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Sequence and transcript analyses of antioxidant genes from Acanthus ebracteatus Vahl.

In this study, we report the sequence, Southern analyses and spatial distribution of four cDNA sequences related to reactive oxygen species (ROS) scavenging systems from a mangrove plant, Acanthus ebracteatus. These four complementary DNA (cDNA) sequences encode cytosolic ascorbate peroxidase (AeAPX...

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Main Authors: Nguyen, Phuoc Dang, Ho, Chai Ling, Teo, Swee Sen, Harikrishna, Jennifer Ann, Abdul Rahim, Raha
Format: Article
Language:English
Published: Springer 2008
Online Access:http://psasir.upm.edu.my/id/eprint/7699/1/Sequence%20and%20transcript%20analyses%20of%20antioxidant%20genes%20from%20Acanthus%20ebracteatus%20Vahl.pdf
http://psasir.upm.edu.my/id/eprint/7699/
https://link.springer.com/article/10.1007%2Fs11295-008-0144-2
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spelling my.upm.eprints.76992019-10-22T02:11:02Z http://psasir.upm.edu.my/id/eprint/7699/ Sequence and transcript analyses of antioxidant genes from Acanthus ebracteatus Vahl. Nguyen, Phuoc Dang Ho, Chai Ling Teo, Swee Sen Harikrishna, Jennifer Ann Abdul Rahim, Raha In this study, we report the sequence, Southern analyses and spatial distribution of four cDNA sequences related to reactive oxygen species (ROS) scavenging systems from a mangrove plant, Acanthus ebracteatus. These four complementary DNA (cDNA) sequences encode cytosolic ascorbate peroxidase (AeAPX), monodehydroascorbate reductase (AeMDHR), glutathione-S-transferase (AeGST), and mitochondrial manganese superoxide dismutase (AeMnSOD), respectively. Experimental results indicated that AeAPX and AeGST belong to multigene families, whereas AeMDHAR and AeMnSOD exhibited substantial differences from other members of the same families. Transcript analyses indicated that all these genes were expressed in flowers. However, only AeAPX, AeMDHAR, and AeMnSOD were expressed in all tissues examined. Although both AeMDHAR and AeMnSOD were highly expressed in flowers, the highest expression of AeMnSOD was in the leaf tissue. The expression of AeMDHAR and AeMnSOD in stem was slightly higher than in the root. AeAPX was expressed at low levels in root and stem tissues and its expression in leaf was slightly higher than in the flower. On the other hand, the transcripts of AeGST were not detected in root and leaf. Its expression was almost equal in stem and flower. The information on spatial distribution and predicted subcellular locations of these antioxidant proteins are important for comprehensive analysis and characterization of ROS scavenging and signaling mechanisms in various plant compartments and tissues. Springer 2008 Article PeerReviewed application/pdf en http://psasir.upm.edu.my/id/eprint/7699/1/Sequence%20and%20transcript%20analyses%20of%20antioxidant%20genes%20from%20Acanthus%20ebracteatus%20Vahl.pdf Nguyen, Phuoc Dang and Ho, Chai Ling and Teo, Swee Sen and Harikrishna, Jennifer Ann and Abdul Rahim, Raha (2008) Sequence and transcript analyses of antioxidant genes from Acanthus ebracteatus Vahl. Tree Genetics & Genomes, 4 (4). pp. 705-713. ISSN 1614-2942; ESSN: 1614-2950 https://link.springer.com/article/10.1007%2Fs11295-008-0144-2 10.1007/s11295-008-0144-2
institution Universiti Putra Malaysia
building UPM Library
collection Institutional Repository
continent Asia
country Malaysia
content_provider Universiti Putra Malaysia
content_source UPM Institutional Repository
url_provider http://psasir.upm.edu.my/
language English
description In this study, we report the sequence, Southern analyses and spatial distribution of four cDNA sequences related to reactive oxygen species (ROS) scavenging systems from a mangrove plant, Acanthus ebracteatus. These four complementary DNA (cDNA) sequences encode cytosolic ascorbate peroxidase (AeAPX), monodehydroascorbate reductase (AeMDHR), glutathione-S-transferase (AeGST), and mitochondrial manganese superoxide dismutase (AeMnSOD), respectively. Experimental results indicated that AeAPX and AeGST belong to multigene families, whereas AeMDHAR and AeMnSOD exhibited substantial differences from other members of the same families. Transcript analyses indicated that all these genes were expressed in flowers. However, only AeAPX, AeMDHAR, and AeMnSOD were expressed in all tissues examined. Although both AeMDHAR and AeMnSOD were highly expressed in flowers, the highest expression of AeMnSOD was in the leaf tissue. The expression of AeMDHAR and AeMnSOD in stem was slightly higher than in the root. AeAPX was expressed at low levels in root and stem tissues and its expression in leaf was slightly higher than in the flower. On the other hand, the transcripts of AeGST were not detected in root and leaf. Its expression was almost equal in stem and flower. The information on spatial distribution and predicted subcellular locations of these antioxidant proteins are important for comprehensive analysis and characterization of ROS scavenging and signaling mechanisms in various plant compartments and tissues.
format Article
author Nguyen, Phuoc Dang
Ho, Chai Ling
Teo, Swee Sen
Harikrishna, Jennifer Ann
Abdul Rahim, Raha
spellingShingle Nguyen, Phuoc Dang
Ho, Chai Ling
Teo, Swee Sen
Harikrishna, Jennifer Ann
Abdul Rahim, Raha
Sequence and transcript analyses of antioxidant genes from Acanthus ebracteatus Vahl.
author_facet Nguyen, Phuoc Dang
Ho, Chai Ling
Teo, Swee Sen
Harikrishna, Jennifer Ann
Abdul Rahim, Raha
author_sort Nguyen, Phuoc Dang
title Sequence and transcript analyses of antioxidant genes from Acanthus ebracteatus Vahl.
title_short Sequence and transcript analyses of antioxidant genes from Acanthus ebracteatus Vahl.
title_full Sequence and transcript analyses of antioxidant genes from Acanthus ebracteatus Vahl.
title_fullStr Sequence and transcript analyses of antioxidant genes from Acanthus ebracteatus Vahl.
title_full_unstemmed Sequence and transcript analyses of antioxidant genes from Acanthus ebracteatus Vahl.
title_sort sequence and transcript analyses of antioxidant genes from acanthus ebracteatus vahl.
publisher Springer
publishDate 2008
url http://psasir.upm.edu.my/id/eprint/7699/1/Sequence%20and%20transcript%20analyses%20of%20antioxidant%20genes%20from%20Acanthus%20ebracteatus%20Vahl.pdf
http://psasir.upm.edu.my/id/eprint/7699/
https://link.springer.com/article/10.1007%2Fs11295-008-0144-2
_version_ 1651869008034529280
score 13.211869

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