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Responses of haptoglobin and serum amyloid A in goats inoculated intradermally with C. pseudotuberculosis and mycolic acid extract immunogen

Haptoglobin (Hp) and Serum Amyloid A (SAA) are a group of blood proteins whose concentrations in animals can be influenced by infection, inflammation, surgical trauma or stress. Corynebacterium pseudotuberculosis is the causative agent of caseous lymphadenitis (CLA), and Mycolic acid is a virulent f...

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Main Authors: Ahmed Odhah, Mohammed Naji, Abdullah, Faiz Firdaus Jesse, Adamu, Lawan, U. H., Idris, Marza, Ali Dhiaa, Mahmood, Zaid Khudhur, A., Yusuf, Maqbool, Arsalan, Haron, Abd Wahid, Mohd Lila, Mohd Azmi, Saad, Mohammad Zamri
Format: Article
Language:English
Published: Elsevier 2018
Online Access:http://psasir.upm.edu.my/id/eprint/73779/1/Responses%20of%20haptoglobin%20and%20serum%20amyloid%20A%20in%20goats%20inoculated%20intradermally%20with%20C.%20pseudotuberculosis%20and%20mycolic%20acid%20extract%20immunogen.pdf
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spelling my.upm.eprints.737792020-05-08T16:27:32Z http://psasir.upm.edu.my/id/eprint/73779/ Responses of haptoglobin and serum amyloid A in goats inoculated intradermally with C. pseudotuberculosis and mycolic acid extract immunogen Ahmed Odhah, Mohammed Naji Abdullah, Faiz Firdaus Jesse Adamu, Lawan U. H., Idris Marza, Ali Dhiaa Mahmood, Zaid Khudhur A., Yusuf Maqbool, Arsalan Haron, Abd Wahid Mohd Lila, Mohd Azmi Saad, Mohammad Zamri Haptoglobin (Hp) and Serum Amyloid A (SAA) are a group of blood proteins whose concentrations in animals can be influenced by infection, inflammation, surgical trauma or stress. Corynebacterium pseudotuberculosis is the causative agent of caseous lymphadenitis (CLA), and Mycolic acid is a virulent factor extracted from C. pseudotuberculosis. There is a dearth of sufficient evidence on the clinical implication of MAs on the responses of Hp and SAA in goats. Therefore, this study was conducted to evaluate the potential effects of Mycolic acid (MAs) and C. pseudotuberculosis on the responses of Hp and SAA in female goats. A total of 12 healthy female goats was divided into three groups; A, B and C each comprising of 4 goats and managed for a period of three months. Group (A) was inoculated with 2 mL of sterile phosphate buffered saline (as a negative control group) intradermally, while group (B) and (C) were inoculated intradermally with 2 ml each of mycolic acid and 1‏ × 109 cfu of active C. pseudotuberculosis respectively. The result of the study showed that the Hp concentration in goats inoculated with C. pseudotuberculosis was significantly increased up to 7-fold (1.17 ± 0.17 ng/L) while MAs showed a 3-fold increased (0.83 ± 0.01 ng/L) compared with the control. Whereas SAA concentration in C. pseudotuberculosis and MAs groups showed a significant 3-fold (17.85 ± 0.91 pg/mL) and 2-fold (10.97 ± 0.71 pg/mL) increased compared with the control. This study concludes that inoculation of C. pseudotuberculosis and MAs have significant effects on Hp and SAA levels, which indicates that MAs could have a role in the pathogenesis of caseous lymphadenitis. Elsevier 2018 Article PeerReviewed text en http://psasir.upm.edu.my/id/eprint/73779/1/Responses%20of%20haptoglobin%20and%20serum%20amyloid%20A%20in%20goats%20inoculated%20intradermally%20with%20C.%20pseudotuberculosis%20and%20mycolic%20acid%20extract%20immunogen.pdf Ahmed Odhah, Mohammed Naji and Abdullah, Faiz Firdaus Jesse and Adamu, Lawan and U. H., Idris and Marza, Ali Dhiaa and Mahmood, Zaid Khudhur and A., Yusuf and Maqbool, Arsalan and Haron, Abd Wahid and Mohd Lila, Mohd Azmi and Saad, Mohammad Zamri (2018) Responses of haptoglobin and serum amyloid A in goats inoculated intradermally with C. pseudotuberculosis and mycolic acid extract immunogen. Microbial Pathogenesis, 117. 243 - 246. ISSN 0882-4010; ESSN: 1096-1208 10.1016/j.micpath.2018.02.038
institution Universiti Putra Malaysia
building UPM Library
collection Institutional Repository
continent Asia
country Malaysia
content_provider Universiti Putra Malaysia
content_source UPM Institutional Repository
url_provider http://psasir.upm.edu.my/
language English
description Haptoglobin (Hp) and Serum Amyloid A (SAA) are a group of blood proteins whose concentrations in animals can be influenced by infection, inflammation, surgical trauma or stress. Corynebacterium pseudotuberculosis is the causative agent of caseous lymphadenitis (CLA), and Mycolic acid is a virulent factor extracted from C. pseudotuberculosis. There is a dearth of sufficient evidence on the clinical implication of MAs on the responses of Hp and SAA in goats. Therefore, this study was conducted to evaluate the potential effects of Mycolic acid (MAs) and C. pseudotuberculosis on the responses of Hp and SAA in female goats. A total of 12 healthy female goats was divided into three groups; A, B and C each comprising of 4 goats and managed for a period of three months. Group (A) was inoculated with 2 mL of sterile phosphate buffered saline (as a negative control group) intradermally, while group (B) and (C) were inoculated intradermally with 2 ml each of mycolic acid and 1‏ × 109 cfu of active C. pseudotuberculosis respectively. The result of the study showed that the Hp concentration in goats inoculated with C. pseudotuberculosis was significantly increased up to 7-fold (1.17 ± 0.17 ng/L) while MAs showed a 3-fold increased (0.83 ± 0.01 ng/L) compared with the control. Whereas SAA concentration in C. pseudotuberculosis and MAs groups showed a significant 3-fold (17.85 ± 0.91 pg/mL) and 2-fold (10.97 ± 0.71 pg/mL) increased compared with the control. This study concludes that inoculation of C. pseudotuberculosis and MAs have significant effects on Hp and SAA levels, which indicates that MAs could have a role in the pathogenesis of caseous lymphadenitis.
format Article
author Ahmed Odhah, Mohammed Naji
Abdullah, Faiz Firdaus Jesse
Adamu, Lawan
U. H., Idris
Marza, Ali Dhiaa
Mahmood, Zaid Khudhur
A., Yusuf
Maqbool, Arsalan
Haron, Abd Wahid
Mohd Lila, Mohd Azmi
Saad, Mohammad Zamri
spellingShingle Ahmed Odhah, Mohammed Naji
Abdullah, Faiz Firdaus Jesse
Adamu, Lawan
U. H., Idris
Marza, Ali Dhiaa
Mahmood, Zaid Khudhur
A., Yusuf
Maqbool, Arsalan
Haron, Abd Wahid
Mohd Lila, Mohd Azmi
Saad, Mohammad Zamri
Responses of haptoglobin and serum amyloid A in goats inoculated intradermally with C. pseudotuberculosis and mycolic acid extract immunogen
author_facet Ahmed Odhah, Mohammed Naji
Abdullah, Faiz Firdaus Jesse
Adamu, Lawan
U. H., Idris
Marza, Ali Dhiaa
Mahmood, Zaid Khudhur
A., Yusuf
Maqbool, Arsalan
Haron, Abd Wahid
Mohd Lila, Mohd Azmi
Saad, Mohammad Zamri
author_sort Ahmed Odhah, Mohammed Naji
title Responses of haptoglobin and serum amyloid A in goats inoculated intradermally with C. pseudotuberculosis and mycolic acid extract immunogen
title_short Responses of haptoglobin and serum amyloid A in goats inoculated intradermally with C. pseudotuberculosis and mycolic acid extract immunogen
title_full Responses of haptoglobin and serum amyloid A in goats inoculated intradermally with C. pseudotuberculosis and mycolic acid extract immunogen
title_fullStr Responses of haptoglobin and serum amyloid A in goats inoculated intradermally with C. pseudotuberculosis and mycolic acid extract immunogen
title_full_unstemmed Responses of haptoglobin and serum amyloid A in goats inoculated intradermally with C. pseudotuberculosis and mycolic acid extract immunogen
title_sort responses of haptoglobin and serum amyloid a in goats inoculated intradermally with c. pseudotuberculosis and mycolic acid extract immunogen
publisher Elsevier
publishDate 2018
url http://psasir.upm.edu.my/id/eprint/73779/1/Responses%20of%20haptoglobin%20and%20serum%20amyloid%20A%20in%20goats%20inoculated%20intradermally%20with%20C.%20pseudotuberculosis%20and%20mycolic%20acid%20extract%20immunogen.pdf
http://psasir.upm.edu.my/id/eprint/73779/
_version_ 1669008831590432768
score 13.211869

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