Staff View: The effect of DMEM and DMEM:F12 culture media on the growth of SH-SY5Y cells

The effect of DMEM and DMEM:F12 culture media on the growth of SH-SY5Y cells

The human neuroblastoma cell line, SH-SY5Y cells, is commonly used as an in vitro model for neuroscience and neurobiology research. SH-SY5Y cells were established during the 1970’s from the parental metastatic bone tumor, SK-N-SH cell line. The utilization of SH-SY5Y cells in neurobiology research i...

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Main Authors:	Mohammed Izham, Aishah, Chia, Jasmine Siew Min, Vidyadaran, Sharmili, Sulaiman, Mohd Roslan, Bharatham, Hemabarathy, Perimal, Enoch Kumar
Format:	Article
Language:	English
Published:	Biome Scientia 2018
Online Access:	http://psasir.upm.edu.my/id/eprint/74142/1/The%20effect%20of%20DMEM%20and%20DMEM.pdf http://psasir.upm.edu.my/id/eprint/74142/ https://biomescientia.com/index.php/lsmb/article/view/23 https://doi.org/10.28916/lsmb.2.3.2018.23
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spelling	my.upm.eprints.741422021-09-22T21:30:21Z http://psasir.upm.edu.my/id/eprint/74142/ The effect of DMEM and DMEM:F12 culture media on the growth of SH-SY5Y cells Mohammed Izham, Aishah Chia, Jasmine Siew Min Vidyadaran, Sharmili Sulaiman, Mohd Roslan Bharatham, Hemabarathy Perimal, Enoch Kumar The human neuroblastoma cell line, SH-SY5Y cells, is commonly used as an in vitro model for neuroscience and neurobiology research. SH-SY5Y cells were established during the 1970’s from the parental metastatic bone tumor, SK-N-SH cell line. The utilization of SH-SY5Y cells in neurobiology research is due to their resemblance to the human primary neuronal cells with several limitations, considering the cells are derived from malignant tumor cells. Since SH-SY5Y cells are widely cultured for research, several different culture media have been used to optimize the growth of the cells, including Eagle's Minimum Essential Medium (EMEM), Dulbecco’s modified Eagle’s medium (DMEM) and other recently developed culture media. The ability of SH-SY5Y cells to reach confluency in culture flasks ranges from 5 days to 15 days, depending on the culture media. Hence, the optimization of the culture media is crucial to achieve the fastest growth rate for the cells. The objective of the study is to evaluate the culture media for the proliferation of SH-SY5Y cells. We compared the growth rate of SH-SY5Y cells cultured in Dulbecco’s modified Eagle’s medium (DMEM) supplemented with 15% heat-inactivated fetal bovine serum (hiFBS), Dulbecco’s modified Eagle’s medium: Nutrientmixture F-12 (DMEM:F12) + supplemented with 15% hiFBS and DMEM:F12 supplemented with 10% hiFBS. In DMEM:F12 supplemented with 15% hiFBS, cells grew up to 6.67E+05 cells. In DMEM:F12 supplemented with 10%hiFBS, cells grew up to 5.28E+05 cells. In DMEM supplemented with 15% hiFBS, the cells grew up to 4.76E+05 cells. There was a significant difference between culture media DMEM:F12 supplemented with 15% hiFBS as compared to DMEM:F12 supplemented with 10%hiFBS and DMEM supplemented with 15% hiFBS (p0.05). Biome Scientia 2018-12 Article PeerReviewed text en http://psasir.upm.edu.my/id/eprint/74142/1/The%20effect%20of%20DMEM%20and%20DMEM.pdf Mohammed Izham, Aishah and Chia, Jasmine Siew Min and Vidyadaran, Sharmili and Sulaiman, Mohd Roslan and Bharatham, Hemabarathy and Perimal, Enoch Kumar (2018) The effect of DMEM and DMEM:F12 culture media on the growth of SH-SY5Y cells. Life Sciences, Medicine and Biomedicine, 2 (3). art. no. 23. pp. 1-4. ISSN 2600-7207 https://biomescientia.com/index.php/lsmb/article/view/23 https://doi.org/10.28916/lsmb.2.3.2018.23
institution	Universiti Putra Malaysia
building	UPM Library
collection	Institutional Repository
continent	Asia
country	Malaysia
content_provider	Universiti Putra Malaysia
content_source	UPM Institutional Repository
url_provider	http://psasir.upm.edu.my/
language	English
description	The human neuroblastoma cell line, SH-SY5Y cells, is commonly used as an in vitro model for neuroscience and neurobiology research. SH-SY5Y cells were established during the 1970’s from the parental metastatic bone tumor, SK-N-SH cell line. The utilization of SH-SY5Y cells in neurobiology research is due to their resemblance to the human primary neuronal cells with several limitations, considering the cells are derived from malignant tumor cells. Since SH-SY5Y cells are widely cultured for research, several different culture media have been used to optimize the growth of the cells, including Eagle's Minimum Essential Medium (EMEM), Dulbecco’s modified Eagle’s medium (DMEM) and other recently developed culture media. The ability of SH-SY5Y cells to reach confluency in culture flasks ranges from 5 days to 15 days, depending on the culture media. Hence, the optimization of the culture media is crucial to achieve the fastest growth rate for the cells. The objective of the study is to evaluate the culture media for the proliferation of SH-SY5Y cells. We compared the growth rate of SH-SY5Y cells cultured in Dulbecco’s modified Eagle’s medium (DMEM) supplemented with 15% heat-inactivated fetal bovine serum (hiFBS), Dulbecco’s modified Eagle’s medium: Nutrientmixture F-12 (DMEM:F12) + supplemented with 15% hiFBS and DMEM:F12 supplemented with 10% hiFBS. In DMEM:F12 supplemented with 15% hiFBS, cells grew up to 6.67E+05 cells. In DMEM:F12 supplemented with 10%hiFBS, cells grew up to 5.28E+05 cells. In DMEM supplemented with 15% hiFBS, the cells grew up to 4.76E+05 cells. There was a significant difference between culture media DMEM:F12 supplemented with 15% hiFBS as compared to DMEM:F12 supplemented with 10%hiFBS and DMEM supplemented with 15% hiFBS (p0.05).
format	Article
author	Mohammed Izham, Aishah Chia, Jasmine Siew Min Vidyadaran, Sharmili Sulaiman, Mohd Roslan Bharatham, Hemabarathy Perimal, Enoch Kumar
spellingShingle	Mohammed Izham, Aishah Chia, Jasmine Siew Min Vidyadaran, Sharmili Sulaiman, Mohd Roslan Bharatham, Hemabarathy Perimal, Enoch Kumar The effect of DMEM and DMEM:F12 culture media on the growth of SH-SY5Y cells
author_facet	Mohammed Izham, Aishah Chia, Jasmine Siew Min Vidyadaran, Sharmili Sulaiman, Mohd Roslan Bharatham, Hemabarathy Perimal, Enoch Kumar
author_sort	Mohammed Izham, Aishah
title	The effect of DMEM and DMEM:F12 culture media on the growth of SH-SY5Y cells
title_short	The effect of DMEM and DMEM:F12 culture media on the growth of SH-SY5Y cells
title_full	The effect of DMEM and DMEM:F12 culture media on the growth of SH-SY5Y cells
title_fullStr	The effect of DMEM and DMEM:F12 culture media on the growth of SH-SY5Y cells
title_full_unstemmed	The effect of DMEM and DMEM:F12 culture media on the growth of SH-SY5Y cells
title_sort	effect of dmem and dmem:f12 culture media on the growth of sh-sy5y cells
publisher	Biome Scientia
publishDate	2018
url	http://psasir.upm.edu.my/id/eprint/74142/1/The%20effect%20of%20DMEM%20and%20DMEM.pdf http://psasir.upm.edu.my/id/eprint/74142/ https://biomescientia.com/index.php/lsmb/article/view/23 https://doi.org/10.28916/lsmb.2.3.2018.23
_version_	1712286726552027136
score	13.211869

The effect of DMEM and DMEM:F12 culture media on the growth of SH-SY5Y cells

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