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Production of thermostable T1 lipase using agro-industrial waste medium formulation

Large-scale production of T1 lipase using conventional culture media is costly. To reduce the cost of production, an alternative growth medium using local resources has been developed. In this study, the growth of recombinant Escherichia coli and expression of T1 lipase were tested using different a...

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Main Authors: Mohd Nooh, Hisham, Masomian, Malihe, Salleh, Abu Bakar, Mohamad, Rosfarizan, Mohamad Ali, Mohd Shukuri, Raja Abdul Rahman, Raja Noor Zaliha
Format: Article
Language:English
Published: MDPI 2018
Online Access:http://psasir.upm.edu.my/id/eprint/73650/1/Production%20of%20thermostable%20T1%20lipase%20using%20agro-industrial%20waste%20medium%20formulation.pdf
http://psasir.upm.edu.my/id/eprint/73650/
https://www.mdpi.com/2073-4344/8/11/485
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spelling my.upm.eprints.736502020-05-10T16:59:38Z http://psasir.upm.edu.my/id/eprint/73650/ Production of thermostable T1 lipase using agro-industrial waste medium formulation Mohd Nooh, Hisham Masomian, Malihe Salleh, Abu Bakar Mohamad, Rosfarizan Mohamad Ali, Mohd Shukuri Raja Abdul Rahman, Raja Noor Zaliha Large-scale production of T1 lipase using conventional culture media is costly. To reduce the cost of production, an alternative growth medium using local resources has been developed. In this study, the growth of recombinant Escherichia coli and expression of T1 lipase were tested using different agroindustrial wastes as carbon and nitrogen sources by conventional method. Subsequently, by using central composite rotatable design (CCRD), a set of 30 experiments was generated to evaluate the effect of different parameters, including the amount of molasses (as carbon source), fish waste (as nitrogen source), NaCl, and inducer concentration on production of T1 lipase. Response surface methodology (RSM) analysis indicated that all factors had significant effects on T1 lipase production. This statistical analysis was utilised to develop a quadratic model to correlate various important variables for the growth of the recombinant strain and regulation of gene expression to the response (T1 lipase activity). Optimum conditions for T1 lipase production were observed to be 1.0 g/L of molasses, 2.29 g/L of fish waste, 3.46 g/L of NaCl, and 0.03 mM of IPTG (Isopropyl β-d-1-thiogalactopyranoside). Based on these conditions, the actual lipase activity was found to be 164.37 U/mL, which fitted well with the maximum predicted value of 172.89 U/mL. Therefore, the results demonstrated that, the statistical analysis, performed using RSM, was efficient in optimising T1 lipase production. Moreover, the optimum conditions obtained can be applied to scale up the process and minimise the cost of enzyme production. MDPI 2018 Article PeerReviewed text en http://psasir.upm.edu.my/id/eprint/73650/1/Production%20of%20thermostable%20T1%20lipase%20using%20agro-industrial%20waste%20medium%20formulation.pdf Mohd Nooh, Hisham and Masomian, Malihe and Salleh, Abu Bakar and Mohamad, Rosfarizan and Mohamad Ali, Mohd Shukuri and Raja Abdul Rahman, Raja Noor Zaliha (2018) Production of thermostable T1 lipase using agro-industrial waste medium formulation. Catalysts, 8 (11). pp. 1-18. ISSN 2073-4344; ESSN: 2073-4344 https://www.mdpi.com/2073-4344/8/11/485 10.3390/catal8110485
institution Universiti Putra Malaysia
building UPM Library
collection Institutional Repository
continent Asia
country Malaysia
content_provider Universiti Putra Malaysia
content_source UPM Institutional Repository
url_provider http://psasir.upm.edu.my/
language English
description Large-scale production of T1 lipase using conventional culture media is costly. To reduce the cost of production, an alternative growth medium using local resources has been developed. In this study, the growth of recombinant Escherichia coli and expression of T1 lipase were tested using different agroindustrial wastes as carbon and nitrogen sources by conventional method. Subsequently, by using central composite rotatable design (CCRD), a set of 30 experiments was generated to evaluate the effect of different parameters, including the amount of molasses (as carbon source), fish waste (as nitrogen source), NaCl, and inducer concentration on production of T1 lipase. Response surface methodology (RSM) analysis indicated that all factors had significant effects on T1 lipase production. This statistical analysis was utilised to develop a quadratic model to correlate various important variables for the growth of the recombinant strain and regulation of gene expression to the response (T1 lipase activity). Optimum conditions for T1 lipase production were observed to be 1.0 g/L of molasses, 2.29 g/L of fish waste, 3.46 g/L of NaCl, and 0.03 mM of IPTG (Isopropyl β-d-1-thiogalactopyranoside). Based on these conditions, the actual lipase activity was found to be 164.37 U/mL, which fitted well with the maximum predicted value of 172.89 U/mL. Therefore, the results demonstrated that, the statistical analysis, performed using RSM, was efficient in optimising T1 lipase production. Moreover, the optimum conditions obtained can be applied to scale up the process and minimise the cost of enzyme production.
format Article
author Mohd Nooh, Hisham
Masomian, Malihe
Salleh, Abu Bakar
Mohamad, Rosfarizan
Mohamad Ali, Mohd Shukuri
Raja Abdul Rahman, Raja Noor Zaliha
spellingShingle Mohd Nooh, Hisham
Masomian, Malihe
Salleh, Abu Bakar
Mohamad, Rosfarizan
Mohamad Ali, Mohd Shukuri
Raja Abdul Rahman, Raja Noor Zaliha
Production of thermostable T1 lipase using agro-industrial waste medium formulation
author_facet Mohd Nooh, Hisham
Masomian, Malihe
Salleh, Abu Bakar
Mohamad, Rosfarizan
Mohamad Ali, Mohd Shukuri
Raja Abdul Rahman, Raja Noor Zaliha
author_sort Mohd Nooh, Hisham
title Production of thermostable T1 lipase using agro-industrial waste medium formulation
title_short Production of thermostable T1 lipase using agro-industrial waste medium formulation
title_full Production of thermostable T1 lipase using agro-industrial waste medium formulation
title_fullStr Production of thermostable T1 lipase using agro-industrial waste medium formulation
title_full_unstemmed Production of thermostable T1 lipase using agro-industrial waste medium formulation
title_sort production of thermostable t1 lipase using agro-industrial waste medium formulation
publisher MDPI
publishDate 2018
url http://psasir.upm.edu.my/id/eprint/73650/1/Production%20of%20thermostable%20T1%20lipase%20using%20agro-industrial%20waste%20medium%20formulation.pdf
http://psasir.upm.edu.my/id/eprint/73650/
https://www.mdpi.com/2073-4344/8/11/485
_version_ 1669008824968675328
score 13.211869

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