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Isolation, Characterization and Cytotoxicity of Phytochemicals from Sekobang Kechil (Anaxagorea Javanica)

Thirty-one plant extracts were obtained from the extract bank of Natural Product Laboratory, Institute of Bioscience, Universiti Putra Malaysia. The whole plant extracts were tested for cytotoxic effect on human breast cancer (MCF-7), prostate cancer (DU-145) and lung cancer (H-460) cell lines using...

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主要作者: Zakaria, Siti Mariam
格式: Thesis
語言:English
English
出版: 2009
在線閱讀:http://psasir.upm.edu.my/id/eprint/7587/1/ABS_---_IB_2009_11.pdf
http://psasir.upm.edu.my/id/eprint/7587/
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spelling my.upm.eprints.75872013-05-27T07:35:48Z http://psasir.upm.edu.my/id/eprint/7587/ Isolation, Characterization and Cytotoxicity of Phytochemicals from Sekobang Kechil (Anaxagorea Javanica) Zakaria, Siti Mariam Thirty-one plant extracts were obtained from the extract bank of Natural Product Laboratory, Institute of Bioscience, Universiti Putra Malaysia. The whole plant extracts were tested for cytotoxic effect on human breast cancer (MCF-7), prostate cancer (DU-145) and lung cancer (H-460) cell lines using MTT assay. The results of the preliminary cytotoxicity tests showed that eight extracts exhibited very strong activity against one or more of the cell lines at 100 μg ml-1, with cell viability of 10% or less. The methanolic extract of Anaxagorea javanica leaves exhibited the strongest activity against all three cell lines with cell viability of less than 2% and further dose-response tests against the MCF-7 cell line showed that it had an IC50 value of 2.4 μg ml-1. This sample was thus selected and recollected in larger quantities for further phytochemical investigation. The dichloromethane (DCM) fraction of the first collection was subjected to chromatographic purification from which a known flavonoid, 3',4',5-trihydroxy-3,7-dimethoxyflavone (41), was obtained. From the chromatographic separation of the DCM extract of the second collection batch, a mixture of long chain alkanes, predominated by nonacosane (42) and a mixture of stigmasterol (43) and β-sitosterol (40) were isolated, in addition to three pure phytochemicals, namely an aliphatic acid, hexadecanoic acid (44), and two alkaloids, 11-methoxyeupolauridine (45) and 4,11-dimethoxyeupolauridine (46). The latter two compounds were found to be new naphthyridine alkaloids with eupolauridine nuclei and reported for the first time for this species. Compounds that had been obtained in sufficient quantities were tested for cytotoxic activity against the MCF-7 cell line. The samples assayed were 3',4',5-trihydroxy-3,7-dimethoxyflavone (41), nonacosane (42), stigmasterol (43) and β-sitosterol (40) mixture, and 11-methoxyeupolauridine (45). Only 3',4',5-trihydroxy-3,7-dimethoxyflavone (41) showed cytotoxic effect with an IC50 value of 3.4 μM, and this was its first report for this activity. Plausible biogenetic pathways of the new compounds were also discussed. 2009 Thesis NonPeerReviewed application/pdf en http://psasir.upm.edu.my/id/eprint/7587/1/ABS_---_IB_2009_11.pdf Zakaria, Siti Mariam (2009) Isolation, Characterization and Cytotoxicity of Phytochemicals from Sekobang Kechil (Anaxagorea Javanica). Masters thesis, Universiti Putra Malaysia. English
institution Universiti Putra Malaysia
building UPM Library
collection Institutional Repository
continent Asia
country Malaysia
content_provider Universiti Putra Malaysia
content_source UPM Institutional Repository
url_provider http://psasir.upm.edu.my/
language English
English
description Thirty-one plant extracts were obtained from the extract bank of Natural Product Laboratory, Institute of Bioscience, Universiti Putra Malaysia. The whole plant extracts were tested for cytotoxic effect on human breast cancer (MCF-7), prostate cancer (DU-145) and lung cancer (H-460) cell lines using MTT assay. The results of the preliminary cytotoxicity tests showed that eight extracts exhibited very strong activity against one or more of the cell lines at 100 μg ml-1, with cell viability of 10% or less. The methanolic extract of Anaxagorea javanica leaves exhibited the strongest activity against all three cell lines with cell viability of less than 2% and further dose-response tests against the MCF-7 cell line showed that it had an IC50 value of 2.4 μg ml-1. This sample was thus selected and recollected in larger quantities for further phytochemical investigation. The dichloromethane (DCM) fraction of the first collection was subjected to chromatographic purification from which a known flavonoid, 3',4',5-trihydroxy-3,7-dimethoxyflavone (41), was obtained. From the chromatographic separation of the DCM extract of the second collection batch, a mixture of long chain alkanes, predominated by nonacosane (42) and a mixture of stigmasterol (43) and β-sitosterol (40) were isolated, in addition to three pure phytochemicals, namely an aliphatic acid, hexadecanoic acid (44), and two alkaloids, 11-methoxyeupolauridine (45) and 4,11-dimethoxyeupolauridine (46). The latter two compounds were found to be new naphthyridine alkaloids with eupolauridine nuclei and reported for the first time for this species. Compounds that had been obtained in sufficient quantities were tested for cytotoxic activity against the MCF-7 cell line. The samples assayed were 3',4',5-trihydroxy-3,7-dimethoxyflavone (41), nonacosane (42), stigmasterol (43) and β-sitosterol (40) mixture, and 11-methoxyeupolauridine (45). Only 3',4',5-trihydroxy-3,7-dimethoxyflavone (41) showed cytotoxic effect with an IC50 value of 3.4 μM, and this was its first report for this activity. Plausible biogenetic pathways of the new compounds were also discussed.
format Thesis
author Zakaria, Siti Mariam
spellingShingle Zakaria, Siti Mariam
Isolation, Characterization and Cytotoxicity of Phytochemicals from Sekobang Kechil (Anaxagorea Javanica)
author_facet Zakaria, Siti Mariam
author_sort Zakaria, Siti Mariam
title Isolation, Characterization and Cytotoxicity of Phytochemicals from Sekobang Kechil (Anaxagorea Javanica)
title_short Isolation, Characterization and Cytotoxicity of Phytochemicals from Sekobang Kechil (Anaxagorea Javanica)
title_full Isolation, Characterization and Cytotoxicity of Phytochemicals from Sekobang Kechil (Anaxagorea Javanica)
title_fullStr Isolation, Characterization and Cytotoxicity of Phytochemicals from Sekobang Kechil (Anaxagorea Javanica)
title_full_unstemmed Isolation, Characterization and Cytotoxicity of Phytochemicals from Sekobang Kechil (Anaxagorea Javanica)
title_sort isolation, characterization and cytotoxicity of phytochemicals from sekobang kechil (anaxagorea javanica)
publishDate 2009
url http://psasir.upm.edu.my/id/eprint/7587/1/ABS_---_IB_2009_11.pdf
http://psasir.upm.edu.my/id/eprint/7587/
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score 13.251813

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