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Anti-nociceptive and anti-inflammatory properties of Melicope ptelefolia Champ ex Benth ethanolic extract

Melicope ptelefolia, locally known as ‘tenggek burung’, is consumed for various purposes in folk medicine such as treating fever, pain, wounds and itches. In present study, investigation of anti-nociceptive and anti-inflammatory properties of MPEE was conducted in nociception and inflammation-induce...

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Main Author: Mohd Padzil, Azyyati
Format: Thesis
Language:English
Published: 2013
Subjects:
Biological Products - adverse effects
Plants, Medicinal - toxicity
Biological Products - chemical synthesis
Online Access:http://psasir.upm.edu.my/id/eprint/75331/1/FPSK%28M%29%202013%2056%20IR.pdf
http://psasir.upm.edu.my/id/eprint/75331/
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spelling my.upm.eprints.753312019-11-21T08:11:26Z http://psasir.upm.edu.my/id/eprint/75331/ Anti-nociceptive and anti-inflammatory properties of Melicope ptelefolia Champ ex Benth ethanolic extract Mohd Padzil, Azyyati Melicope ptelefolia, locally known as ‘tenggek burung’, is consumed for various purposes in folk medicine such as treating fever, pain, wounds and itches. In present study, investigation of anti-nociceptive and anti-inflammatory properties of MPEE was conducted in nociception and inflammation-induced murine models, respectively. The anti-nociceptive activity of the extract was assessed using acetic acid-induced abdominal writhing, hot plate and formalin-induced paw licking tests. Meanwhile, studies on inhibition of acute inflammation and underlying mechanisms were conducted via paw edema model, using 1% carrageenan and different inflammatory mediators as edemogen. Investigation on the potent anti-inflammatory effects was also conducted on cotton pellet induced granuloma test models. Toxicity analysis of MPEE was also conducted. Oral administration of MPEE produced significant dose-dependent anti-nociceptive effects when tested in mice and rats using acetic acid-induced abdominal constrictiontest and on the second phase of the formalin induced paw licking test, respectively. It was also demonstrated that MPEE had no effect on the response latency time to the heat stimulus in the thermal model of the hot-plate test. Additionally, MPEE anti-nociception was not reversed by pre-administration of naloxone. MPEE anti-nociception mechanism of action was demonstrated on the inhibition of glutamate induced paw licking test but not capsaicin. For anti-inflammatory activity, ethanol extract of Melicope ptelefolia significantly inhibited formation of edema throughout 390 minutes of edema formation. For inflammatory mediators - induced paw edema test, MPEE showed significant reduction in histamine (66.67%), serotonin (54.9%), arachidonic acid (65.63%), and prostaglandin (40%) - induced paw edema test. For chronic inflammatory test model, highest concentration of MPEE (300 mg/kg, p.o) inhibited both wet and dry weight of cotton pellet while not significantly elevating the value of three biochemical markers (ALT, ALP and total protein). Furthermore, oral administration of MPEE did not produce any significant effect on balance and motor coordination in rotarod test. In acute toxicity study, sign of toxicity was detected in liver tissue for dose of 1000 mg/kg MPEE. These results indicated that MPEE at all investigated doses exerted pronounced anti-nociceptive activity that acts peripherally in experimental animals and did not produce any effects on motor corrdination and balance. MPEE also is considered safe until the concentration of 300 mg/kg. 2013-10 Thesis NonPeerReviewed text en http://psasir.upm.edu.my/id/eprint/75331/1/FPSK%28M%29%202013%2056%20IR.pdf Mohd Padzil, Azyyati (2013) Anti-nociceptive and anti-inflammatory properties of Melicope ptelefolia Champ ex Benth ethanolic extract. Masters thesis, Universiti Putra Malaysia. Biological Products - adverse effects Plants, Medicinal - toxicity Biological Products - chemical synthesis
institution Universiti Putra Malaysia
building UPM Library
collection Institutional Repository
continent Asia
country Malaysia
content_provider Universiti Putra Malaysia
content_source UPM Institutional Repository
url_provider http://psasir.upm.edu.my/
language English
topic Biological Products - adverse effects
Plants, Medicinal - toxicity
Biological Products - chemical synthesis
spellingShingle Biological Products - adverse effects
Plants, Medicinal - toxicity
Biological Products - chemical synthesis
Mohd Padzil, Azyyati
Anti-nociceptive and anti-inflammatory properties of Melicope ptelefolia Champ ex Benth ethanolic extract
description Melicope ptelefolia, locally known as ‘tenggek burung’, is consumed for various purposes in folk medicine such as treating fever, pain, wounds and itches. In present study, investigation of anti-nociceptive and anti-inflammatory properties of MPEE was conducted in nociception and inflammation-induced murine models, respectively. The anti-nociceptive activity of the extract was assessed using acetic acid-induced abdominal writhing, hot plate and formalin-induced paw licking tests. Meanwhile, studies on inhibition of acute inflammation and underlying mechanisms were conducted via paw edema model, using 1% carrageenan and different inflammatory mediators as edemogen. Investigation on the potent anti-inflammatory effects was also conducted on cotton pellet induced granuloma test models. Toxicity analysis of MPEE was also conducted. Oral administration of MPEE produced significant dose-dependent anti-nociceptive effects when tested in mice and rats using acetic acid-induced abdominal constrictiontest and on the second phase of the formalin induced paw licking test, respectively. It was also demonstrated that MPEE had no effect on the response latency time to the heat stimulus in the thermal model of the hot-plate test. Additionally, MPEE anti-nociception was not reversed by pre-administration of naloxone. MPEE anti-nociception mechanism of action was demonstrated on the inhibition of glutamate induced paw licking test but not capsaicin. For anti-inflammatory activity, ethanol extract of Melicope ptelefolia significantly inhibited formation of edema throughout 390 minutes of edema formation. For inflammatory mediators - induced paw edema test, MPEE showed significant reduction in histamine (66.67%), serotonin (54.9%), arachidonic acid (65.63%), and prostaglandin (40%) - induced paw edema test. For chronic inflammatory test model, highest concentration of MPEE (300 mg/kg, p.o) inhibited both wet and dry weight of cotton pellet while not significantly elevating the value of three biochemical markers (ALT, ALP and total protein). Furthermore, oral administration of MPEE did not produce any significant effect on balance and motor coordination in rotarod test. In acute toxicity study, sign of toxicity was detected in liver tissue for dose of 1000 mg/kg MPEE. These results indicated that MPEE at all investigated doses exerted pronounced anti-nociceptive activity that acts peripherally in experimental animals and did not produce any effects on motor corrdination and balance. MPEE also is considered safe until the concentration of 300 mg/kg.
format Thesis
author Mohd Padzil, Azyyati
author_facet Mohd Padzil, Azyyati
author_sort Mohd Padzil, Azyyati
title Anti-nociceptive and anti-inflammatory properties of Melicope ptelefolia Champ ex Benth ethanolic extract
title_short Anti-nociceptive and anti-inflammatory properties of Melicope ptelefolia Champ ex Benth ethanolic extract
title_full Anti-nociceptive and anti-inflammatory properties of Melicope ptelefolia Champ ex Benth ethanolic extract
title_fullStr Anti-nociceptive and anti-inflammatory properties of Melicope ptelefolia Champ ex Benth ethanolic extract
title_full_unstemmed Anti-nociceptive and anti-inflammatory properties of Melicope ptelefolia Champ ex Benth ethanolic extract
title_sort anti-nociceptive and anti-inflammatory properties of melicope ptelefolia champ ex benth ethanolic extract
publishDate 2013
url http://psasir.upm.edu.my/id/eprint/75331/1/FPSK%28M%29%202013%2056%20IR.pdf
http://psasir.upm.edu.my/id/eprint/75331/
_version_ 1651869182259625984
score 13.222552

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