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Proteomic analysis of follicular fluid in carriers and non-carriers of the Trio allele for high ovulation rate in cattle

This study was conducted to characterise differences in follicular fluid proteins between carriers and non-carriers of a bovine allele for high ovulation rate. A total of four non-carrier and five carrier females were used in an initial study with four and six additional non-carriers and carriers re...

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Main Authors: Kamalludin, Mamat Hamidi, Guerra, Alvaro Garcia, Wiltbank, Milo C., Kirkpatrick, Brian W.
Format: Article
Language:English
Published: CSIRO Publishing 2018
Online Access:http://psasir.upm.edu.my/id/eprint/73667/1/Proteomic%20analysis%20of%20follicular%20fluid%20in%20carriers%20and%20non-carriers%20of%20the%20Trio%20allele%20for%20high%20ovulation%20rate%20in%20cattle.pdf
http://psasir.upm.edu.my/id/eprint/73667/
https://pubmed.ncbi.nlm.nih.gov/29852922/
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spelling my.upm.eprints.736672020-06-24T07:16:36Z http://psasir.upm.edu.my/id/eprint/73667/ Proteomic analysis of follicular fluid in carriers and non-carriers of the Trio allele for high ovulation rate in cattle Kamalludin, Mamat Hamidi Guerra, Alvaro Garcia Wiltbank, Milo C. Kirkpatrick, Brian W. This study was conducted to characterise differences in follicular fluid proteins between carriers and non-carriers of a bovine allele for high ovulation rate. A total of four non-carrier and five carrier females were used in an initial study with four and six additional non-carriers and carriers respectively used in a validation study. Emergence of the follicular wave was synchronised and the ovaries containing the dominant follicle(s) were extracted by ovariectomy for follicular fluid collection. A hexapeptide ligand library was used to overcome the masking effect of high-abundance proteins and to increase detection of low-abundance proteins in tandem mass spectrometry. After correcting for multiple comparisons, only two proteins, glia-derived nexin precursor (SERPINE2) and inhibin β B chain precursor (INHBB), were significantly differentially expressed (false-discovery rate <0.05). In a replicate study of analogous design differential expression was confirmed (P<0.05). Joint analysis of results from the two studies indicated that three additional proteins were consistently differentially expressed between genotypes. For three of these five, previous studies have indicated that expression is increased by transforming growth factor-β-bone morphogenetic protein signalling; their reduction in follicular fluid from carrier animals is consistent with the ~9-fold overexpression of SMAD family member 6 (SMAD6) in carriers that is inhibitory to this pathway. CSIRO Publishing 2018 Article PeerReviewed text en http://psasir.upm.edu.my/id/eprint/73667/1/Proteomic%20analysis%20of%20follicular%20fluid%20in%20carriers%20and%20non-carriers%20of%20the%20Trio%20allele%20for%20high%20ovulation%20rate%20in%20cattle.pdf Kamalludin, Mamat Hamidi and Guerra, Alvaro Garcia and Wiltbank, Milo C. and Kirkpatrick, Brian W. (2018) Proteomic analysis of follicular fluid in carriers and non-carriers of the Trio allele for high ovulation rate in cattle. Reproduction, Fertility and Development, 30 (12). 1643 - 1650. ISSN 1031-3613; ESSN: 1448-5990 https://pubmed.ncbi.nlm.nih.gov/29852922/ 10.1071/RD17252
institution Universiti Putra Malaysia
building UPM Library
collection Institutional Repository
continent Asia
country Malaysia
content_provider Universiti Putra Malaysia
content_source UPM Institutional Repository
url_provider http://psasir.upm.edu.my/
language English
description This study was conducted to characterise differences in follicular fluid proteins between carriers and non-carriers of a bovine allele for high ovulation rate. A total of four non-carrier and five carrier females were used in an initial study with four and six additional non-carriers and carriers respectively used in a validation study. Emergence of the follicular wave was synchronised and the ovaries containing the dominant follicle(s) were extracted by ovariectomy for follicular fluid collection. A hexapeptide ligand library was used to overcome the masking effect of high-abundance proteins and to increase detection of low-abundance proteins in tandem mass spectrometry. After correcting for multiple comparisons, only two proteins, glia-derived nexin precursor (SERPINE2) and inhibin β B chain precursor (INHBB), were significantly differentially expressed (false-discovery rate <0.05). In a replicate study of analogous design differential expression was confirmed (P<0.05). Joint analysis of results from the two studies indicated that three additional proteins were consistently differentially expressed between genotypes. For three of these five, previous studies have indicated that expression is increased by transforming growth factor-β-bone morphogenetic protein signalling; their reduction in follicular fluid from carrier animals is consistent with the ~9-fold overexpression of SMAD family member 6 (SMAD6) in carriers that is inhibitory to this pathway.
format Article
author Kamalludin, Mamat Hamidi
Guerra, Alvaro Garcia
Wiltbank, Milo C.
Kirkpatrick, Brian W.
spellingShingle Kamalludin, Mamat Hamidi
Guerra, Alvaro Garcia
Wiltbank, Milo C.
Kirkpatrick, Brian W.
Proteomic analysis of follicular fluid in carriers and non-carriers of the Trio allele for high ovulation rate in cattle
author_facet Kamalludin, Mamat Hamidi
Guerra, Alvaro Garcia
Wiltbank, Milo C.
Kirkpatrick, Brian W.
author_sort Kamalludin, Mamat Hamidi
title Proteomic analysis of follicular fluid in carriers and non-carriers of the Trio allele for high ovulation rate in cattle
title_short Proteomic analysis of follicular fluid in carriers and non-carriers of the Trio allele for high ovulation rate in cattle
title_full Proteomic analysis of follicular fluid in carriers and non-carriers of the Trio allele for high ovulation rate in cattle
title_fullStr Proteomic analysis of follicular fluid in carriers and non-carriers of the Trio allele for high ovulation rate in cattle
title_full_unstemmed Proteomic analysis of follicular fluid in carriers and non-carriers of the Trio allele for high ovulation rate in cattle
title_sort proteomic analysis of follicular fluid in carriers and non-carriers of the trio allele for high ovulation rate in cattle
publisher CSIRO Publishing
publishDate 2018
url http://psasir.upm.edu.my/id/eprint/73667/1/Proteomic%20analysis%20of%20follicular%20fluid%20in%20carriers%20and%20non-carriers%20of%20the%20Trio%20allele%20for%20high%20ovulation%20rate%20in%20cattle.pdf
http://psasir.upm.edu.my/id/eprint/73667/
https://pubmed.ncbi.nlm.nih.gov/29852922/
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score 13.211869

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