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Enhancement of phenolics and antioxidant activity via heat assisted extraction from Moringa oleifera using response surface methodology and its potential bioactive constituents

Introduction: Moringa oleifera Lam. is a miracle tree that has been widely utilised in folklore medicine due to its immense amount of phenolic constituents that could treat various ailments. Different techniques have been implemented to extract the phenolic but the parameters may not be optimised to...

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Main Authors: Kamarudin, 'Ammar Akram, Saad, Norazalina, Sayuti, Nor Hafiza, Ab Razak, Nor Asma, Mohd Esa, Norhaizan
Format: Article
Language:English
Published: Faculty of Medicine and Health Sciences, Universiti Putra Malaysia 2020
Online Access:http://psasir.upm.edu.my/id/eprint/77934/1/2020042010123212_MJMHS_0498.pdf
http://psasir.upm.edu.my/id/eprint/77934/
https://medic.upm.edu.my/upload/dokumen/2020042010123212_MJMHS_0498.pdf
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spelling my.upm.eprints.779342020-05-04T17:46:57Z http://psasir.upm.edu.my/id/eprint/77934/ Enhancement of phenolics and antioxidant activity via heat assisted extraction from Moringa oleifera using response surface methodology and its potential bioactive constituents Kamarudin, 'Ammar Akram Saad, Norazalina Sayuti, Nor Hafiza Ab Razak, Nor Asma Mohd Esa, Norhaizan Introduction: Moringa oleifera Lam. is a miracle tree that has been widely utilised in folklore medicine due to its immense amount of phenolic constituents that could treat various ailments. Different techniques have been implemented to extract the phenolic but the parameters may not be optimised to further enhance the amount of phenolic extracted. Thus, the work aimed to enhance phenolic content and antioxidant activity of M. oleifera through RSM methodology, which is rapid and convenience. Methods: At first, antioxidant activity of different parts of M. oleifera (leaves, stem, pod and seed) were investigated. The plant part with the highest antioxidant activity was selected for the optimisation of extraction condition using RSM. In RSM, temperature (XA), extraction time (XB) and solid-liquid ratio (XC) were employed to study the effects on yield, total phenolics, flavonoids and antioxidant activity. Then, the optimum extraction condition obtained via RSM was utilised in LC-MS and HPLC analysis to determine the potential bioactive constituents. Results: The leaves of M. oleifera displayed the highest antioxidant activity as compared to other plant parts. The optimum extraction condition obtained for the leaves extract was: temperature (XA): 82°C, extraction time (XB): 48 min and solid-liquid ratio (XC): 1:30 g/mL (w/v). Meanwhile, LC-MS revealed the presence of gallic acid, chlorogenic acid, quercetin, kaempferol and 3-O-glucoside kaempferol. HPLC analysis detected six compounds; gallic acid, epicatechin gallate, chlorogenic acid, myricetin, quercetin and kaempferol. Conclusion: The optimisation are promising to improve yield and antioxidant activity in M. oleifera as compared to non-conventional extractions. Faculty of Medicine and Health Sciences, Universiti Putra Malaysia 2020 Article PeerReviewed text en http://psasir.upm.edu.my/id/eprint/77934/1/2020042010123212_MJMHS_0498.pdf Kamarudin, 'Ammar Akram and Saad, Norazalina and Sayuti, Nor Hafiza and Ab Razak, Nor Asma and Mohd Esa, Norhaizan (2020) Enhancement of phenolics and antioxidant activity via heat assisted extraction from Moringa oleifera using response surface methodology and its potential bioactive constituents. Malaysian Journal of Medicine and Health Sciences, 16 (2). pp. 83-90. ISSN 1675-8544; ESSN: 2636-9346 https://medic.upm.edu.my/upload/dokumen/2020042010123212_MJMHS_0498.pdf
institution Universiti Putra Malaysia
building UPM Library
collection Institutional Repository
continent Asia
country Malaysia
content_provider Universiti Putra Malaysia
content_source UPM Institutional Repository
url_provider http://psasir.upm.edu.my/
language English
description Introduction: Moringa oleifera Lam. is a miracle tree that has been widely utilised in folklore medicine due to its immense amount of phenolic constituents that could treat various ailments. Different techniques have been implemented to extract the phenolic but the parameters may not be optimised to further enhance the amount of phenolic extracted. Thus, the work aimed to enhance phenolic content and antioxidant activity of M. oleifera through RSM methodology, which is rapid and convenience. Methods: At first, antioxidant activity of different parts of M. oleifera (leaves, stem, pod and seed) were investigated. The plant part with the highest antioxidant activity was selected for the optimisation of extraction condition using RSM. In RSM, temperature (XA), extraction time (XB) and solid-liquid ratio (XC) were employed to study the effects on yield, total phenolics, flavonoids and antioxidant activity. Then, the optimum extraction condition obtained via RSM was utilised in LC-MS and HPLC analysis to determine the potential bioactive constituents. Results: The leaves of M. oleifera displayed the highest antioxidant activity as compared to other plant parts. The optimum extraction condition obtained for the leaves extract was: temperature (XA): 82°C, extraction time (XB): 48 min and solid-liquid ratio (XC): 1:30 g/mL (w/v). Meanwhile, LC-MS revealed the presence of gallic acid, chlorogenic acid, quercetin, kaempferol and 3-O-glucoside kaempferol. HPLC analysis detected six compounds; gallic acid, epicatechin gallate, chlorogenic acid, myricetin, quercetin and kaempferol. Conclusion: The optimisation are promising to improve yield and antioxidant activity in M. oleifera as compared to non-conventional extractions.
format Article
author Kamarudin, 'Ammar Akram
Saad, Norazalina
Sayuti, Nor Hafiza
Ab Razak, Nor Asma
Mohd Esa, Norhaizan
spellingShingle Kamarudin, 'Ammar Akram
Saad, Norazalina
Sayuti, Nor Hafiza
Ab Razak, Nor Asma
Mohd Esa, Norhaizan
Enhancement of phenolics and antioxidant activity via heat assisted extraction from Moringa oleifera using response surface methodology and its potential bioactive constituents
author_facet Kamarudin, 'Ammar Akram
Saad, Norazalina
Sayuti, Nor Hafiza
Ab Razak, Nor Asma
Mohd Esa, Norhaizan
author_sort Kamarudin, 'Ammar Akram
title Enhancement of phenolics and antioxidant activity via heat assisted extraction from Moringa oleifera using response surface methodology and its potential bioactive constituents
title_short Enhancement of phenolics and antioxidant activity via heat assisted extraction from Moringa oleifera using response surface methodology and its potential bioactive constituents
title_full Enhancement of phenolics and antioxidant activity via heat assisted extraction from Moringa oleifera using response surface methodology and its potential bioactive constituents
title_fullStr Enhancement of phenolics and antioxidant activity via heat assisted extraction from Moringa oleifera using response surface methodology and its potential bioactive constituents
title_full_unstemmed Enhancement of phenolics and antioxidant activity via heat assisted extraction from Moringa oleifera using response surface methodology and its potential bioactive constituents
title_sort enhancement of phenolics and antioxidant activity via heat assisted extraction from moringa oleifera using response surface methodology and its potential bioactive constituents
publisher Faculty of Medicine and Health Sciences, Universiti Putra Malaysia
publishDate 2020
url http://psasir.upm.edu.my/id/eprint/77934/1/2020042010123212_MJMHS_0498.pdf
http://psasir.upm.edu.my/id/eprint/77934/
https://medic.upm.edu.my/upload/dokumen/2020042010123212_MJMHS_0498.pdf
_version_ 1665896047820406784
score 13.211869

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