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Molecular analysis of condensation domain in non-ribosomal peptide synthetase (NRPS) gene of Ganoderma boninense

Non-ribosomal peptides (NRPs) are a class of secondary metabolites produced by bacteria and filamentous fungi. So far, most fungal NRPs have been characterized for ascomycetes and basidiomycetes. Non-ribosomal peptide synthethases (NRPSs) are large multi-modular enzymes which catalyze these compound...

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Main Authors: Shokrollahi, Neda, Ho, Chai Ling, Mohd Zainudin, Nur Ain Izzati, Abdul Wahab, Mohd As'wad, Wong, Mui Yun
Format: Conference or Workshop Item
Language:English
Published: Faculty of Agriculture, Universiti Putra Malaysia 2018
Online Access:http://psasir.upm.edu.my/id/eprint/77380/1/8TH%20IAC%20%E2%80%93%206TH%20ISFA%202018-59.pdf
http://psasir.upm.edu.my/id/eprint/77380/
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spelling my.upm.eprints.773802020-03-05T06:30:03Z http://psasir.upm.edu.my/id/eprint/77380/ Molecular analysis of condensation domain in non-ribosomal peptide synthetase (NRPS) gene of Ganoderma boninense Shokrollahi, Neda Ho, Chai Ling Mohd Zainudin, Nur Ain Izzati Abdul Wahab, Mohd As'wad Wong, Mui Yun Non-ribosomal peptides (NRPs) are a class of secondary metabolites produced by bacteria and filamentous fungi. So far, most fungal NRPs have been characterized for ascomycetes and basidiomycetes. Non-ribosomal peptide synthethases (NRPSs) are large multi-modular enzymes which catalyze these compounds. A minimal module for incorporation of one amino acid into the growing peptide chain consists of an adenylation (A) domain, a thiolation (T) or peptidyl carrier protein (PCP) domain and a condensation (C) domain. The C domain is required for peptide bond formation. The detection of NRPS in Ganoderma boninense and its involvement in basal stem rot (BSR) disease progression in oil palm was detected in an earlier study. However, the precise molecular structure of NRPS remains largely unknown in G. boninense. In this study, we aimed to identify and characterize the NRPS gene in G. boninense strain Gb001 using PCR-based method and bioinformatics tools, ANTISMASH and AUGUSTUS software. The results of this study indicated that a 2000 nucleotides encoding C domain of NRPS gene was detected. BLAST X demonstrated the fragment consisted of AMP- binding and Condensation Super family domains. Further study on differential expression of NRPS transcript on infected oil palm may pave a way towards developing a diagnostic tool for early detection of BSR. Faculty of Agriculture, Universiti Putra Malaysia 2018 Conference or Workshop Item PeerReviewed text en http://psasir.upm.edu.my/id/eprint/77380/1/8TH%20IAC%20%E2%80%93%206TH%20ISFA%202018-59.pdf Shokrollahi, Neda and Ho, Chai Ling and Mohd Zainudin, Nur Ain Izzati and Abdul Wahab, Mohd As'wad and Wong, Mui Yun (2018) Molecular analysis of condensation domain in non-ribosomal peptide synthetase (NRPS) gene of Ganoderma boninense. In: Joint Symposium of the 8th International Agriculture Congress 2018 and 6th International Symposium for Food & Agriculture 2018 (8th IAC – 6th ISFA 2018), 13-15 Nov. 2018, Auditorium Rashdan Baba, TNCPI Building, Universiti Putra Malaysia. (pp. 294-296).
institution Universiti Putra Malaysia
building UPM Library
collection Institutional Repository
continent Asia
country Malaysia
content_provider Universiti Putra Malaysia
content_source UPM Institutional Repository
url_provider http://psasir.upm.edu.my/
language English
description Non-ribosomal peptides (NRPs) are a class of secondary metabolites produced by bacteria and filamentous fungi. So far, most fungal NRPs have been characterized for ascomycetes and basidiomycetes. Non-ribosomal peptide synthethases (NRPSs) are large multi-modular enzymes which catalyze these compounds. A minimal module for incorporation of one amino acid into the growing peptide chain consists of an adenylation (A) domain, a thiolation (T) or peptidyl carrier protein (PCP) domain and a condensation (C) domain. The C domain is required for peptide bond formation. The detection of NRPS in Ganoderma boninense and its involvement in basal stem rot (BSR) disease progression in oil palm was detected in an earlier study. However, the precise molecular structure of NRPS remains largely unknown in G. boninense. In this study, we aimed to identify and characterize the NRPS gene in G. boninense strain Gb001 using PCR-based method and bioinformatics tools, ANTISMASH and AUGUSTUS software. The results of this study indicated that a 2000 nucleotides encoding C domain of NRPS gene was detected. BLAST X demonstrated the fragment consisted of AMP- binding and Condensation Super family domains. Further study on differential expression of NRPS transcript on infected oil palm may pave a way towards developing a diagnostic tool for early detection of BSR.
format Conference or Workshop Item
author Shokrollahi, Neda
Ho, Chai Ling
Mohd Zainudin, Nur Ain Izzati
Abdul Wahab, Mohd As'wad
Wong, Mui Yun
spellingShingle Shokrollahi, Neda
Ho, Chai Ling
Mohd Zainudin, Nur Ain Izzati
Abdul Wahab, Mohd As'wad
Wong, Mui Yun
Molecular analysis of condensation domain in non-ribosomal peptide synthetase (NRPS) gene of Ganoderma boninense
author_facet Shokrollahi, Neda
Ho, Chai Ling
Mohd Zainudin, Nur Ain Izzati
Abdul Wahab, Mohd As'wad
Wong, Mui Yun
author_sort Shokrollahi, Neda
title Molecular analysis of condensation domain in non-ribosomal peptide synthetase (NRPS) gene of Ganoderma boninense
title_short Molecular analysis of condensation domain in non-ribosomal peptide synthetase (NRPS) gene of Ganoderma boninense
title_full Molecular analysis of condensation domain in non-ribosomal peptide synthetase (NRPS) gene of Ganoderma boninense
title_fullStr Molecular analysis of condensation domain in non-ribosomal peptide synthetase (NRPS) gene of Ganoderma boninense
title_full_unstemmed Molecular analysis of condensation domain in non-ribosomal peptide synthetase (NRPS) gene of Ganoderma boninense
title_sort molecular analysis of condensation domain in non-ribosomal peptide synthetase (nrps) gene of ganoderma boninense
publisher Faculty of Agriculture, Universiti Putra Malaysia
publishDate 2018
url http://psasir.upm.edu.my/id/eprint/77380/1/8TH%20IAC%20%E2%80%93%206TH%20ISFA%202018-59.pdf
http://psasir.upm.edu.my/id/eprint/77380/
_version_ 1662756654314160128
score 13.211869

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