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Utilization of the internal transcribed spacer (ITS) DNA sequence to trace the geographical sources of Aquilaria malaccensis Lam. populations

Overexploitation in search of its valuable non-wood fragrance product has put pressure on the survival of the endangered Aquilaria trees in the wild. In this study, the pattern of genetic variation among wild populations was measured to aid in designing useful strategies for in situ conservation. We...

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Main Authors: Lee, Shiou Yih, Mohamed, Rozi, Ibrahim, Faridah Hanum, Lamasudin, Dhilia Udie
Format: Article
Language:English
Published: National Institute of Agricultural Botany (NIAB) 2018
Online Access:http://psasir.upm.edu.my/id/eprint/74389/1/Utilization%20of%20the%20internal%20transcribed%20spacer%20%28ITS%29%20DNA%20sequence%20to%20trace%20the%20geographical%20sources%20of%20Aquilaria%20malaccensis%20Lam.%20populations.pdf
http://psasir.upm.edu.my/id/eprint/74389/
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spelling my.upm.eprints.743892020-03-26T07:59:05Z http://psasir.upm.edu.my/id/eprint/74389/ Utilization of the internal transcribed spacer (ITS) DNA sequence to trace the geographical sources of Aquilaria malaccensis Lam. populations Lee, Shiou Yih Mohamed, Rozi Ibrahim, Faridah Hanum Lamasudin, Dhilia Udie Overexploitation in search of its valuable non-wood fragrance product has put pressure on the survival of the endangered Aquilaria trees in the wild. In this study, the pattern of genetic variation among wild populations was measured to aid in designing useful strategies for in situ conservation. We sequenced the internal transcribed spacer (ITS) of the nuclear region of 19 wild Aquilaria malaccensis populations from different states in Peninsular Malaysia, and compared the sequence with the same species residing outside of Malaysia, mainly from the Assam region in India and Sumatra in Indonesia. This widely distributed species is found in the Indomalesian region and is a major source of agarwood. In addition, we included five wild Aquilaria hirta populations for semblance purposes. Intraspecific variations were not found within A. malaccensis and A. hirta populations in Peninsular Malaysia. Interestingly, Single Nucleotide polymorphisms (SNPs) were identified when comparing A. malaccensis from three geographical regions, with a total of 25 SNPs detected. We imply that geographical segregation is a contributing factor toward genetic variation in A. malaccensis. This is the first report on utilizing the ITS region for analysing genetic variation in A. malaccensis of various geographical regions. The molecular information obtained in this study will serve as a useful reference in designing in situ programmes for this endangered species. National Institute of Agricultural Botany (NIAB) 2018 Article PeerReviewed text en http://psasir.upm.edu.my/id/eprint/74389/1/Utilization%20of%20the%20internal%20transcribed%20spacer%20%28ITS%29%20DNA%20sequence%20to%20trace%20the%20geographical%20sources%20of%20Aquilaria%20malaccensis%20Lam.%20populations.pdf Lee, Shiou Yih and Mohamed, Rozi and Ibrahim, Faridah Hanum and Lamasudin, Dhilia Udie (2018) Utilization of the internal transcribed spacer (ITS) DNA sequence to trace the geographical sources of Aquilaria malaccensis Lam. populations. Plant Genetic Resources, 16 (2). 103 - 111. ISSN 1479-2621; EISSN: 1479-263X 10.1017/S1479262117000016
institution Universiti Putra Malaysia
building UPM Library
collection Institutional Repository
continent Asia
country Malaysia
content_provider Universiti Putra Malaysia
content_source UPM Institutional Repository
url_provider http://psasir.upm.edu.my/
language English
description Overexploitation in search of its valuable non-wood fragrance product has put pressure on the survival of the endangered Aquilaria trees in the wild. In this study, the pattern of genetic variation among wild populations was measured to aid in designing useful strategies for in situ conservation. We sequenced the internal transcribed spacer (ITS) of the nuclear region of 19 wild Aquilaria malaccensis populations from different states in Peninsular Malaysia, and compared the sequence with the same species residing outside of Malaysia, mainly from the Assam region in India and Sumatra in Indonesia. This widely distributed species is found in the Indomalesian region and is a major source of agarwood. In addition, we included five wild Aquilaria hirta populations for semblance purposes. Intraspecific variations were not found within A. malaccensis and A. hirta populations in Peninsular Malaysia. Interestingly, Single Nucleotide polymorphisms (SNPs) were identified when comparing A. malaccensis from three geographical regions, with a total of 25 SNPs detected. We imply that geographical segregation is a contributing factor toward genetic variation in A. malaccensis. This is the first report on utilizing the ITS region for analysing genetic variation in A. malaccensis of various geographical regions. The molecular information obtained in this study will serve as a useful reference in designing in situ programmes for this endangered species.
format Article
author Lee, Shiou Yih
Mohamed, Rozi
Ibrahim, Faridah Hanum
Lamasudin, Dhilia Udie
spellingShingle Lee, Shiou Yih
Mohamed, Rozi
Ibrahim, Faridah Hanum
Lamasudin, Dhilia Udie
Utilization of the internal transcribed spacer (ITS) DNA sequence to trace the geographical sources of Aquilaria malaccensis Lam. populations
author_facet Lee, Shiou Yih
Mohamed, Rozi
Ibrahim, Faridah Hanum
Lamasudin, Dhilia Udie
author_sort Lee, Shiou Yih
title Utilization of the internal transcribed spacer (ITS) DNA sequence to trace the geographical sources of Aquilaria malaccensis Lam. populations
title_short Utilization of the internal transcribed spacer (ITS) DNA sequence to trace the geographical sources of Aquilaria malaccensis Lam. populations
title_full Utilization of the internal transcribed spacer (ITS) DNA sequence to trace the geographical sources of Aquilaria malaccensis Lam. populations
title_fullStr Utilization of the internal transcribed spacer (ITS) DNA sequence to trace the geographical sources of Aquilaria malaccensis Lam. populations
title_full_unstemmed Utilization of the internal transcribed spacer (ITS) DNA sequence to trace the geographical sources of Aquilaria malaccensis Lam. populations
title_sort utilization of the internal transcribed spacer (its) dna sequence to trace the geographical sources of aquilaria malaccensis lam. populations
publisher National Institute of Agricultural Botany (NIAB)
publishDate 2018
url http://psasir.upm.edu.my/id/eprint/74389/1/Utilization%20of%20the%20internal%20transcribed%20spacer%20%28ITS%29%20DNA%20sequence%20to%20trace%20the%20geographical%20sources%20of%20Aquilaria%20malaccensis%20Lam.%20populations.pdf
http://psasir.upm.edu.my/id/eprint/74389/
_version_ 1665896019819233280
score 13.211869

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