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Regulation of thiamine biosynthesis upon exogenous application of the vitamin in oil palm (Elaies guineensis)

Thiamine (vitamin B1) is an essential microelement that is synthesised de novo by plants and microorganisms. The active form of thiamine is thiamine pyrophosphate (TPP), which plays a prominent role in plant's metabolic activity particularly as an enzymatic cofactor. In vivo analysis of thiamin...

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Main Authors: Subki, Atiqah, Khairi Thaw, Siti Fariedda, Balia Yusof, Zetty Norhana
Format: Article
Language:English
Published: Malaysian Palm Oil Board 2018
Online Access:http://psasir.upm.edu.my/id/eprint/73736/1/Regulation%20of%20thiamine%20biosynthesis%20upon%20exogenous%20application%20of%20the%20vitamin%20in%20oil%20palm.pdf
http://psasir.upm.edu.my/id/eprint/73736/
http://jopr.mpob.gov.my/regulation-of-thiamine-biosynthesis-upon-exogenous-application-of-the-vitamin-in-oil-palm-elaeis-guineensis/
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spelling my.upm.eprints.737362021-07-15T23:58:24Z http://psasir.upm.edu.my/id/eprint/73736/ Regulation of thiamine biosynthesis upon exogenous application of the vitamin in oil palm (Elaies guineensis) Subki, Atiqah Khairi Thaw, Siti Fariedda Balia Yusof, Zetty Norhana Thiamine (vitamin B1) is an essential microelement that is synthesised de novo by plants and microorganisms. The active form of thiamine is thiamine pyrophosphate (TPP), which plays a prominent role in plant's metabolic activity particularly as an enzymatic cofactor. In vivo analysis of thiamine in oil palm was performed where four months old oil palm seedlings were treated with 125 ml of 50 mM thiamine hydrochloride and tissue samples were collected at Day 0, 1, 2 and 3. The expression of ThiC gene fragment was analysed via quantitative real time polymerase chain reaction (qPCR) which showed an approximately five-fold decrease post-thiamine treatment. Analysis of thiamine and its derivatives via high performance liquid chromatography (HPLC) showed that the concentration of thiamine decreased, while TPP increased post-thiamine treatment. It is suggested that thiamine biosynthesis in oil palm can be effectively regulated at the physiological concentrations of the vitamin. Malaysian Palm Oil Board 2018-06 Article PeerReviewed text en http://psasir.upm.edu.my/id/eprint/73736/1/Regulation%20of%20thiamine%20biosynthesis%20upon%20exogenous%20application%20of%20the%20vitamin%20in%20oil%20palm.pdf Subki, Atiqah and Khairi Thaw, Siti Fariedda and Balia Yusof, Zetty Norhana (2018) Regulation of thiamine biosynthesis upon exogenous application of the vitamin in oil palm (Elaies guineensis). Journal of Oil Palm Research, 30 (2). 236 - 241. ISSN 1511-2780 http://jopr.mpob.gov.my/regulation-of-thiamine-biosynthesis-upon-exogenous-application-of-the-vitamin-in-oil-palm-elaeis-guineensis/ 10.21894/jopr.2018.0028
institution Universiti Putra Malaysia
building UPM Library
collection Institutional Repository
continent Asia
country Malaysia
content_provider Universiti Putra Malaysia
content_source UPM Institutional Repository
url_provider http://psasir.upm.edu.my/
language English
description Thiamine (vitamin B1) is an essential microelement that is synthesised de novo by plants and microorganisms. The active form of thiamine is thiamine pyrophosphate (TPP), which plays a prominent role in plant's metabolic activity particularly as an enzymatic cofactor. In vivo analysis of thiamine in oil palm was performed where four months old oil palm seedlings were treated with 125 ml of 50 mM thiamine hydrochloride and tissue samples were collected at Day 0, 1, 2 and 3. The expression of ThiC gene fragment was analysed via quantitative real time polymerase chain reaction (qPCR) which showed an approximately five-fold decrease post-thiamine treatment. Analysis of thiamine and its derivatives via high performance liquid chromatography (HPLC) showed that the concentration of thiamine decreased, while TPP increased post-thiamine treatment. It is suggested that thiamine biosynthesis in oil palm can be effectively regulated at the physiological concentrations of the vitamin.
format Article
author Subki, Atiqah
Khairi Thaw, Siti Fariedda
Balia Yusof, Zetty Norhana
spellingShingle Subki, Atiqah
Khairi Thaw, Siti Fariedda
Balia Yusof, Zetty Norhana
Regulation of thiamine biosynthesis upon exogenous application of the vitamin in oil palm (Elaies guineensis)
author_facet Subki, Atiqah
Khairi Thaw, Siti Fariedda
Balia Yusof, Zetty Norhana
author_sort Subki, Atiqah
title Regulation of thiamine biosynthesis upon exogenous application of the vitamin in oil palm (Elaies guineensis)
title_short Regulation of thiamine biosynthesis upon exogenous application of the vitamin in oil palm (Elaies guineensis)
title_full Regulation of thiamine biosynthesis upon exogenous application of the vitamin in oil palm (Elaies guineensis)
title_fullStr Regulation of thiamine biosynthesis upon exogenous application of the vitamin in oil palm (Elaies guineensis)
title_full_unstemmed Regulation of thiamine biosynthesis upon exogenous application of the vitamin in oil palm (Elaies guineensis)
title_sort regulation of thiamine biosynthesis upon exogenous application of the vitamin in oil palm (elaies guineensis)
publisher Malaysian Palm Oil Board
publishDate 2018
url http://psasir.upm.edu.my/id/eprint/73736/1/Regulation%20of%20thiamine%20biosynthesis%20upon%20exogenous%20application%20of%20the%20vitamin%20in%20oil%20palm.pdf
http://psasir.upm.edu.my/id/eprint/73736/
http://jopr.mpob.gov.my/regulation-of-thiamine-biosynthesis-upon-exogenous-application-of-the-vitamin-in-oil-palm-elaeis-guineensis/
_version_ 1706958781505601536
score 13.211869

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