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Evaluation of six decontamination procedures for isolation of Mycobacterium avium complex from avian feces

Culture is considered the gold standard for definitive diagnosis of mycobacterial infections. However, consensus about the most suitable culture procedure for isolation of nontuberculous mycobacteria is lacking. The study compared the recoveries of mycobacteria after decontamination of spiked and fr...

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Main Authors: Mengal, Abdul Sattar, Zakaria, Zunita, Abu, Jalila, Abdul Aziz, Saleha, Gabriel, Rojas Ponce
Format: Article
Language:English
Published: Public Library of Science 2018
Online Access:http://psasir.upm.edu.my/id/eprint/72645/1/Evaluation%20of%20six%20decontamination%20procedures%20for%20isolation%20of%20Mycobacterium%20avium%20complex%20from%20avian%20feces.pdf
http://psasir.upm.edu.my/id/eprint/72645/
https://journals.plos.org/plosone/article?id=10.1371/journal.pone.0202034
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spelling my.upm.eprints.726452020-11-19T02:55:06Z http://psasir.upm.edu.my/id/eprint/72645/ Evaluation of six decontamination procedures for isolation of Mycobacterium avium complex from avian feces Mengal, Abdul Sattar Zakaria, Zunita Abu, Jalila Abdul Aziz, Saleha Gabriel, Rojas Ponce Culture is considered the gold standard for definitive diagnosis of mycobacterial infections. However, consensus about the most suitable culture procedure for isolation of nontuberculous mycobacteria is lacking. The study compared the recoveries of mycobacteria after decontamination of spiked and fresh avian feces with 4% sodium hydroxide (NaOH), 12% sulfuric acid (H2SO4), or 1% cetylperidinium chloride (CPC), with and without mixture of three antibiotics, namely vancomycin (VAN, 100 μg/ml), nalidixic acid (NAL, 100 μg/ml), and amphotericin B (AMB, 100 μg/ml). The antibiotic mixture was referred to as VNA. Decontamination procedures were evaluated using two (n = 2) avian fecal samples spiked with 106, 104, and 102 CFU/ml of Mycobacterium avium subsp. avium (ATCC 15769) and fresh avian feces (n = 42). M. avium subsp. avium was detected on the culture media from spiked samples (106 and 104 CFU/ml) decontaminated with NaOH, NaOH-VNA, H2SO4, and H2SO4 -VNA for 2−6 weeks. These bacteria were detected in 2–4 weeks when using CPC and CPC-VNA. M. avium subsp. avium cannot be isolated on culture media from spiked samples (102 CFU/ml) decontaminated with any decontaminating agent. Two mycobacterial isolates, namely, Mycobacterium terrae and M. engbaekii, were isolated from field samples decontaminated with NaOH and CPC-VNA. With regard to the contamination rate, the use of CPC-VNA showed lower contamination rates (5.5% and 19.0%) from spiked and field samples than those of the other methods (NaOH: 22.2% and 59.5%, NaOH-VNA: 16.7% and 21.4%, H2SO4: 11.1% and 40.5%, H2SO4-VNA: 5.5% and 21.4%, and CPC: 66.7% and 50%). In conclusion, the decontamination of fecal samples following a two-step procedure with 1% CPC and VNA can ensure high recovery rate of many mycobacteria with the lowest contamination in cultures. Public Library of Science 2018-08 Article PeerReviewed text en http://psasir.upm.edu.my/id/eprint/72645/1/Evaluation%20of%20six%20decontamination%20procedures%20for%20isolation%20of%20Mycobacterium%20avium%20complex%20from%20avian%20feces.pdf Mengal, Abdul Sattar and Zakaria, Zunita and Abu, Jalila and Abdul Aziz, Saleha and Gabriel, Rojas Ponce (2018) Evaluation of six decontamination procedures for isolation of Mycobacterium avium complex from avian feces. PLoS One, 13 (8). pp. 1-16. ISSN 1932-6203 https://journals.plos.org/plosone/article?id=10.1371/journal.pone.0202034 10.1371/journal.pone.0202034
institution Universiti Putra Malaysia
building UPM Library
collection Institutional Repository
continent Asia
country Malaysia
content_provider Universiti Putra Malaysia
content_source UPM Institutional Repository
url_provider http://psasir.upm.edu.my/
language English
description Culture is considered the gold standard for definitive diagnosis of mycobacterial infections. However, consensus about the most suitable culture procedure for isolation of nontuberculous mycobacteria is lacking. The study compared the recoveries of mycobacteria after decontamination of spiked and fresh avian feces with 4% sodium hydroxide (NaOH), 12% sulfuric acid (H2SO4), or 1% cetylperidinium chloride (CPC), with and without mixture of three antibiotics, namely vancomycin (VAN, 100 μg/ml), nalidixic acid (NAL, 100 μg/ml), and amphotericin B (AMB, 100 μg/ml). The antibiotic mixture was referred to as VNA. Decontamination procedures were evaluated using two (n = 2) avian fecal samples spiked with 106, 104, and 102 CFU/ml of Mycobacterium avium subsp. avium (ATCC 15769) and fresh avian feces (n = 42). M. avium subsp. avium was detected on the culture media from spiked samples (106 and 104 CFU/ml) decontaminated with NaOH, NaOH-VNA, H2SO4, and H2SO4 -VNA for 2−6 weeks. These bacteria were detected in 2–4 weeks when using CPC and CPC-VNA. M. avium subsp. avium cannot be isolated on culture media from spiked samples (102 CFU/ml) decontaminated with any decontaminating agent. Two mycobacterial isolates, namely, Mycobacterium terrae and M. engbaekii, were isolated from field samples decontaminated with NaOH and CPC-VNA. With regard to the contamination rate, the use of CPC-VNA showed lower contamination rates (5.5% and 19.0%) from spiked and field samples than those of the other methods (NaOH: 22.2% and 59.5%, NaOH-VNA: 16.7% and 21.4%, H2SO4: 11.1% and 40.5%, H2SO4-VNA: 5.5% and 21.4%, and CPC: 66.7% and 50%). In conclusion, the decontamination of fecal samples following a two-step procedure with 1% CPC and VNA can ensure high recovery rate of many mycobacteria with the lowest contamination in cultures.
format Article
author Mengal, Abdul Sattar
Zakaria, Zunita
Abu, Jalila
Abdul Aziz, Saleha
Gabriel, Rojas Ponce
spellingShingle Mengal, Abdul Sattar
Zakaria, Zunita
Abu, Jalila
Abdul Aziz, Saleha
Gabriel, Rojas Ponce
Evaluation of six decontamination procedures for isolation of Mycobacterium avium complex from avian feces
author_facet Mengal, Abdul Sattar
Zakaria, Zunita
Abu, Jalila
Abdul Aziz, Saleha
Gabriel, Rojas Ponce
author_sort Mengal, Abdul Sattar
title Evaluation of six decontamination procedures for isolation of Mycobacterium avium complex from avian feces
title_short Evaluation of six decontamination procedures for isolation of Mycobacterium avium complex from avian feces
title_full Evaluation of six decontamination procedures for isolation of Mycobacterium avium complex from avian feces
title_fullStr Evaluation of six decontamination procedures for isolation of Mycobacterium avium complex from avian feces
title_full_unstemmed Evaluation of six decontamination procedures for isolation of Mycobacterium avium complex from avian feces
title_sort evaluation of six decontamination procedures for isolation of mycobacterium avium complex from avian feces
publisher Public Library of Science
publishDate 2018
url http://psasir.upm.edu.my/id/eprint/72645/1/Evaluation%20of%20six%20decontamination%20procedures%20for%20isolation%20of%20Mycobacterium%20avium%20complex%20from%20avian%20feces.pdf
http://psasir.upm.edu.my/id/eprint/72645/
https://journals.plos.org/plosone/article?id=10.1371/journal.pone.0202034
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score 13.211869

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