Antifungal compound isolated from Catharanthus roseus L. (pink) for bological control of root rot rubber diseases
Rigidoporus microporus, Ganoderma philippii, and Phellinus noxius are root rot rubber diseases and these fungi should be kept under control with environmentally safe compounds from the plant sources. Tus, an antifungal compound isolated from Catharanthus roseus was screened for its efectiveness in c...
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Main Authors: | , , , |
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Format: | Article |
Language: | English |
Published: |
Hindawi Limited
2018
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Online Access: | http://psasir.upm.edu.my/id/eprint/73156/1/ROT.pdf http://psasir.upm.edu.my/id/eprint/73156/ https://www.hindawi.com/journals/ijac/2018/8150610/ |
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Summary: | Rigidoporus microporus, Ganoderma philippii, and Phellinus noxius are root rot rubber diseases and these fungi should be kept under control with environmentally safe compounds from the plant sources. Tus, an antifungal compound isolated from Catharanthus roseus was screened for its efectiveness in controlling the growth of these fungi. Te antifungal compound isolated from C. roseus extract was determined through thin layer chromatography (TLC) and nuclear magnetic resonance (NMR) analysis. Each C. roseus of the DCM extracts was marked as CRD1, CRD2, CRD3, CRD4, CRD5, CRD6, and CRD7, respectively. TLC results showed that all of the C. roseus extracts peaked with red colour at Rf = 0.61 at 366 nm wavelength, except for CRD7. Te CRD4 extract was found
to be the most efective against R. microporus and G. philippii with inhibition zones of 3.5 and 1.9 mm, respectively, compared to that of other extracts. Tese extracts, however, were not efective against P. noxius. Te CRD4 extract contained ursolic acid that was detected by NMR analysis and the compound could be developed as a biocontrol agent for controlling R. microporus and G. philippii. Moreover, little or no research has been done to study the effectiveness of C. roseus in controlling these fungi. |
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