria with bioremediation properties from the culture water of Tiger grouper (Epinephelus fuscoguttatus)
title_full_unstemmed Isolation of marine bacteria with bioremediation properties from the culture water of Tiger grouper (Epinephelus fuscoguttatus)
title_sort isolation of marine bacteria with bioremediation properties from the culture water of tiger grouper (epinephelus fuscoguttatus)
publishDate 2012
url http://psasir.upm.edu.my/id/eprint/79282/1/FP%202012%20103%20IR.pdf
http://psasir.upm.edu.my/id/eprint/79282/
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score 13.211869