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Immunomodulatory effects of betulinic acid isolation from the bark of Melaleuca cajuputi

Betulinic acid and its derivatives showed cytotoxicity against variety of tumour and cancer cell lines comparable to some clinically used drug. In the present study, the immunomodulatory effects of betulinic acid, isolated from the roots of Melaleuca Cajuputi, was studied. Immunomodulatory effect wa...

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Main Authors:	Abd Rahman, Mashitoh, Yeap, Swee Keong, Ali, Abdul Manaf, Ahmad, Faujan, M., Suhaimi, Ng, M. K., Lam, H. Y., Mohamed Alitheen, Noorjahan Banu
Format:	Article
Language:	English
Published:	Universiti Putra Malaysia Press 2012
Online Access:	http://psasir.upm.edu.my/id/eprint/7637/1/Immunomodulatory%20Effects%20of%20Betulinic%20Acid%20Isolation%20from%20the%20Bark%20of%20Melaleuca%20cajuputi%20.pdf http://psasir.upm.edu.my/id/eprint/7637/ http://www.pertanika.upm.edu.my/Pertanika%20PAPERS/JTAS%20Vol.%2035%20(2)%20May.%202012/13%20Pg%20293-305.pdf
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spelling	my.upm.eprints.76372016-01-13T09:14:07Z http://psasir.upm.edu.my/id/eprint/7637/ Immunomodulatory effects of betulinic acid isolation from the bark of Melaleuca cajuputi Abd Rahman, Mashitoh Yeap, Swee Keong Ali, Abdul Manaf Ahmad, Faujan M., Suhaimi Ng, M. K. Lam, H. Y. Mohamed Alitheen, Noorjahan Banu Betulinic acid and its derivatives showed cytotoxicity against variety of tumour and cancer cell lines comparable to some clinically used drug. In the present study, the immunomodulatory effects of betulinic acid, isolated from the roots of Melaleuca Cajuputi, was studied. Immunomodulatory effect was evaluated by using lymphocytes proliferation assay on mice splenocytes, thymocytes and human peripheral blood mononuclear cells (PBMC), while the cell cycle progression of betulinic acid treated PBMC was also studied by using flow cytometer. The production of human interleukin-2 (IL-2) and human inteleukin-12 (IL-12) cytokines was also assessed using enzyme-linked immunosorbent assay (ELISA). The results showed that betulinic acid was able to stimulate the proliferation of mice thymocytes, splenocytes and human PBMC in a time and dose-dependent manner. Meanwhile, betulinic acid treated immune cells were proliferated well at lower concentration (7.5 Âµg /mL), but growth inhibition occurred at a higher concentration (30 Âµg /mL). The findings obtained from the cell cycle analysis exhibited the proliferation effect of betulinic acid on PBMC, whereby 43.66 Â± 2.60% and 42.83 Â± 2.40% of the cells entered G2/M phase after 24h and 48h, respectively. Moreover, betulinic acid also induced extracellular IL-2 and IL-12 production. This finding demonstrates that betulinic acid acts as an immunomodulatory agent that may be useful in enhancing immune system. A. R. Mashitoh, S. K. Yeap, A. M. Ali, A. Faujan, M. Suhaimi, M. K. Ng, H. Y. Lam and N. B. Alitheen Universiti Putra Malaysia Press 2012 Article PeerReviewed application/pdf en http://psasir.upm.edu.my/id/eprint/7637/1/Immunomodulatory%20Effects%20of%20Betulinic%20Acid%20Isolation%20from%20the%20Bark%20of%20Melaleuca%20cajuputi%20.pdf Abd Rahman, Mashitoh and Yeap, Swee Keong and Ali, Abdul Manaf and Ahmad, Faujan and M., Suhaimi and Ng, M. K. and Lam, H. Y. and Mohamed Alitheen, Noorjahan Banu (2012) Immunomodulatory effects of betulinic acid isolation from the bark of Melaleuca cajuputi. Pertanika Journal of Tropical Agricultural Science, 35 (2). pp. 293-305. ISSN 1511-3701; ESSN: 2231-8542 http://www.pertanika.upm.edu.my/Pertanika%20PAPERS/JTAS%20Vol.%2035%20(2)%20May.%202012/13%20Pg%20293-305.pdf
institution	Universiti Putra Malaysia
building	UPM Library
collection	Institutional Repository
continent	Asia
country	Malaysia
content_provider	Universiti Putra Malaysia
content_source	UPM Institutional Repository
url_provider	http://psasir.upm.edu.my/
language	English
description	Betulinic acid and its derivatives showed cytotoxicity against variety of tumour and cancer cell lines comparable to some clinically used drug. In the present study, the immunomodulatory effects of betulinic acid, isolated from the roots of Melaleuca Cajuputi, was studied. Immunomodulatory effect was evaluated by using lymphocytes proliferation assay on mice splenocytes, thymocytes and human peripheral blood mononuclear cells (PBMC), while the cell cycle progression of betulinic acid treated PBMC was also studied by using flow cytometer. The production of human interleukin-2 (IL-2) and human inteleukin-12 (IL-12) cytokines was also assessed using enzyme-linked immunosorbent assay (ELISA). The results showed that betulinic acid was able to stimulate the proliferation of mice thymocytes, splenocytes and human PBMC in a time and dose-dependent manner. Meanwhile, betulinic acid treated immune cells were proliferated well at lower concentration (7.5 Âµg /mL), but growth inhibition occurred at a higher concentration (30 Âµg /mL). The findings obtained from the cell cycle analysis exhibited the proliferation effect of betulinic acid on PBMC, whereby 43.66 Â± 2.60% and 42.83 Â± 2.40% of the cells entered G2/M phase after 24h and 48h, respectively. Moreover, betulinic acid also induced extracellular IL-2 and IL-12 production. This finding demonstrates that betulinic acid acts as an immunomodulatory agent that may be useful in enhancing immune system. A. R. Mashitoh, S. K. Yeap, A. M. Ali, A. Faujan, M. Suhaimi, M. K. Ng, H. Y. Lam and N. B. Alitheen
format	Article
author	Abd Rahman, Mashitoh Yeap, Swee Keong Ali, Abdul Manaf Ahmad, Faujan M., Suhaimi Ng, M. K. Lam, H. Y. Mohamed Alitheen, Noorjahan Banu
spellingShingle	Abd Rahman, Mashitoh Yeap, Swee Keong Ali, Abdul Manaf Ahmad, Faujan M., Suhaimi Ng, M. K. Lam, H. Y. Mohamed Alitheen, Noorjahan Banu Immunomodulatory effects of betulinic acid isolation from the bark of Melaleuca cajuputi
author_facet	Abd Rahman, Mashitoh Yeap, Swee Keong Ali, Abdul Manaf Ahmad, Faujan M., Suhaimi Ng, M. K. Lam, H. Y. Mohamed Alitheen, Noorjahan Banu
author_sort	Abd Rahman, Mashitoh
title	Immunomodulatory effects of betulinic acid isolation from the bark of Melaleuca cajuputi
title_short	Immunomodulatory effects of betulinic acid isolation from the bark of Melaleuca cajuputi
title_full	Immunomodulatory effects of betulinic acid isolation from the bark of Melaleuca cajuputi
title_fullStr	Immunomodulatory effects of betulinic acid isolation from the bark of Melaleuca cajuputi
title_full_unstemmed	Immunomodulatory effects of betulinic acid isolation from the bark of Melaleuca cajuputi
title_sort	immunomodulatory effects of betulinic acid isolation from the bark of melaleuca cajuputi
publisher	Universiti Putra Malaysia Press
publishDate	2012
url	http://psasir.upm.edu.my/id/eprint/7637/1/Immunomodulatory%20Effects%20of%20Betulinic%20Acid%20Isolation%20from%20the%20Bark%20of%20Melaleuca%20cajuputi%20.pdf http://psasir.upm.edu.my/id/eprint/7637/ http://www.pertanika.upm.edu.my/Pertanika%20PAPERS/JTAS%20Vol.%2035%20(2)%20May.%202012/13%20Pg%20293-305.pdf
_version_	1643823782644678656
score	13.211869

Immunomodulatory effects of betulinic acid isolation from the bark of Melaleuca cajuputi

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