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Effects of plant growth regulators on root culture and yeast extract elicitation on metabolite profiles of Polygonum minus

There are various secondary metabolites that have been identified in Polygonum minus Huds. or kesum plant, but the production is often very low and depending on growth stage. Therefore, elicitation and in vitro techniques have been suggested as an effective way for inducing secondary metabolites pro...

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Main Authors: Hassan, Mohd Azhar, Abdul Razak, Mariatulqabtiah, Baharom, Ahmad Hafiz, Md Sah, Muhammad Shafie, A. Rahman, Mohamad Zulkiffely
Format: Article
Language:English
Published: Faculty of Science and Technology, Universiti Kebangsaan Malaysia 2018
Online Access:http://psasir.upm.edu.my/id/eprint/72511/1/Effects%20of%20plant%20growth%20regulators%20on%20root%20culture%20.pdf
http://psasir.upm.edu.my/id/eprint/72511/
http://www.ukm.my/jsm/english_journals/vol47num12_2018/vol47num12_2018pg3085-3094.html
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spelling my.upm.eprints.725112020-10-20T09:41:21Z http://psasir.upm.edu.my/id/eprint/72511/ Effects of plant growth regulators on root culture and yeast extract elicitation on metabolite profiles of Polygonum minus Hassan, Mohd Azhar Abdul Razak, Mariatulqabtiah Baharom, Ahmad Hafiz Md Sah, Muhammad Shafie A. Rahman, Mohamad Zulkiffely There are various secondary metabolites that have been identified in Polygonum minus Huds. or kesum plant, but the production is often very low and depending on growth stage. Therefore, elicitation and in vitro techniques have been suggested as an effective way for inducing secondary metabolites production in plant. This study was conducted to determine the optimal conditions for P. minus root formation in vitro and to profile the metabolite content from P. minus root culture with and without elicitor treatment. From the root induction study, it was found that the fresh weight of induced root for nodal explant in MS liquid media supplemented with 0.5 mg/L NAA and shaken had the highest production (0.38±0.08 g) compared to other treatments including the control. The results from metabolite profile showed that the volatile compound of P. minus root produced without any elicitation contained 50.11% aliphatic (27.59% aldehide, 9.17% alkane and 13.35% others) and 19.39% sesquiterpene (β-caryophyllene, α-bergamotene, β-farnesene, α-caryophyllene dan β-curcumene) where the dodecanal compound (22.27%) and β-caryophyllene (8.09%) have the highest percentage value for aliphatic and sesquiterpene group, respectively. Moreover, elicitation of P. minus root culture using yeast extract at 100 mg/L concentration for 1 day demonstrated the ability to increase the production of secondary metabolites in many volatile compounds of kesum in vitro root including the sesquiterpene compounds compared to control treatment and other yeast extract elicitation treatments. Faculty of Science and Technology, Universiti Kebangsaan Malaysia 2018 Article PeerReviewed text en http://psasir.upm.edu.my/id/eprint/72511/1/Effects%20of%20plant%20growth%20regulators%20on%20root%20culture%20.pdf Hassan, Mohd Azhar and Abdul Razak, Mariatulqabtiah and Baharom, Ahmad Hafiz and Md Sah, Muhammad Shafie and A. Rahman, Mohamad Zulkiffely (2018) Effects of plant growth regulators on root culture and yeast extract elicitation on metabolite profiles of Polygonum minus. Sains Malaysiana, 47 (12). 3085 - 3094. ISSN 0126-6039 http://www.ukm.my/jsm/english_journals/vol47num12_2018/vol47num12_2018pg3085-3094.html 10.17576/jsm-2018-4712-19
institution Universiti Putra Malaysia
building UPM Library
collection Institutional Repository
continent Asia
country Malaysia
content_provider Universiti Putra Malaysia
content_source UPM Institutional Repository
url_provider http://psasir.upm.edu.my/
language English
description There are various secondary metabolites that have been identified in Polygonum minus Huds. or kesum plant, but the production is often very low and depending on growth stage. Therefore, elicitation and in vitro techniques have been suggested as an effective way for inducing secondary metabolites production in plant. This study was conducted to determine the optimal conditions for P. minus root formation in vitro and to profile the metabolite content from P. minus root culture with and without elicitor treatment. From the root induction study, it was found that the fresh weight of induced root for nodal explant in MS liquid media supplemented with 0.5 mg/L NAA and shaken had the highest production (0.38±0.08 g) compared to other treatments including the control. The results from metabolite profile showed that the volatile compound of P. minus root produced without any elicitation contained 50.11% aliphatic (27.59% aldehide, 9.17% alkane and 13.35% others) and 19.39% sesquiterpene (β-caryophyllene, α-bergamotene, β-farnesene, α-caryophyllene dan β-curcumene) where the dodecanal compound (22.27%) and β-caryophyllene (8.09%) have the highest percentage value for aliphatic and sesquiterpene group, respectively. Moreover, elicitation of P. minus root culture using yeast extract at 100 mg/L concentration for 1 day demonstrated the ability to increase the production of secondary metabolites in many volatile compounds of kesum in vitro root including the sesquiterpene compounds compared to control treatment and other yeast extract elicitation treatments.
format Article
author Hassan, Mohd Azhar
Abdul Razak, Mariatulqabtiah
Baharom, Ahmad Hafiz
Md Sah, Muhammad Shafie
A. Rahman, Mohamad Zulkiffely
spellingShingle Hassan, Mohd Azhar
Abdul Razak, Mariatulqabtiah
Baharom, Ahmad Hafiz
Md Sah, Muhammad Shafie
A. Rahman, Mohamad Zulkiffely
Effects of plant growth regulators on root culture and yeast extract elicitation on metabolite profiles of Polygonum minus
author_facet Hassan, Mohd Azhar
Abdul Razak, Mariatulqabtiah
Baharom, Ahmad Hafiz
Md Sah, Muhammad Shafie
A. Rahman, Mohamad Zulkiffely
author_sort Hassan, Mohd Azhar
title Effects of plant growth regulators on root culture and yeast extract elicitation on metabolite profiles of Polygonum minus
title_short Effects of plant growth regulators on root culture and yeast extract elicitation on metabolite profiles of Polygonum minus
title_full Effects of plant growth regulators on root culture and yeast extract elicitation on metabolite profiles of Polygonum minus
title_fullStr Effects of plant growth regulators on root culture and yeast extract elicitation on metabolite profiles of Polygonum minus
title_full_unstemmed Effects of plant growth regulators on root culture and yeast extract elicitation on metabolite profiles of Polygonum minus
title_sort effects of plant growth regulators on root culture and yeast extract elicitation on metabolite profiles of polygonum minus
publisher Faculty of Science and Technology, Universiti Kebangsaan Malaysia
publishDate 2018
url http://psasir.upm.edu.my/id/eprint/72511/1/Effects%20of%20plant%20growth%20regulators%20on%20root%20culture%20.pdf
http://psasir.upm.edu.my/id/eprint/72511/
http://www.ukm.my/jsm/english_journals/vol47num12_2018/vol47num12_2018pg3085-3094.html
_version_ 1681490794955931648
score 13.211869

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