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Study on pathogenicity of ORF virus strain UPM 1/14 and 2/14 in mice at different sites of inoculation with and without dexamethasone treatment

Recently, two local ORFV strains (UPM 1/14 Malaysia; UPM 2/14 Malaysia) have been isolated. However, there is no study done in mice using these strains. This study aims to describe the effect of different ORFV strains and inoculation sites as well as dexamethasone effect on pathogenicity of OR...

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Main Author: Tay, Kimmy
Format: Project Paper Report
Language:English
Published: 2016
Online Access:http://psasir.upm.edu.my/id/eprint/78209/1/FPV%202016%2042%20IR.pdf
http://psasir.upm.edu.my/id/eprint/78209/
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spelling my.upm.eprints.782092020-06-04T06:23:56Z http://psasir.upm.edu.my/id/eprint/78209/ Study on pathogenicity of ORF virus strain UPM 1/14 and 2/14 in mice at different sites of inoculation with and without dexamethasone treatment Tay, Kimmy Recently, two local ORFV strains (UPM 1/14 Malaysia; UPM 2/14 Malaysia) have been isolated. However, there is no study done in mice using these strains. This study aims to describe the effect of different ORFV strains and inoculation sites as well as dexamethasone effect on pathogenicity of ORFV in mice. Intradermal injection of 0.2ml 1% UPM 1/14 Malaysia (Group 1) and UPM 2/14 Malaysia (Group 2) were done in each group of 5 mice in Group 1 and Group 2 at dorsum (Group 1A; Group 2A), ear pinna (Group 1B; Group 2B) and labial commissure (Group 1C; Group 2C). Intradermal injection of 0.2ml 1% UPM 1/14 Malaysia was performed in dexamethasone group (n=5) and non-dexamethasone group (n=5). Mice in dexamethasone group were treated with dexamethasone, 5mg/kg/day, intraperitoneally three days prior to challenge and continued until day five post-challenge. In general, mild hyperaemia was observed in all treatment groups. There were no significant difference in mean lesion score among the groups of inoculation site (p>0.05) and between dexamethasone-treated group and non dexamethasone group (p>0.05). Mice in Group 1 and Group 2 showed no significant difference in mean lesion score as well (p>0.05). However, mice in Group 2B and Group 2C had significantly higher mean stratum thickness (p<0.05). Overall histopathological examination revealed keratosis, acanthosis and ballooning degeneration. ORFV was detected by means of PCR on skin tissues of mice with skin lesions. In conclusion, intradermal inoculation of both local strains is able to produce mild skin lesion and histopathological changes in mice. Besides, there is no significant effect of variation in inoculation sites on pathogenicity of ORFV in mice model. In this study, dexamethasone has no statistical effect on pathogenicity of ORFV. Therefore, alternative drug such as cyclosporine can be used for further studies on this aspect. 2016-03 Project Paper Report NonPeerReviewed text en http://psasir.upm.edu.my/id/eprint/78209/1/FPV%202016%2042%20IR.pdf Tay, Kimmy (2016) Study on pathogenicity of ORF virus strain UPM 1/14 and 2/14 in mice at different sites of inoculation with and without dexamethasone treatment. [Project Paper Report]
institution Universiti Putra Malaysia
building UPM Library
collection Institutional Repository
continent Asia
country Malaysia
content_provider Universiti Putra Malaysia
content_source UPM Institutional Repository
url_provider http://psasir.upm.edu.my/
language English
description Recently, two local ORFV strains (UPM 1/14 Malaysia; UPM 2/14 Malaysia) have been isolated. However, there is no study done in mice using these strains. This study aims to describe the effect of different ORFV strains and inoculation sites as well as dexamethasone effect on pathogenicity of ORFV in mice. Intradermal injection of 0.2ml 1% UPM 1/14 Malaysia (Group 1) and UPM 2/14 Malaysia (Group 2) were done in each group of 5 mice in Group 1 and Group 2 at dorsum (Group 1A; Group 2A), ear pinna (Group 1B; Group 2B) and labial commissure (Group 1C; Group 2C). Intradermal injection of 0.2ml 1% UPM 1/14 Malaysia was performed in dexamethasone group (n=5) and non-dexamethasone group (n=5). Mice in dexamethasone group were treated with dexamethasone, 5mg/kg/day, intraperitoneally three days prior to challenge and continued until day five post-challenge. In general, mild hyperaemia was observed in all treatment groups. There were no significant difference in mean lesion score among the groups of inoculation site (p>0.05) and between dexamethasone-treated group and non dexamethasone group (p>0.05). Mice in Group 1 and Group 2 showed no significant difference in mean lesion score as well (p>0.05). However, mice in Group 2B and Group 2C had significantly higher mean stratum thickness (p<0.05). Overall histopathological examination revealed keratosis, acanthosis and ballooning degeneration. ORFV was detected by means of PCR on skin tissues of mice with skin lesions. In conclusion, intradermal inoculation of both local strains is able to produce mild skin lesion and histopathological changes in mice. Besides, there is no significant effect of variation in inoculation sites on pathogenicity of ORFV in mice model. In this study, dexamethasone has no statistical effect on pathogenicity of ORFV. Therefore, alternative drug such as cyclosporine can be used for further studies on this aspect.
format Project Paper Report
author Tay, Kimmy
spellingShingle Tay, Kimmy
Study on pathogenicity of ORF virus strain UPM 1/14 and 2/14 in mice at different sites of inoculation with and without dexamethasone treatment
author_facet Tay, Kimmy
author_sort Tay, Kimmy
title Study on pathogenicity of ORF virus strain UPM 1/14 and 2/14 in mice at different sites of inoculation with and without dexamethasone treatment
title_short Study on pathogenicity of ORF virus strain UPM 1/14 and 2/14 in mice at different sites of inoculation with and without dexamethasone treatment
title_full Study on pathogenicity of ORF virus strain UPM 1/14 and 2/14 in mice at different sites of inoculation with and without dexamethasone treatment
title_fullStr Study on pathogenicity of ORF virus strain UPM 1/14 and 2/14 in mice at different sites of inoculation with and without dexamethasone treatment
title_full_unstemmed Study on pathogenicity of ORF virus strain UPM 1/14 and 2/14 in mice at different sites of inoculation with and without dexamethasone treatment
title_sort study on pathogenicity of orf virus strain upm 1/14 and 2/14 in mice at different sites of inoculation with and without dexamethasone treatment
publishDate 2016
url http://psasir.upm.edu.my/id/eprint/78209/1/FPV%202016%2042%20IR.pdf
http://psasir.upm.edu.my/id/eprint/78209/
_version_ 1669008861007183872
score 13.211869

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