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Topical application of Acacia nilotica pods' water extract enhances wound healing in Sprague-Dawley rats by alleviating oxidative stress and suppressing pro-inflammatory cytokines

This study was conducted to validate the folkloric use of Acacia nilotica pods as wound healing agent. Full thickness excision wounds of 6mm diameter were created on Sprague-Dawley rats. Prior to the wound healing assessment, the antioxidant activity of the extract was determined in vitro using 2,2ꞌ...

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Main Authors: Kankara, Sulaiman Sani, Sani, Dahiru, Mustafa, Muskhazli, Ibrahim, Mohd Hafiz, Go, Rusea
Format: Conference or Workshop Item
Language:English
Published: Department of Biology, Faculty of Science, Universiti Putra Malaysia 2016
Online Access:http://psasir.upm.edu.my/id/eprint/77483/1/i-SIMBIOMAS%202016%2025.pdf
http://psasir.upm.edu.my/id/eprint/77483/
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spelling my.upm.eprints.774832020-04-15T16:45:33Z http://psasir.upm.edu.my/id/eprint/77483/ Topical application of Acacia nilotica pods' water extract enhances wound healing in Sprague-Dawley rats by alleviating oxidative stress and suppressing pro-inflammatory cytokines Kankara, Sulaiman Sani Sani, Dahiru Mustafa, Muskhazli Ibrahim, Mohd Hafiz Go, Rusea This study was conducted to validate the folkloric use of Acacia nilotica pods as wound healing agent. Full thickness excision wounds of 6mm diameter were created on Sprague-Dawley rats. Prior to the wound healing assessment, the antioxidant activity of the extract was determined in vitro using 2,2ꞌ- diphenyl-1-picrylhydrazyl (DPPH) radical scavenging activity. Six groups of 6 wounded rats were formed. In each group, the wounds were topically treated with either petroleum jelly (vehicle control), silver sulfadiazine (positive control), 0.5%, 1% or 2% Acacia nilotica cream (treatment groups) prepared by mixing appropriate quantity of A. nilotica pods’ aqueous extract with petroleum jelly. The negative control group received no treatment. The wound healing parameters assessed include wound contraction rate, level of pro-inflammatory cytokines Interleukin1β (IL-1 β) and Tumor Necrosis Factor-α (TNF- α) and histopathological analysis of the wound area. Wounds were assessed on 7th and 14th post wounding days. The extract showed a good, dose dependent DPPH-radical scavenging activity comparable to trolox. Topical application of A. nilotica cream significantly (P< 0.05) enhances wound contraction rate compared to the control groups in both 7th and 14th days of evaluation. The extract also significantly suppressed the expression of both IL-1β and TNF-α in dose dependent manner throughout the study period. The histological analysis revealed that the extract treatment enhanced cellular proliferation as evident by the increased capillary vessels, re-epithelization and dermal tissues regeneration. Overall study suggest that A. nilotica pods’ water extract promotes wound healing in Sprague-Dawly rats by ameliorating oxidative stress and suppression of pro-inflammatory cytokines. This study validates the folkloric use of A. nilotica pods for postpartum wound healing for the first time. Further studies aimed at identifying and isolating active principles from this plant is recommended. Department of Biology, Faculty of Science, Universiti Putra Malaysia 2016 Conference or Workshop Item PeerReviewed text en http://psasir.upm.edu.my/id/eprint/77483/1/i-SIMBIOMAS%202016%2025.pdf Kankara, Sulaiman Sani and Sani, Dahiru and Mustafa, Muskhazli and Ibrahim, Mohd Hafiz and Go, Rusea (2016) Topical application of Acacia nilotica pods' water extract enhances wound healing in Sprague-Dawley rats by alleviating oxidative stress and suppressing pro-inflammatory cytokines. In: Malaysia International Biology Symposium 2016, 26-27 Oct. 2016, PICC, Putrajaya, Malaysia. (p. 25).
institution Universiti Putra Malaysia
building UPM Library
collection Institutional Repository
continent Asia
country Malaysia
content_provider Universiti Putra Malaysia
content_source UPM Institutional Repository
url_provider http://psasir.upm.edu.my/
language English
description This study was conducted to validate the folkloric use of Acacia nilotica pods as wound healing agent. Full thickness excision wounds of 6mm diameter were created on Sprague-Dawley rats. Prior to the wound healing assessment, the antioxidant activity of the extract was determined in vitro using 2,2ꞌ- diphenyl-1-picrylhydrazyl (DPPH) radical scavenging activity. Six groups of 6 wounded rats were formed. In each group, the wounds were topically treated with either petroleum jelly (vehicle control), silver sulfadiazine (positive control), 0.5%, 1% or 2% Acacia nilotica cream (treatment groups) prepared by mixing appropriate quantity of A. nilotica pods’ aqueous extract with petroleum jelly. The negative control group received no treatment. The wound healing parameters assessed include wound contraction rate, level of pro-inflammatory cytokines Interleukin1β (IL-1 β) and Tumor Necrosis Factor-α (TNF- α) and histopathological analysis of the wound area. Wounds were assessed on 7th and 14th post wounding days. The extract showed a good, dose dependent DPPH-radical scavenging activity comparable to trolox. Topical application of A. nilotica cream significantly (P< 0.05) enhances wound contraction rate compared to the control groups in both 7th and 14th days of evaluation. The extract also significantly suppressed the expression of both IL-1β and TNF-α in dose dependent manner throughout the study period. The histological analysis revealed that the extract treatment enhanced cellular proliferation as evident by the increased capillary vessels, re-epithelization and dermal tissues regeneration. Overall study suggest that A. nilotica pods’ water extract promotes wound healing in Sprague-Dawly rats by ameliorating oxidative stress and suppression of pro-inflammatory cytokines. This study validates the folkloric use of A. nilotica pods for postpartum wound healing for the first time. Further studies aimed at identifying and isolating active principles from this plant is recommended.
format Conference or Workshop Item
author Kankara, Sulaiman Sani
Sani, Dahiru
Mustafa, Muskhazli
Ibrahim, Mohd Hafiz
Go, Rusea
spellingShingle Kankara, Sulaiman Sani
Sani, Dahiru
Mustafa, Muskhazli
Ibrahim, Mohd Hafiz
Go, Rusea
Topical application of Acacia nilotica pods' water extract enhances wound healing in Sprague-Dawley rats by alleviating oxidative stress and suppressing pro-inflammatory cytokines
author_facet Kankara, Sulaiman Sani
Sani, Dahiru
Mustafa, Muskhazli
Ibrahim, Mohd Hafiz
Go, Rusea
author_sort Kankara, Sulaiman Sani
title Topical application of Acacia nilotica pods' water extract enhances wound healing in Sprague-Dawley rats by alleviating oxidative stress and suppressing pro-inflammatory cytokines
title_short Topical application of Acacia nilotica pods' water extract enhances wound healing in Sprague-Dawley rats by alleviating oxidative stress and suppressing pro-inflammatory cytokines
title_full Topical application of Acacia nilotica pods' water extract enhances wound healing in Sprague-Dawley rats by alleviating oxidative stress and suppressing pro-inflammatory cytokines
title_fullStr Topical application of Acacia nilotica pods' water extract enhances wound healing in Sprague-Dawley rats by alleviating oxidative stress and suppressing pro-inflammatory cytokines
title_full_unstemmed Topical application of Acacia nilotica pods' water extract enhances wound healing in Sprague-Dawley rats by alleviating oxidative stress and suppressing pro-inflammatory cytokines
title_sort topical application of acacia nilotica pods' water extract enhances wound healing in sprague-dawley rats by alleviating oxidative stress and suppressing pro-inflammatory cytokines
publisher Department of Biology, Faculty of Science, Universiti Putra Malaysia
publishDate 2016
url http://psasir.upm.edu.my/id/eprint/77483/1/i-SIMBIOMAS%202016%2025.pdf
http://psasir.upm.edu.my/id/eprint/77483/
_version_ 1665896028581134336
score 13.211869

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