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Evaluation of expression of OsAMT1;1 under different nitrogen concentration

The NH4+ and NO3− are N sources available for plant nutrition in most soils. The NH4+ role in plant nutrition has most likely been underestimated. This study provides basic information on the expression of OsAMT1;1 that is needed in understanding factors contributing to upland rice Nitrogen Use Effi...

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Main Authors: Mohd Amin, Adibah, Abdullah, Siti Nor Akmar, Musa, Mohamed Hanafi
Format: Article
Language:English
Published: The Malaysian Society For Biochemistry & Molecular Biology 2018
Online Access:http://psasir.upm.edu.my/id/eprint/72633/1/Evaluation%20of%20expression%20.pdf
http://psasir.upm.edu.my/id/eprint/72633/
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spelling my.upm.eprints.726332020-11-17T05:43:21Z http://psasir.upm.edu.my/id/eprint/72633/ Evaluation of expression of OsAMT1;1 under different nitrogen concentration Mohd Amin, Adibah Abdullah, Siti Nor Akmar Musa, Mohamed Hanafi The NH4+ and NO3− are N sources available for plant nutrition in most soils. The NH4+ role in plant nutrition has most likely been underestimated. This study provides basic information on the expression of OsAMT1;1 that is needed in understanding factors contributing to upland rice Nitrogen Use Efficiency (NUE) at the molecular level. The objective of this studies is to evaluate OsAMT1;1 expression on landraces with different NUE under different nitrogen level. To further understand the molecular basis of responsiveness of these two contrasting upland rice landraces to different N inputs at the N uptake level, a laboratory experiment was conducted to study how members of ammonium transporter gene family respond to different nitrogen conditions. Two upland varieties, Landrace III (high Nitrogen Use Efficiency) and Landrace I (low Nitrogen Use Efficiency) were grown in 4 nitrogen levels (0.05mM NH4NO3, 0.1mM NH4NO3, 1mM NH4NO3 and 2mM NH4NO3). Quantitative RT-PCR was used to study the expression of patterns of rice OsAMT1;1 genes. Results from this study showed that Landrace III has relatively higher expression of OsAMT1;1 compared to Landrace I for all treatments. Expression of OsAMT1;1 is clearly up-regulated in response to low-nitrogen treatment. Expression levels of OsAMT1;1 in high-NUE upland rice landrace was higher compared to low-NUE upland rice landrace. These results suggested, expression of OsAMT1;1 had certain degree of influenced in NUE of upland rice landraces. The Malaysian Society For Biochemistry & Molecular Biology 2018 Article PeerReviewed text en http://psasir.upm.edu.my/id/eprint/72633/1/Evaluation%20of%20expression%20.pdf Mohd Amin, Adibah and Abdullah, Siti Nor Akmar and Musa, Mohamed Hanafi (2018) Evaluation of expression of OsAMT1;1 under different nitrogen concentration. Malaysian Journal of Biochemistry and Molecular Biology, 21 (2). pp. 1-5. ISSN 2600-9005 https://msbmb2010.wixsite.com/mjbmb/copy-of-home-3
institution Universiti Putra Malaysia
building UPM Library
collection Institutional Repository
continent Asia
country Malaysia
content_provider Universiti Putra Malaysia
content_source UPM Institutional Repository
url_provider http://psasir.upm.edu.my/
language English
description The NH4+ and NO3− are N sources available for plant nutrition in most soils. The NH4+ role in plant nutrition has most likely been underestimated. This study provides basic information on the expression of OsAMT1;1 that is needed in understanding factors contributing to upland rice Nitrogen Use Efficiency (NUE) at the molecular level. The objective of this studies is to evaluate OsAMT1;1 expression on landraces with different NUE under different nitrogen level. To further understand the molecular basis of responsiveness of these two contrasting upland rice landraces to different N inputs at the N uptake level, a laboratory experiment was conducted to study how members of ammonium transporter gene family respond to different nitrogen conditions. Two upland varieties, Landrace III (high Nitrogen Use Efficiency) and Landrace I (low Nitrogen Use Efficiency) were grown in 4 nitrogen levels (0.05mM NH4NO3, 0.1mM NH4NO3, 1mM NH4NO3 and 2mM NH4NO3). Quantitative RT-PCR was used to study the expression of patterns of rice OsAMT1;1 genes. Results from this study showed that Landrace III has relatively higher expression of OsAMT1;1 compared to Landrace I for all treatments. Expression of OsAMT1;1 is clearly up-regulated in response to low-nitrogen treatment. Expression levels of OsAMT1;1 in high-NUE upland rice landrace was higher compared to low-NUE upland rice landrace. These results suggested, expression of OsAMT1;1 had certain degree of influenced in NUE of upland rice landraces.
format Article
author Mohd Amin, Adibah
Abdullah, Siti Nor Akmar
Musa, Mohamed Hanafi
spellingShingle Mohd Amin, Adibah
Abdullah, Siti Nor Akmar
Musa, Mohamed Hanafi
Evaluation of expression of OsAMT1;1 under different nitrogen concentration
author_facet Mohd Amin, Adibah
Abdullah, Siti Nor Akmar
Musa, Mohamed Hanafi
author_sort Mohd Amin, Adibah
title Evaluation of expression of OsAMT1;1 under different nitrogen concentration
title_short Evaluation of expression of OsAMT1;1 under different nitrogen concentration
title_full Evaluation of expression of OsAMT1;1 under different nitrogen concentration
title_fullStr Evaluation of expression of OsAMT1;1 under different nitrogen concentration
title_full_unstemmed Evaluation of expression of OsAMT1;1 under different nitrogen concentration
title_sort evaluation of expression of osamt1;1 under different nitrogen concentration
publisher The Malaysian Society For Biochemistry & Molecular Biology
publishDate 2018
url http://psasir.upm.edu.my/id/eprint/72633/1/Evaluation%20of%20expression%20.pdf
http://psasir.upm.edu.my/id/eprint/72633/
https://msbmb2010.wixsite.com/mjbmb/copy-of-home-3
_version_ 1684654743674159104
score 13.211869

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