Cover Image

Production of novel antimicrobial peptide human beta defensin 9 and its efficacy against common eye pathogenic bacteria in different salt concentration

Beta defensins are small cationic peptide secreted by the surface epithelium in response to microbial threat. The human beta-defensin 9 (hBD9) is a relatively new defensin and its role as antimicrobial agent has not been described previously. In this study, Escherichia coli Mach 1 and E.coli BL21 (D...

Full description

Saved in:
Bibliographic Details
Main Author: Ab Hamid, Nurul Atikah
Format: Thesis
Language:English
Published: 2018
Online Access:http://psasir.upm.edu.my/id/eprint/76290/1/FPSK%28M%29%202018%2025%20-%20IR.pdf
http://psasir.upm.edu.my/id/eprint/76290/
Tags: Add Tag
No Tags, Be the first to tag this record!
id my.upm.eprints.76290
record_format eprints
spelling my.upm.eprints.762902019-12-04T04:03:57Z http://psasir.upm.edu.my/id/eprint/76290/ Production of novel antimicrobial peptide human beta defensin 9 and its efficacy against common eye pathogenic bacteria in different salt concentration Ab Hamid, Nurul Atikah Beta defensins are small cationic peptide secreted by the surface epithelium in response to microbial threat. The human beta-defensin 9 (hBD9) is a relatively new defensin and its role as antimicrobial agent has not been described previously. In this study, Escherichia coli Mach 1 and E.coli BL21 (DE3) were used as cloning and protein expression hosts respectively. HBD9 gene was fused to a protein expression vector using the small ubiquitin-related modifier (SUMO) fusion system. The soluble hBD9-SUMO fusion protein expressed was purified by Ni-IDA immobolised metal affinity chromatography (IMAC) and SUMO was removed by SUMO protease. This study defined the novel antimicrobial peptide hBD9 and its efficacy against common Gram-positive and negative bacteria. Staphylococcus aureus and Pseudomonas aeruginosa were chosen because they are common bacteria that causing eye infection. The Minimal inhibitory concentration (MIC) and Minimal bactericidal concentration (MBC) of the hBD9 were determined for these microbes. Interestingly, hBD9 showed antimicrobial activity against Staphylococcus aureus but not Pseudomonas aeruginosa. Salt sensitivity and cationic concentration were tested by incubation with NaCl and MgCl2 using liquid culture containing hBD9 to analyse the effects of NaCl and MgCl2 concentration on the antimicrobial activity. Antimicrobial activity against Staphylococcus aureus of hBD9 was not supressed by NaCl and MgCl2. All the antimicrobial property of the hBD9 protein against the common bacteria was verified by using conventional methods. The results improved the current knowledge on the hBD9 and its antimicrobial properties. This will drive towards the discovery of a broad spectrum, safe by effective resistant-free antibiotic in the future. The finding of this study can be used as a reference in the future investigation and it might benefits scientific community to better by others scientist or researchers to increase understanding and find new effective antibiotics. 2018-04 Thesis NonPeerReviewed text en http://psasir.upm.edu.my/id/eprint/76290/1/FPSK%28M%29%202018%2025%20-%20IR.pdf Ab Hamid, Nurul Atikah (2018) Production of novel antimicrobial peptide human beta defensin 9 and its efficacy against common eye pathogenic bacteria in different salt concentration. Masters thesis, Universiti Putra Malaysia.
institution Universiti Putra Malaysia
building UPM Library
collection Institutional Repository
continent Asia
country Malaysia
content_provider Universiti Putra Malaysia
content_source UPM Institutional Repository
url_provider http://psasir.upm.edu.my/
language English
description Beta defensins are small cationic peptide secreted by the surface epithelium in response to microbial threat. The human beta-defensin 9 (hBD9) is a relatively new defensin and its role as antimicrobial agent has not been described previously. In this study, Escherichia coli Mach 1 and E.coli BL21 (DE3) were used as cloning and protein expression hosts respectively. HBD9 gene was fused to a protein expression vector using the small ubiquitin-related modifier (SUMO) fusion system. The soluble hBD9-SUMO fusion protein expressed was purified by Ni-IDA immobolised metal affinity chromatography (IMAC) and SUMO was removed by SUMO protease. This study defined the novel antimicrobial peptide hBD9 and its efficacy against common Gram-positive and negative bacteria. Staphylococcus aureus and Pseudomonas aeruginosa were chosen because they are common bacteria that causing eye infection. The Minimal inhibitory concentration (MIC) and Minimal bactericidal concentration (MBC) of the hBD9 were determined for these microbes. Interestingly, hBD9 showed antimicrobial activity against Staphylococcus aureus but not Pseudomonas aeruginosa. Salt sensitivity and cationic concentration were tested by incubation with NaCl and MgCl2 using liquid culture containing hBD9 to analyse the effects of NaCl and MgCl2 concentration on the antimicrobial activity. Antimicrobial activity against Staphylococcus aureus of hBD9 was not supressed by NaCl and MgCl2. All the antimicrobial property of the hBD9 protein against the common bacteria was verified by using conventional methods. The results improved the current knowledge on the hBD9 and its antimicrobial properties. This will drive towards the discovery of a broad spectrum, safe by effective resistant-free antibiotic in the future. The finding of this study can be used as a reference in the future investigation and it might benefits scientific community to better by others scientist or researchers to increase understanding and find new effective antibiotics.
format Thesis
author Ab Hamid, Nurul Atikah
spellingShingle Ab Hamid, Nurul Atikah
Production of novel antimicrobial peptide human beta defensin 9 and its efficacy against common eye pathogenic bacteria in different salt concentration
author_facet Ab Hamid, Nurul Atikah
author_sort Ab Hamid, Nurul Atikah
title Production of novel antimicrobial peptide human beta defensin 9 and its efficacy against common eye pathogenic bacteria in different salt concentration
title_short Production of novel antimicrobial peptide human beta defensin 9 and its efficacy against common eye pathogenic bacteria in different salt concentration
title_full Production of novel antimicrobial peptide human beta defensin 9 and its efficacy against common eye pathogenic bacteria in different salt concentration
title_fullStr Production of novel antimicrobial peptide human beta defensin 9 and its efficacy against common eye pathogenic bacteria in different salt concentration
title_full_unstemmed Production of novel antimicrobial peptide human beta defensin 9 and its efficacy against common eye pathogenic bacteria in different salt concentration
title_sort production of novel antimicrobial peptide human beta defensin 9 and its efficacy against common eye pathogenic bacteria in different salt concentration
publishDate 2018
url http://psasir.upm.edu.my/id/eprint/76290/1/FPSK%28M%29%202018%2025%20-%20IR.pdf
http://psasir.upm.edu.my/id/eprint/76290/
_version_ 1654961603226894336
score 13.211869

Similar Items