Staff View: GCTTCA as a novel motif for regulating mesocarp-specific expression of the oil palm (Elaeis guineensis Jacq.) stearoyl-ACP desaturase gene

GCTTCA as a novel motif for regulating mesocarp-specific expression of the oil palm (Elaeis guineensis Jacq.) stearoyl-ACP desaturase gene

Two key fatty acid biosynthetic genes, stearoyl-ACP desaturase (SAD1), and acyl-carrier protein (ACP3) in Elaeis guineensis (oil palm) showed high level of expression during the period of oil synthesis in the mesocarp [12–19 weeks after anthesis (w.a.a.)] and kernel (12–15 w.a.a.). Both genes are ex...

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Main Authors:	Abu Hanifiah, Farah Hanan, Abdullah, Siti Nor Akmar, Othman, Ashida, Shaharuddin, Noor Azmi, Mohd Saud, Halimi, Hasnulhadi, Hasnul Abdul Hakim, Munusamy, Umaiyal
Format:	Article
Language:	English
Published:	Springer 2018
Online Access:	http://psasir.upm.edu.my/id/eprint/72819/1/GCTTCA%20as%20a%20novel%20motif%20.pdf http://psasir.upm.edu.my/id/eprint/72819/ https://link.springer.com/article/10.1007/s00299-018-2300-y
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spelling	my.upm.eprints.728192021-03-11T03:51:03Z http://psasir.upm.edu.my/id/eprint/72819/ GCTTCA as a novel motif for regulating mesocarp-specific expression of the oil palm (Elaeis guineensis Jacq.) stearoyl-ACP desaturase gene Abu Hanifiah, Farah Hanan Abdullah, Siti Nor Akmar Othman, Ashida Shaharuddin, Noor Azmi Mohd Saud, Halimi Hasnulhadi, Hasnul Abdul Hakim Munusamy, Umaiyal Two key fatty acid biosynthetic genes, stearoyl-ACP desaturase (SAD1), and acyl-carrier protein (ACP3) in Elaeis guineensis (oil palm) showed high level of expression during the period of oil synthesis in the mesocarp [12–19 weeks after anthesis (w.a.a.)] and kernel (12–15 w.a.a.). Both genes are expressed in spear leaves at much lower levels and the expression increased by 1.5-fold to 2.5-fold following treatments with ethylene and abscisic acid (ABA). Both SAD1 and ACP3 promoters contain phytohormone-responsive, light-responsive, abiotic factors/wounding-responsive, endosperm specificity and fruit maturation/ripening regulatory motifs. The activities of the full length and six 5′ deletion fragments of the SAD1 promoter were analyzed in transiently transformed oil palm tissues by quantitative β-glucuronidase (GUS) fluorometric assay. The highest SAD1 promoter activity was observed in the mesocarp followed by kernel and the least in the leaves. GUS activity in the D3 deletion construct (− 486 to + 108) was the highest, while the D2 (− 535 to + 108) gave the lowest suggesting the presence of negative cis-acting regulatory element(s) in the deleted − 535 to − 486 (49 bp). It was found that the 49-bp region binds to the nuclear protein extract from mesocarp but not from leaves in electrophoretic mobility shift assay (EMSA). Further fine-tuned analysis of this 49-bp region using truncated DNA led to the identification of GCTTCA as a novel motif in the SAD1 promoter. Interestingly, another known fruit ripening-related motif, LECPLEACS2 (TAAAAT) was found to be required for effective binding of the novel motif to the mesocarp nuclear protein extract. Springer 2018 Article PeerReviewed text en http://psasir.upm.edu.my/id/eprint/72819/1/GCTTCA%20as%20a%20novel%20motif%20.pdf Abu Hanifiah, Farah Hanan and Abdullah, Siti Nor Akmar and Othman, Ashida and Shaharuddin, Noor Azmi and Mohd Saud, Halimi and Hasnulhadi, Hasnul Abdul Hakim and Munusamy, Umaiyal (2018) GCTTCA as a novel motif for regulating mesocarp-specific expression of the oil palm (Elaeis guineensis Jacq.) stearoyl-ACP desaturase gene. Plant Cell Reports, 37. 1127 - 1143. ISSN 0721-7714; ESSN: 1432-203X https://link.springer.com/article/10.1007/s00299-018-2300-y 10.1007/s00299-018-2300-y
institution	Universiti Putra Malaysia
building	UPM Library
collection	Institutional Repository
continent	Asia
country	Malaysia
content_provider	Universiti Putra Malaysia
content_source	UPM Institutional Repository
url_provider	http://psasir.upm.edu.my/
language	English
description	Two key fatty acid biosynthetic genes, stearoyl-ACP desaturase (SAD1), and acyl-carrier protein (ACP3) in Elaeis guineensis (oil palm) showed high level of expression during the period of oil synthesis in the mesocarp [12–19 weeks after anthesis (w.a.a.)] and kernel (12–15 w.a.a.). Both genes are expressed in spear leaves at much lower levels and the expression increased by 1.5-fold to 2.5-fold following treatments with ethylene and abscisic acid (ABA). Both SAD1 and ACP3 promoters contain phytohormone-responsive, light-responsive, abiotic factors/wounding-responsive, endosperm specificity and fruit maturation/ripening regulatory motifs. The activities of the full length and six 5′ deletion fragments of the SAD1 promoter were analyzed in transiently transformed oil palm tissues by quantitative β-glucuronidase (GUS) fluorometric assay. The highest SAD1 promoter activity was observed in the mesocarp followed by kernel and the least in the leaves. GUS activity in the D3 deletion construct (− 486 to + 108) was the highest, while the D2 (− 535 to + 108) gave the lowest suggesting the presence of negative cis-acting regulatory element(s) in the deleted − 535 to − 486 (49 bp). It was found that the 49-bp region binds to the nuclear protein extract from mesocarp but not from leaves in electrophoretic mobility shift assay (EMSA). Further fine-tuned analysis of this 49-bp region using truncated DNA led to the identification of GCTTCA as a novel motif in the SAD1 promoter. Interestingly, another known fruit ripening-related motif, LECPLEACS2 (TAAAAT) was found to be required for effective binding of the novel motif to the mesocarp nuclear protein extract.
format	Article
author	Abu Hanifiah, Farah Hanan Abdullah, Siti Nor Akmar Othman, Ashida Shaharuddin, Noor Azmi Mohd Saud, Halimi Hasnulhadi, Hasnul Abdul Hakim Munusamy, Umaiyal
spellingShingle	Abu Hanifiah, Farah Hanan Abdullah, Siti Nor Akmar Othman, Ashida Shaharuddin, Noor Azmi Mohd Saud, Halimi Hasnulhadi, Hasnul Abdul Hakim Munusamy, Umaiyal GCTTCA as a novel motif for regulating mesocarp-specific expression of the oil palm (Elaeis guineensis Jacq.) stearoyl-ACP desaturase gene
author_facet	Abu Hanifiah, Farah Hanan Abdullah, Siti Nor Akmar Othman, Ashida Shaharuddin, Noor Azmi Mohd Saud, Halimi Hasnulhadi, Hasnul Abdul Hakim Munusamy, Umaiyal
author_sort	Abu Hanifiah, Farah Hanan
title	GCTTCA as a novel motif for regulating mesocarp-specific expression of the oil palm (Elaeis guineensis Jacq.) stearoyl-ACP desaturase gene
title_short	GCTTCA as a novel motif for regulating mesocarp-specific expression of the oil palm (Elaeis guineensis Jacq.) stearoyl-ACP desaturase gene
title_full	GCTTCA as a novel motif for regulating mesocarp-specific expression of the oil palm (Elaeis guineensis Jacq.) stearoyl-ACP desaturase gene
title_fullStr	GCTTCA as a novel motif for regulating mesocarp-specific expression of the oil palm (Elaeis guineensis Jacq.) stearoyl-ACP desaturase gene
title_full_unstemmed	GCTTCA as a novel motif for regulating mesocarp-specific expression of the oil palm (Elaeis guineensis Jacq.) stearoyl-ACP desaturase gene
title_sort	gcttca as a novel motif for regulating mesocarp-specific expression of the oil palm (elaeis guineensis jacq.) stearoyl-acp desaturase gene
publisher	Springer
publishDate	2018
url	http://psasir.upm.edu.my/id/eprint/72819/1/GCTTCA%20as%20a%20novel%20motif%20.pdf http://psasir.upm.edu.my/id/eprint/72819/ https://link.springer.com/article/10.1007/s00299-018-2300-y
_version_	1695532742211534848
score	13.211869

GCTTCA as a novel motif for regulating mesocarp-specific expression of the oil palm (Elaeis guineensis Jacq.) stearoyl-ACP desaturase gene

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