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Use of metabolic engineered Escherichia coli strains for enhanced biohydrogen production from palm oil mill effluent

Biological hydrogen production offers a method through which biomass can be utilized for production of premium-energy carrier as an alternative energy for replacement of non-renewable fossil fuels. The present work aims to elucidate the substrate utilization in biohydrogen production from palm oil m...

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Main Author: Taifor, Azam Fikri
Format: Thesis
Language:English
Published: 2017
Subjects:
Hydrogen - Biotechnology
Biochemical engineering
Oil palm
Online Access:http://psasir.upm.edu.my/id/eprint/75591/1/FBSB%202018%2021%20-%20IR.pdf
http://psasir.upm.edu.my/id/eprint/75591/
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spelling my.upm.eprints.755912019-11-27T01:33:17Z http://psasir.upm.edu.my/id/eprint/75591/ Use of metabolic engineered Escherichia coli strains for enhanced biohydrogen production from palm oil mill effluent Taifor, Azam Fikri Biological hydrogen production offers a method through which biomass can be utilized for production of premium-energy carrier as an alternative energy for replacement of non-renewable fossil fuels. The present work aims to elucidate the substrate utilization in biohydrogen production from palm oil mill effluent (POME) by E. coli strains. The experiments were performed in 150 mL serum bottles and the cultures were supplemented with autoclaved-pretreated POME to investigate the potential use of various carbon sources and its preference towards biohydrogen production. The cultures conditions were maintained at 37 C for 24 h with mild agitation at 120 rpm. The maximum hydrogen yield (MHY) of 0.68 mol H2 / mol total sugars with 81% substrate consumption based on total sugar and hydrogen productivity of 3552 mol / 1010 cfu were obtained from engineered E. coli BW25113 after 24 h of fermentation. The biohydrogen production by modified strain was enhanced by 3.5 fold compared to wild type strain. On the other hand, studies on the utilization of various substrates on the synthetic medium showed that the highest MHY of 0.77 mol H2 / mol total sugar was obtained from fermentation of fructose by engineered E. coli BW25113. The preference of the substrates based on the synthetic medium for biohydrogen production was in the following order; fructose > glucose > formic acid. These findings indicated that the use of modified strain E. coli BW25113 has enhanced biohydrogen production from POME. 2017-11 Thesis NonPeerReviewed text en http://psasir.upm.edu.my/id/eprint/75591/1/FBSB%202018%2021%20-%20IR.pdf Taifor, Azam Fikri (2017) Use of metabolic engineered Escherichia coli strains for enhanced biohydrogen production from palm oil mill effluent. Masters thesis, Universiti Putra Malaysia. Hydrogen - Biotechnology Biochemical engineering Oil palm
institution Universiti Putra Malaysia
building UPM Library
collection Institutional Repository
continent Asia
country Malaysia
content_provider Universiti Putra Malaysia
content_source UPM Institutional Repository
url_provider http://psasir.upm.edu.my/
language English
topic Hydrogen - Biotechnology
Biochemical engineering
Oil palm
spellingShingle Hydrogen - Biotechnology
Biochemical engineering
Oil palm
Taifor, Azam Fikri
Use of metabolic engineered Escherichia coli strains for enhanced biohydrogen production from palm oil mill effluent
description Biological hydrogen production offers a method through which biomass can be utilized for production of premium-energy carrier as an alternative energy for replacement of non-renewable fossil fuels. The present work aims to elucidate the substrate utilization in biohydrogen production from palm oil mill effluent (POME) by E. coli strains. The experiments were performed in 150 mL serum bottles and the cultures were supplemented with autoclaved-pretreated POME to investigate the potential use of various carbon sources and its preference towards biohydrogen production. The cultures conditions were maintained at 37 C for 24 h with mild agitation at 120 rpm. The maximum hydrogen yield (MHY) of 0.68 mol H2 / mol total sugars with 81% substrate consumption based on total sugar and hydrogen productivity of 3552 mol / 1010 cfu were obtained from engineered E. coli BW25113 after 24 h of fermentation. The biohydrogen production by modified strain was enhanced by 3.5 fold compared to wild type strain. On the other hand, studies on the utilization of various substrates on the synthetic medium showed that the highest MHY of 0.77 mol H2 / mol total sugar was obtained from fermentation of fructose by engineered E. coli BW25113. The preference of the substrates based on the synthetic medium for biohydrogen production was in the following order; fructose > glucose > formic acid. These findings indicated that the use of modified strain E. coli BW25113 has enhanced biohydrogen production from POME.
format Thesis
author Taifor, Azam Fikri
author_facet Taifor, Azam Fikri
author_sort Taifor, Azam Fikri
title Use of metabolic engineered Escherichia coli strains for enhanced biohydrogen production from palm oil mill effluent
title_short Use of metabolic engineered Escherichia coli strains for enhanced biohydrogen production from palm oil mill effluent
title_full Use of metabolic engineered Escherichia coli strains for enhanced biohydrogen production from palm oil mill effluent
title_fullStr Use of metabolic engineered Escherichia coli strains for enhanced biohydrogen production from palm oil mill effluent
title_full_unstemmed Use of metabolic engineered Escherichia coli strains for enhanced biohydrogen production from palm oil mill effluent
title_sort use of metabolic engineered escherichia coli strains for enhanced biohydrogen production from palm oil mill effluent
publishDate 2017
url http://psasir.upm.edu.my/id/eprint/75591/1/FBSB%202018%2021%20-%20IR.pdf
http://psasir.upm.edu.my/id/eprint/75591/
_version_ 1651869198962393088
score 13.211869

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