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Molecular markers and phylogenetic analysis of UPMT27, a field isolate of the Malaysian fowl adenovirus associated with inclusion body hepatitis

Inclusion body hepatitis (IBH) is considered one of the re-emerging diseases of avian virus that causes economic damage worldwide. IBH is caused by different serotypes of fowl adenovirus (FAdV), and most of the FAdV cases in Malaysia are related to the serotype 8b. The objective of this study was t...

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Main Authors: Ahmed, Salisu, Abdul Razak, Mariatulqabtiah, Bejo, Mohd Hair, Omar, Abdul Rahman, Ideris, Aini, Mat Isa, Nurulfiza
Format: Article
Language:English
Published: Universiti Putra Malaysia Press 2021
Online Access:http://psasir.upm.edu.my/id/eprint/73040/1/ADENO.pdf
http://psasir.upm.edu.my/id/eprint/73040/
http://www.pertanika.upm.edu.my/pjst/browse/regular-issue?article=JST-2013-2020
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spelling my.upm.eprints.730402021-08-17T22:54:58Z http://psasir.upm.edu.my/id/eprint/73040/ Molecular markers and phylogenetic analysis of UPMT27, a field isolate of the Malaysian fowl adenovirus associated with inclusion body hepatitis Ahmed, Salisu Abdul Razak, Mariatulqabtiah Bejo, Mohd Hair Omar, Abdul Rahman Ideris, Aini Mat Isa, Nurulfiza Inclusion body hepatitis (IBH) is considered one of the re-emerging diseases of avian virus that causes economic damage worldwide. IBH is caused by different serotypes of fowl adenovirus (FAdV), and most of the FAdV cases in Malaysia are related to the serotype 8b. The objective of this study was to determine the molecular markers of UPMT27 Malaysian FAdV isolate and to identify the evolutionary relationship through the phylogenetic approach. Propagation of the isolate was made in embryonated chicken eggs and chicken embryo liver (CEL cells) before it was subjected to viral DNA extraction. Both the fiber and hexon genes of the isolate were amplified and sequenced. The sequences were aligned with the published FAdV sequences. The results showed 100% identity between UPMT27 and the previous Malaysian isolates. A phylogenetic study showed that UPMT27 was closely related to the previous Malaysian isolates. Interestingly, the substitution of the amino acids was consistent between the Malaysia isolates of both fiber protein at positions 72 (Serine –serine), 101 (Alanine -alanine), 125 (Glycine-glycine), and hexon protein 85 (Serine-serine) 160 (Glutamate- glutamate) and 205 (Alanine-alanine) respectively. It appeared that the amino acid variations were the indicators for genetic diversity. Thus, these findings provide information on the evolutionary relationship between FAdV subtypes for IBH prevention. Universiti Putra Malaysia Press 2021-01 Article PeerReviewed text en http://psasir.upm.edu.my/id/eprint/73040/1/ADENO.pdf Ahmed, Salisu and Abdul Razak, Mariatulqabtiah and Bejo, Mohd Hair and Omar, Abdul Rahman and Ideris, Aini and Mat Isa, Nurulfiza (2021) Molecular markers and phylogenetic analysis of UPMT27, a field isolate of the Malaysian fowl adenovirus associated with inclusion body hepatitis. Pertanika Journal of Science and Technology, 29 (1). pp. 547-563. ISSN 0128-7680; ESSN: 2231-8526 http://www.pertanika.upm.edu.my/pjst/browse/regular-issue?article=JST-2013-2020 10.47836/pjst.29.1.29
institution Universiti Putra Malaysia
building UPM Library
collection Institutional Repository
continent Asia
country Malaysia
content_provider Universiti Putra Malaysia
content_source UPM Institutional Repository
url_provider http://psasir.upm.edu.my/
language English
description Inclusion body hepatitis (IBH) is considered one of the re-emerging diseases of avian virus that causes economic damage worldwide. IBH is caused by different serotypes of fowl adenovirus (FAdV), and most of the FAdV cases in Malaysia are related to the serotype 8b. The objective of this study was to determine the molecular markers of UPMT27 Malaysian FAdV isolate and to identify the evolutionary relationship through the phylogenetic approach. Propagation of the isolate was made in embryonated chicken eggs and chicken embryo liver (CEL cells) before it was subjected to viral DNA extraction. Both the fiber and hexon genes of the isolate were amplified and sequenced. The sequences were aligned with the published FAdV sequences. The results showed 100% identity between UPMT27 and the previous Malaysian isolates. A phylogenetic study showed that UPMT27 was closely related to the previous Malaysian isolates. Interestingly, the substitution of the amino acids was consistent between the Malaysia isolates of both fiber protein at positions 72 (Serine –serine), 101 (Alanine -alanine), 125 (Glycine-glycine), and hexon protein 85 (Serine-serine) 160 (Glutamate- glutamate) and 205 (Alanine-alanine) respectively. It appeared that the amino acid variations were the indicators for genetic diversity. Thus, these findings provide information on the evolutionary relationship between FAdV subtypes for IBH prevention.
format Article
author Ahmed, Salisu
Abdul Razak, Mariatulqabtiah
Bejo, Mohd Hair
Omar, Abdul Rahman
Ideris, Aini
Mat Isa, Nurulfiza
spellingShingle Ahmed, Salisu
Abdul Razak, Mariatulqabtiah
Bejo, Mohd Hair
Omar, Abdul Rahman
Ideris, Aini
Mat Isa, Nurulfiza
Molecular markers and phylogenetic analysis of UPMT27, a field isolate of the Malaysian fowl adenovirus associated with inclusion body hepatitis
author_facet Ahmed, Salisu
Abdul Razak, Mariatulqabtiah
Bejo, Mohd Hair
Omar, Abdul Rahman
Ideris, Aini
Mat Isa, Nurulfiza
author_sort Ahmed, Salisu
title Molecular markers and phylogenetic analysis of UPMT27, a field isolate of the Malaysian fowl adenovirus associated with inclusion body hepatitis
title_short Molecular markers and phylogenetic analysis of UPMT27, a field isolate of the Malaysian fowl adenovirus associated with inclusion body hepatitis
title_full Molecular markers and phylogenetic analysis of UPMT27, a field isolate of the Malaysian fowl adenovirus associated with inclusion body hepatitis
title_fullStr Molecular markers and phylogenetic analysis of UPMT27, a field isolate of the Malaysian fowl adenovirus associated with inclusion body hepatitis
title_full_unstemmed Molecular markers and phylogenetic analysis of UPMT27, a field isolate of the Malaysian fowl adenovirus associated with inclusion body hepatitis
title_sort molecular markers and phylogenetic analysis of upmt27, a field isolate of the malaysian fowl adenovirus associated with inclusion body hepatitis
publisher Universiti Putra Malaysia Press
publishDate 2021
url http://psasir.upm.edu.my/id/eprint/73040/1/ADENO.pdf
http://psasir.upm.edu.my/id/eprint/73040/
http://www.pertanika.upm.edu.my/pjst/browse/regular-issue?article=JST-2013-2020
_version_ 1709668977067687936
score 13.211869

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