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Optimized aqueous extraction conditions for maximal phenolics, flavonoids and antioxidant capacity from Artocarpus heterophyllus (jackfruit) leaves by response surface methodology (RSM)

Introduction: There are numerous studies on the therapeutic properties of Artocarpus heterophyllus. However, studies on the aqueous extraction of A. heterophyllus leaves are limited. This present study was conducted to optimize the extraction conditions of A. heterophyllus leaves to yield the highes...

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Main Authors: Sayuti, Nor Hafiza, Kamarudin, 'Ammar Akram, Ab Razak, Nor Asma, Saad, Norazalina, Pak Dek, Mohd Sabri, Mohd Esa, Norhaizan
Format: Article
Language:English
Published: Faculty of Medicine and Health Sciences, Universiti Putra Malaysia 2020
Online Access:http://psasir.upm.edu.my/id/eprint/77937/1/2020042010180819_MJMHS_0508.pdf
http://psasir.upm.edu.my/id/eprint/77937/
https://medic.upm.edu.my/upload/dokumen/2020042010180819_MJMHS_0508.pdf
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spelling my.upm.eprints.779372020-05-04T17:47:54Z http://psasir.upm.edu.my/id/eprint/77937/ Optimized aqueous extraction conditions for maximal phenolics, flavonoids and antioxidant capacity from Artocarpus heterophyllus (jackfruit) leaves by response surface methodology (RSM) Sayuti, Nor Hafiza Kamarudin, 'Ammar Akram Ab Razak, Nor Asma Saad, Norazalina Pak Dek, Mohd Sabri Mohd Esa, Norhaizan Introduction: There are numerous studies on the therapeutic properties of Artocarpus heterophyllus. However, studies on the aqueous extraction of A. heterophyllus leaves are limited. This present study was conducted to optimize the extraction conditions of A. heterophyllus leaves to yield the highest phenolic, flavonoids and antioxidant contents. Methods: Response surface methodology (RSM) was employed to obtain a higher phenolic extraction parameter(s) of A. heterophyllus leaves using Central Composite Design (CCD). The antioxidant activity was then determined via ABTS (2,29-azinobis (3 ethylbenzothiazoline-6-sulfonic acid)) and DPPH (2,2-Diphenyl-1-picrylhydrazyl) assay and analysis of the individual phenolics was performed by high performance liquid chromatography (HPLC). Results: The optimum extraction conditions with higher phenolics content and antioxidant activity was achieved at 81°C, 100 min and 40 mL/g sample with a good desirability value of 0.87. Under these optimized parameters, total phenolics and flavonoids were 174.48 ± 4.05 mg GAE/g sample and 21.44 ± 0.05 mg RE/g sample, respectively. Meanwhile, antioxidant activity via ABTS and DPPH assays were 90.88% ± 0.09 and 87.22% ± 0.62, respectively. Finally, under optimal extraction conditions revealed 4 compounds identified as chlorogenic acid, quercetin, rutin and kaempferol. Conclusion: The optimisation are promising to improve phenolic yield and antioxidant activity in A. heterophyllusleaves. It also proved that A. heterophyllus leaves can be used as an alternative natural antioxidant especially in medicinal applications since all identified compound possess significant biological activities for human health. Faculty of Medicine and Health Sciences, Universiti Putra Malaysia 2020 Article PeerReviewed text en http://psasir.upm.edu.my/id/eprint/77937/1/2020042010180819_MJMHS_0508.pdf Sayuti, Nor Hafiza and Kamarudin, 'Ammar Akram and Ab Razak, Nor Asma and Saad, Norazalina and Pak Dek, Mohd Sabri and Mohd Esa, Norhaizan (2020) Optimized aqueous extraction conditions for maximal phenolics, flavonoids and antioxidant capacity from Artocarpus heterophyllus (jackfruit) leaves by response surface methodology (RSM). Malaysian Journal of Medicine and Health Sciences, 16 (2). pp. 135-144. ISSN 1675-8544; ESSN: 2636-9346 https://medic.upm.edu.my/upload/dokumen/2020042010180819_MJMHS_0508.pdf
institution Universiti Putra Malaysia
building UPM Library
collection Institutional Repository
continent Asia
country Malaysia
content_provider Universiti Putra Malaysia
content_source UPM Institutional Repository
url_provider http://psasir.upm.edu.my/
language English
description Introduction: There are numerous studies on the therapeutic properties of Artocarpus heterophyllus. However, studies on the aqueous extraction of A. heterophyllus leaves are limited. This present study was conducted to optimize the extraction conditions of A. heterophyllus leaves to yield the highest phenolic, flavonoids and antioxidant contents. Methods: Response surface methodology (RSM) was employed to obtain a higher phenolic extraction parameter(s) of A. heterophyllus leaves using Central Composite Design (CCD). The antioxidant activity was then determined via ABTS (2,29-azinobis (3 ethylbenzothiazoline-6-sulfonic acid)) and DPPH (2,2-Diphenyl-1-picrylhydrazyl) assay and analysis of the individual phenolics was performed by high performance liquid chromatography (HPLC). Results: The optimum extraction conditions with higher phenolics content and antioxidant activity was achieved at 81°C, 100 min and 40 mL/g sample with a good desirability value of 0.87. Under these optimized parameters, total phenolics and flavonoids were 174.48 ± 4.05 mg GAE/g sample and 21.44 ± 0.05 mg RE/g sample, respectively. Meanwhile, antioxidant activity via ABTS and DPPH assays were 90.88% ± 0.09 and 87.22% ± 0.62, respectively. Finally, under optimal extraction conditions revealed 4 compounds identified as chlorogenic acid, quercetin, rutin and kaempferol. Conclusion: The optimisation are promising to improve phenolic yield and antioxidant activity in A. heterophyllusleaves. It also proved that A. heterophyllus leaves can be used as an alternative natural antioxidant especially in medicinal applications since all identified compound possess significant biological activities for human health.
format Article
author Sayuti, Nor Hafiza
Kamarudin, 'Ammar Akram
Ab Razak, Nor Asma
Saad, Norazalina
Pak Dek, Mohd Sabri
Mohd Esa, Norhaizan
spellingShingle Sayuti, Nor Hafiza
Kamarudin, 'Ammar Akram
Ab Razak, Nor Asma
Saad, Norazalina
Pak Dek, Mohd Sabri
Mohd Esa, Norhaizan
Optimized aqueous extraction conditions for maximal phenolics, flavonoids and antioxidant capacity from Artocarpus heterophyllus (jackfruit) leaves by response surface methodology (RSM)
author_facet Sayuti, Nor Hafiza
Kamarudin, 'Ammar Akram
Ab Razak, Nor Asma
Saad, Norazalina
Pak Dek, Mohd Sabri
Mohd Esa, Norhaizan
author_sort Sayuti, Nor Hafiza
title Optimized aqueous extraction conditions for maximal phenolics, flavonoids and antioxidant capacity from Artocarpus heterophyllus (jackfruit) leaves by response surface methodology (RSM)
title_short Optimized aqueous extraction conditions for maximal phenolics, flavonoids and antioxidant capacity from Artocarpus heterophyllus (jackfruit) leaves by response surface methodology (RSM)
title_full Optimized aqueous extraction conditions for maximal phenolics, flavonoids and antioxidant capacity from Artocarpus heterophyllus (jackfruit) leaves by response surface methodology (RSM)
title_fullStr Optimized aqueous extraction conditions for maximal phenolics, flavonoids and antioxidant capacity from Artocarpus heterophyllus (jackfruit) leaves by response surface methodology (RSM)
title_full_unstemmed Optimized aqueous extraction conditions for maximal phenolics, flavonoids and antioxidant capacity from Artocarpus heterophyllus (jackfruit) leaves by response surface methodology (RSM)
title_sort optimized aqueous extraction conditions for maximal phenolics, flavonoids and antioxidant capacity from artocarpus heterophyllus (jackfruit) leaves by response surface methodology (rsm)
publisher Faculty of Medicine and Health Sciences, Universiti Putra Malaysia
publishDate 2020
url http://psasir.upm.edu.my/id/eprint/77937/1/2020042010180819_MJMHS_0508.pdf
http://psasir.upm.edu.my/id/eprint/77937/
https://medic.upm.edu.my/upload/dokumen/2020042010180819_MJMHS_0508.pdf
_version_ 1665896048110862336
score 13.211869

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