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Gamma irradiated Py/PVA for GOx immobilization on tapered optical fiber for glucose biosensing

Tapered optical fiber coated with pyrrole/poly(vinyl alcohol)-glucose oxidase (Py/PVA-GOx) for glucose biosensing was successfully fabricated by radiation immobilization of GOx onto polymeric surfaces. Polymerization of pyrrole and PVA crosslinking was carried out by means of gamma irradiation. The...

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Main Authors: Idris, Sarada, Azeman, Nur Hidayah, Noor Azmy, Noor Azwen, Ratnam, Chantara Thevy, Mahdi, Mohd Adzir, A. Bakar, Ahmad Ashrif
Format: Article
Language:English
Published: Elsevier 2018
Online Access:http://psasir.upm.edu.my/id/eprint/72816/1/Gamma%20irradiated%20.pdf
http://psasir.upm.edu.my/id/eprint/72816/
https://www.sciencedirect.com/science/article/abs/pii/S0925400518312875
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spelling my.upm.eprints.728162021-03-10T11:01:37Z http://psasir.upm.edu.my/id/eprint/72816/ Gamma irradiated Py/PVA for GOx immobilization on tapered optical fiber for glucose biosensing Idris, Sarada Azeman, Nur Hidayah Noor Azmy, Noor Azwen Ratnam, Chantara Thevy Mahdi, Mohd Adzir A. Bakar, Ahmad Ashrif Tapered optical fiber coated with pyrrole/poly(vinyl alcohol)-glucose oxidase (Py/PVA-GOx) for glucose biosensing was successfully fabricated by radiation immobilization of GOx onto polymeric surfaces. Polymerization of pyrrole and PVA crosslinking was carried out by means of gamma irradiation. The nature of enzyme immobilization was studied by Fourier transform infrared spectroscopy, X-ray photoelectron spectroscopy, scanning electron microscopy and atomic force microscopy. The observation of an absorption band at 1650 cm−1 and binding energy formed at 287.5 eV confirm the occurrence of GOx immobilization on the polymer matrix. An increase in film thickness is observed after irradiation, which confirms the entrapment of GOx into the Py/PVA polymer matrix. The peak to valley roughness for the irradiated Py/PVA-GOx reveals the intermolecular interaction between the polymers and enzyme. These characteristics are linked to the enzymatic reaction of the coated optical fiber towards the glucose concentration. The kinetic property of the GOx in the irradiated Py/PVA-GOx coated fiber was studied with a very low value obtained for the Michaelis-Menten constant, which contributes to improved adhesion and immobilization on the coated fiber. The response and sensitivity of the coated optical fiber were recorded as <0.31 μW and 8.7 × 10-3 μWmM-1, respectively. A selectivity study reveals that the irradiated fiber coated with Py/PVA-GOx is highly selective towards glucose. Elsevier 2018 Article PeerReviewed text en http://psasir.upm.edu.my/id/eprint/72816/1/Gamma%20irradiated%20.pdf Idris, Sarada and Azeman, Nur Hidayah and Noor Azmy, Noor Azwen and Ratnam, Chantara Thevy and Mahdi, Mohd Adzir and A. Bakar, Ahmad Ashrif (2018) Gamma irradiated Py/PVA for GOx immobilization on tapered optical fiber for glucose biosensing. Sensors and Actuators B: Chemical, 273. 1404 - 1412. ISSN 0925-4005 https://www.sciencedirect.com/science/article/abs/pii/S0925400518312875 10.1016/j.snb.2018.07.053
institution Universiti Putra Malaysia
building UPM Library
collection Institutional Repository
continent Asia
country Malaysia
content_provider Universiti Putra Malaysia
content_source UPM Institutional Repository
url_provider http://psasir.upm.edu.my/
language English
description Tapered optical fiber coated with pyrrole/poly(vinyl alcohol)-glucose oxidase (Py/PVA-GOx) for glucose biosensing was successfully fabricated by radiation immobilization of GOx onto polymeric surfaces. Polymerization of pyrrole and PVA crosslinking was carried out by means of gamma irradiation. The nature of enzyme immobilization was studied by Fourier transform infrared spectroscopy, X-ray photoelectron spectroscopy, scanning electron microscopy and atomic force microscopy. The observation of an absorption band at 1650 cm−1 and binding energy formed at 287.5 eV confirm the occurrence of GOx immobilization on the polymer matrix. An increase in film thickness is observed after irradiation, which confirms the entrapment of GOx into the Py/PVA polymer matrix. The peak to valley roughness for the irradiated Py/PVA-GOx reveals the intermolecular interaction between the polymers and enzyme. These characteristics are linked to the enzymatic reaction of the coated optical fiber towards the glucose concentration. The kinetic property of the GOx in the irradiated Py/PVA-GOx coated fiber was studied with a very low value obtained for the Michaelis-Menten constant, which contributes to improved adhesion and immobilization on the coated fiber. The response and sensitivity of the coated optical fiber were recorded as <0.31 μW and 8.7 × 10-3 μWmM-1, respectively. A selectivity study reveals that the irradiated fiber coated with Py/PVA-GOx is highly selective towards glucose.
format Article
author Idris, Sarada
Azeman, Nur Hidayah
Noor Azmy, Noor Azwen
Ratnam, Chantara Thevy
Mahdi, Mohd Adzir
A. Bakar, Ahmad Ashrif
spellingShingle Idris, Sarada
Azeman, Nur Hidayah
Noor Azmy, Noor Azwen
Ratnam, Chantara Thevy
Mahdi, Mohd Adzir
A. Bakar, Ahmad Ashrif
Gamma irradiated Py/PVA for GOx immobilization on tapered optical fiber for glucose biosensing
author_facet Idris, Sarada
Azeman, Nur Hidayah
Noor Azmy, Noor Azwen
Ratnam, Chantara Thevy
Mahdi, Mohd Adzir
A. Bakar, Ahmad Ashrif
author_sort Idris, Sarada
title Gamma irradiated Py/PVA for GOx immobilization on tapered optical fiber for glucose biosensing
title_short Gamma irradiated Py/PVA for GOx immobilization on tapered optical fiber for glucose biosensing
title_full Gamma irradiated Py/PVA for GOx immobilization on tapered optical fiber for glucose biosensing
title_fullStr Gamma irradiated Py/PVA for GOx immobilization on tapered optical fiber for glucose biosensing
title_full_unstemmed Gamma irradiated Py/PVA for GOx immobilization on tapered optical fiber for glucose biosensing
title_sort gamma irradiated py/pva for gox immobilization on tapered optical fiber for glucose biosensing
publisher Elsevier
publishDate 2018
url http://psasir.upm.edu.my/id/eprint/72816/1/Gamma%20irradiated%20.pdf
http://psasir.upm.edu.my/id/eprint/72816/
https://www.sciencedirect.com/science/article/abs/pii/S0925400518312875
_version_ 1695532741852921856
score 13.211869

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