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Metagenetic analysis of gut microbial community of Malaysian mahseer Tor tambroides (Bleeker, 1854) (Cyprinidae) and its probiotics potential

Gut microbiota in vertebrate is complex and contains abundant of diverse beneficial microorganisms important for a dynamic host-microbe interaction. Some of these bacteria may have probiotics properties. In this study, the gut microbiota in wild and captive T. tambroides was identified through metag...

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Main Author: Tan, Chun Keat
Format: Thesis
Language:English
Published: 2017
Subjects:
Mahseers - Case studies - Malaysia
Freshwater fishes - Malaysia
Microbial ecology
Online Access:http://psasir.upm.edu.my/id/eprint/76118/1/FP%202018%2053%20-%20IR.pdf
http://psasir.upm.edu.my/id/eprint/76118/
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spelling my.upm.eprints.761182019-12-02T01:56:29Z http://psasir.upm.edu.my/id/eprint/76118/ Metagenetic analysis of gut microbial community of Malaysian mahseer Tor tambroides (Bleeker, 1854) (Cyprinidae) and its probiotics potential Tan, Chun Keat Gut microbiota in vertebrate is complex and contains abundant of diverse beneficial microorganisms important for a dynamic host-microbe interaction. Some of these bacteria may have probiotics properties. In this study, the gut microbiota in wild and captive T. tambroides was identified through metagenetic sequencing of 16S rDNA V3-V4 hypervariable regions using Illumina MiSeq. The sequencing data were analyzed using Quantitative Insights into Microbial Ecology (QIIME). Bacteria were isolated from gut of T. tambroides to evaluate its safety and examined for its probiotics properties. These selected potential probiotics were fed to T. tambroides followed by challenge test to evaluate its effects on growth and disease resistance against Aeromonas hydrophila. The metagenetic analyses showed that the gut microbiota in T. tambroides was dominated by Firmicutes, Proteobacteria, Fusobacteria and Bacteroidetes. Wild T. tambroides gut contained Cetobacterium sp. (24.9%), unknown genus from Peprostreptococcaceae family (11.0%), Bacteroides sp. (10.1%), PSB-M-3 from Erysipelotrichaceae family (7.89%) and Vibrio sp. (5.4%). Captive T. tambroides gut contained Cetobacterium sp. (27.9%), Citrobacter sp. (10.0%), unknown genus from Peptostreptococcaceae family (8.2%), unknown genus from Aeromonadaceae family (8.2%) and Turicibacter sp. (7.0%). The results showed that Cetobacterium sp. is the core microbiota in T. tambroides gut. Function of this bacterium in T. tambroides gut needed to be determined. Three Aeromonas sp., two Bacillus sp., two Lysinibacillus spp., and one Pseudomonas sp. were successfully isolated and identified from the wild T. tambroides gut sample. Both Bacillus sp. and Pseudomonas sp. showed quorum sensing inhibition activities while only Pseudomonas sp. showed mild antimicrobial activity against A. hydrophila. These two bacteria were selected for probiotics feeding experiment. Nevertheless, there was no significant difference in growth of T. tambroides fed with these probiotics. The T. tambroides juveniles were then challenged with A. hydrophila by intra-peritoneal injection after the probiotics feeding experiment. Both Bacillus sp. and Pseudomonas sp. appeared to be able to improve disease resistance of T. tambroides juveniles against A. hydrophila infection. Lower mortality was observed in fishes treated with Bacillus sp. and Pseudomonas sp. as compared to positive control. Lysozyme activities in T. tambroides juveniles fed with Bacillus sp. were significantly higher (P<0.05) than other treatments. In conclusion, Cetobacterium sp. is a core gut microbiota in T. tambroides. The other important gut microbiota in T. tambroides may include Bacteroides sp., Citrobacter sp., Turicibacter sp. and Bacillus sp. Bacillus sp. and Pseudomonas sp. isolated in this study showed QSI activity. Bacillus sp. could enhance the innate immune system of fishes by increase lysozyme activity in blood serum. Both Bacillus sp. and Pseudomonas sp. have the potential to be use as probiotics in T. tambroides aquaculture to improve disease resistance and immune system of fishes. 2017-12 Thesis NonPeerReviewed text en http://psasir.upm.edu.my/id/eprint/76118/1/FP%202018%2053%20-%20IR.pdf Tan, Chun Keat (2017) Metagenetic analysis of gut microbial community of Malaysian mahseer Tor tambroides (Bleeker, 1854) (Cyprinidae) and its probiotics potential. Masters thesis, Universiti Putra Malaysia. Mahseers - Case studies - Malaysia Freshwater fishes - Malaysia Microbial ecology
institution Universiti Putra Malaysia
building UPM Library
collection Institutional Repository
continent Asia
country Malaysia
content_provider Universiti Putra Malaysia
content_source UPM Institutional Repository
url_provider http://psasir.upm.edu.my/
language English
topic Mahseers - Case studies - Malaysia
Freshwater fishes - Malaysia
Microbial ecology
spellingShingle Mahseers - Case studies - Malaysia
Freshwater fishes - Malaysia
Microbial ecology
Tan, Chun Keat
Metagenetic analysis of gut microbial community of Malaysian mahseer Tor tambroides (Bleeker, 1854) (Cyprinidae) and its probiotics potential
description Gut microbiota in vertebrate is complex and contains abundant of diverse beneficial microorganisms important for a dynamic host-microbe interaction. Some of these bacteria may have probiotics properties. In this study, the gut microbiota in wild and captive T. tambroides was identified through metagenetic sequencing of 16S rDNA V3-V4 hypervariable regions using Illumina MiSeq. The sequencing data were analyzed using Quantitative Insights into Microbial Ecology (QIIME). Bacteria were isolated from gut of T. tambroides to evaluate its safety and examined for its probiotics properties. These selected potential probiotics were fed to T. tambroides followed by challenge test to evaluate its effects on growth and disease resistance against Aeromonas hydrophila. The metagenetic analyses showed that the gut microbiota in T. tambroides was dominated by Firmicutes, Proteobacteria, Fusobacteria and Bacteroidetes. Wild T. tambroides gut contained Cetobacterium sp. (24.9%), unknown genus from Peprostreptococcaceae family (11.0%), Bacteroides sp. (10.1%), PSB-M-3 from Erysipelotrichaceae family (7.89%) and Vibrio sp. (5.4%). Captive T. tambroides gut contained Cetobacterium sp. (27.9%), Citrobacter sp. (10.0%), unknown genus from Peptostreptococcaceae family (8.2%), unknown genus from Aeromonadaceae family (8.2%) and Turicibacter sp. (7.0%). The results showed that Cetobacterium sp. is the core microbiota in T. tambroides gut. Function of this bacterium in T. tambroides gut needed to be determined. Three Aeromonas sp., two Bacillus sp., two Lysinibacillus spp., and one Pseudomonas sp. were successfully isolated and identified from the wild T. tambroides gut sample. Both Bacillus sp. and Pseudomonas sp. showed quorum sensing inhibition activities while only Pseudomonas sp. showed mild antimicrobial activity against A. hydrophila. These two bacteria were selected for probiotics feeding experiment. Nevertheless, there was no significant difference in growth of T. tambroides fed with these probiotics. The T. tambroides juveniles were then challenged with A. hydrophila by intra-peritoneal injection after the probiotics feeding experiment. Both Bacillus sp. and Pseudomonas sp. appeared to be able to improve disease resistance of T. tambroides juveniles against A. hydrophila infection. Lower mortality was observed in fishes treated with Bacillus sp. and Pseudomonas sp. as compared to positive control. Lysozyme activities in T. tambroides juveniles fed with Bacillus sp. were significantly higher (P<0.05) than other treatments. In conclusion, Cetobacterium sp. is a core gut microbiota in T. tambroides. The other important gut microbiota in T. tambroides may include Bacteroides sp., Citrobacter sp., Turicibacter sp. and Bacillus sp. Bacillus sp. and Pseudomonas sp. isolated in this study showed QSI activity. Bacillus sp. could enhance the innate immune system of fishes by increase lysozyme activity in blood serum. Both Bacillus sp. and Pseudomonas sp. have the potential to be use as probiotics in T. tambroides aquaculture to improve disease resistance and immune system of fishes.
format Thesis
author Tan, Chun Keat
author_facet Tan, Chun Keat
author_sort Tan, Chun Keat
title Metagenetic analysis of gut microbial community of Malaysian mahseer Tor tambroides (Bleeker, 1854) (Cyprinidae) and its probiotics potential
title_short Metagenetic analysis of gut microbial community of Malaysian mahseer Tor tambroides (Bleeker, 1854) (Cyprinidae) and its probiotics potential
title_full Metagenetic analysis of gut microbial community of Malaysian mahseer Tor tambroides (Bleeker, 1854) (Cyprinidae) and its probiotics potential
title_fullStr Metagenetic analysis of gut microbial community of Malaysian mahseer Tor tambroides (Bleeker, 1854) (Cyprinidae) and its probiotics potential
title_full_unstemmed Metagenetic analysis of gut microbial community of Malaysian mahseer Tor tambroides (Bleeker, 1854) (Cyprinidae) and its probiotics potential
title_sort metagenetic analysis of gut microbial community of malaysian mahseer tor tambroides (bleeker, 1854) (cyprinidae) and its probiotics potential
publishDate 2017
url http://psasir.upm.edu.my/id/eprint/76118/1/FP%202018%2053%20-%20IR.pdf
http://psasir.upm.edu.my/id/eprint/76118/
_version_ 1651869276191064064
score 13.211869

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