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Disease agents affecting the eggs and adults of horseshoe crab, Tachypleus gigas (Muller, 1785) and Carcinoscorpius rotundicauda (Latreille, 1802) in laboratory culture

This study was conducted to identify different disease agents affecting the development of eggs and adults horseshoe crabs and to determine the effect of aeration on the horseshoe crab eggs development. Tachypleus gigas and Carcinoscorpius rotundicauda eggs used in this study were incubated using...

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Main Author: Eng, Heuh Theng
Format: Project Paper Report
Language:English
Published: 2012
Online Access:http://psasir.upm.edu.my/id/eprint/79270/1/FP%202012%2099%20IR.pdf
http://psasir.upm.edu.my/id/eprint/79270/
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spelling my.upm.eprints.792702020-08-05T07:41:53Z http://psasir.upm.edu.my/id/eprint/79270/ Disease agents affecting the eggs and adults of horseshoe crab, Tachypleus gigas (Muller, 1785) and Carcinoscorpius rotundicauda (Latreille, 1802) in laboratory culture Eng, Heuh Theng This study was conducted to identify different disease agents affecting the development of eggs and adults horseshoe crabs and to determine the effect of aeration on the horseshoe crab eggs development. Tachypleus gigas and Carcinoscorpius rotundicauda eggs used in this study were incubated using a conventional culture system. Samples with signs of infection were examined under a compound microscope and photographed. Eggs inoculations were prepared and cultured on Sabouraud Dextrose salt Agar for fungal isolation and TrypticaseTM Soy Broth (TSB) for bacterial isolation. Identification of different disease agents were conducted based on the morphology of fresh and stained mounts as well as the biochemical characters of the isolated fungi and bacteria. Adults of T. gigas and C. rotundicauda were examined similarly. This study was also conducted to determine the effect of aeration on development of T. gigas eggs. Eggs incubations in water medium were carried out in two treatments; with and without aeration. The eggs of T. gigas were infected with Aspergillus sp. and A. niger, while for C. rotundicauda, the eggs were infected with fungi Aspergillus niger, Penicillium sp. and Gliocladium sp. The eggs of T. gigas were infected with bacterial Corynebacterium sp. and Enterococcus faecalis, while C. rotundicauda eggs were infected with E. faecalis and Shewanella putrefaciens. As for adult C. rotundicauda, E. faecalis and Bacillus cereus were isolated from the prosoma and S. putrefaciens was found in the book gills. A trematode worm, identified as Planaria sp., was found in all parts of the body of adult C. rotundicauda. Percentage of hatching was found to be significantly lower (P<0.05) for T. gigas eggs incubated with aeration as compared with those without aeration. 2012 Project Paper Report NonPeerReviewed text en http://psasir.upm.edu.my/id/eprint/79270/1/FP%202012%2099%20IR.pdf Eng, Heuh Theng (2012) Disease agents affecting the eggs and adults of horseshoe crab, Tachypleus gigas (Muller, 1785) and Carcinoscorpius rotundicauda (Latreille, 1802) in laboratory culture. [Project Paper Report]
institution Universiti Putra Malaysia
building UPM Library
collection Institutional Repository
continent Asia
country Malaysia
content_provider Universiti Putra Malaysia
content_source UPM Institutional Repository
url_provider http://psasir.upm.edu.my/
language English
description This study was conducted to identify different disease agents affecting the development of eggs and adults horseshoe crabs and to determine the effect of aeration on the horseshoe crab eggs development. Tachypleus gigas and Carcinoscorpius rotundicauda eggs used in this study were incubated using a conventional culture system. Samples with signs of infection were examined under a compound microscope and photographed. Eggs inoculations were prepared and cultured on Sabouraud Dextrose salt Agar for fungal isolation and TrypticaseTM Soy Broth (TSB) for bacterial isolation. Identification of different disease agents were conducted based on the morphology of fresh and stained mounts as well as the biochemical characters of the isolated fungi and bacteria. Adults of T. gigas and C. rotundicauda were examined similarly. This study was also conducted to determine the effect of aeration on development of T. gigas eggs. Eggs incubations in water medium were carried out in two treatments; with and without aeration. The eggs of T. gigas were infected with Aspergillus sp. and A. niger, while for C. rotundicauda, the eggs were infected with fungi Aspergillus niger, Penicillium sp. and Gliocladium sp. The eggs of T. gigas were infected with bacterial Corynebacterium sp. and Enterococcus faecalis, while C. rotundicauda eggs were infected with E. faecalis and Shewanella putrefaciens. As for adult C. rotundicauda, E. faecalis and Bacillus cereus were isolated from the prosoma and S. putrefaciens was found in the book gills. A trematode worm, identified as Planaria sp., was found in all parts of the body of adult C. rotundicauda. Percentage of hatching was found to be significantly lower (P<0.05) for T. gigas eggs incubated with aeration as compared with those without aeration.
format Project Paper Report
author Eng, Heuh Theng
spellingShingle Eng, Heuh Theng
Disease agents affecting the eggs and adults of horseshoe crab, Tachypleus gigas (Muller, 1785) and Carcinoscorpius rotundicauda (Latreille, 1802) in laboratory culture
author_facet Eng, Heuh Theng
author_sort Eng, Heuh Theng
title Disease agents affecting the eggs and adults of horseshoe crab, Tachypleus gigas (Muller, 1785) and Carcinoscorpius rotundicauda (Latreille, 1802) in laboratory culture
title_short Disease agents affecting the eggs and adults of horseshoe crab, Tachypleus gigas (Muller, 1785) and Carcinoscorpius rotundicauda (Latreille, 1802) in laboratory culture
title_full Disease agents affecting the eggs and adults of horseshoe crab, Tachypleus gigas (Muller, 1785) and Carcinoscorpius rotundicauda (Latreille, 1802) in laboratory culture
title_fullStr Disease agents affecting the eggs and adults of horseshoe crab, Tachypleus gigas (Muller, 1785) and Carcinoscorpius rotundicauda (Latreille, 1802) in laboratory culture
title_full_unstemmed Disease agents affecting the eggs and adults of horseshoe crab, Tachypleus gigas (Muller, 1785) and Carcinoscorpius rotundicauda (Latreille, 1802) in laboratory culture
title_sort disease agents affecting the eggs and adults of horseshoe crab, tachypleus gigas (muller, 1785) and carcinoscorpius rotundicauda (latreille, 1802) in laboratory culture
publishDate 2012
url http://psasir.upm.edu.my/id/eprint/79270/1/FP%202012%2099%20IR.pdf
http://psasir.upm.edu.my/id/eprint/79270/
_version_ 1675328747432050688
score 13.211869

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