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Development of an optical sensor based on surface plasmon resonance phenomenon for diagnosis of dengue virus E-protein

Application of surface plasmon resonance (SPR) optical sensor in the diagnosis of dengue virus (DENV) emerged over recent years. Immobilized monoclonal antibody (IgM) on gold/Fe-MPA-NCC-CTAB/EDC-NHS thin film was prepared using a spin coating technique. DENV E-protein can be detected by measuring th...

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Main Authors: Sheh Omar, Nur Alia, Yap, Wing Fen, Abdullah, Jaafar, Che Engku Chik, Che Engku Noramalina, Mahdi, Mohd Adzir
Format: Article
Language:English
Published: Elsevier 2018
Online Access:http://psasir.upm.edu.my/id/eprint/72252/1/Development%20of%20an%20optical%20sensor%20based%20on%20surface%20plasmon%20resonance%20phenomenon%20for%20diagnosis%20of%20dengue%20virus%20E-protein.pdf
http://psasir.upm.edu.my/id/eprint/72252/
https://www.sciencedirect.com/science/article/pii/S2214180417301757
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spelling my.upm.eprints.722522020-04-26T05:39:45Z http://psasir.upm.edu.my/id/eprint/72252/ Development of an optical sensor based on surface plasmon resonance phenomenon for diagnosis of dengue virus E-protein Sheh Omar, Nur Alia Yap, Wing Fen Abdullah, Jaafar Che Engku Chik, Che Engku Noramalina Mahdi, Mohd Adzir Application of surface plasmon resonance (SPR) optical sensor in the diagnosis of dengue virus (DENV) emerged over recent years. Immobilized monoclonal antibody (IgM) on gold/Fe-MPA-NCC-CTAB/EDC-NHS thin film was prepared using a spin coating technique. DENV E-protein can be detected by measuring the SPR signal when IgM immobilized gold/Fe-MPA-NCC-CTAB/EDC-NHS thin film is exposed to the DENV E-protein solution, by varying the concentration ranging from 0.0001 nM to 10 nM. A linear relationship between the shift of SPR angle and concentration of DENV E-protein up to 0.01 nM, with sensitivity of 39.96° nM−1 has been observed. The surface morphology of sensor chip was also recorded using atomic force microscopy to confirm the presence of bound DENV E-protein into IgM surface. Elsevier 2018-09 Article PeerReviewed text en http://psasir.upm.edu.my/id/eprint/72252/1/Development%20of%20an%20optical%20sensor%20based%20on%20surface%20plasmon%20resonance%20phenomenon%20for%20diagnosis%20of%20dengue%20virus%20E-protein.pdf Sheh Omar, Nur Alia and Yap, Wing Fen and Abdullah, Jaafar and Che Engku Chik, Che Engku Noramalina and Mahdi, Mohd Adzir (2018) Development of an optical sensor based on surface plasmon resonance phenomenon for diagnosis of dengue virus E-protein. Sensing and Bio-Sensing Research, 20. 16 - 21. ISSN 2214-1804 https://www.sciencedirect.com/science/article/pii/S2214180417301757 10.1016/j.sbsr.2018.06.001
institution Universiti Putra Malaysia
building UPM Library
collection Institutional Repository
continent Asia
country Malaysia
content_provider Universiti Putra Malaysia
content_source UPM Institutional Repository
url_provider http://psasir.upm.edu.my/
language English
description Application of surface plasmon resonance (SPR) optical sensor in the diagnosis of dengue virus (DENV) emerged over recent years. Immobilized monoclonal antibody (IgM) on gold/Fe-MPA-NCC-CTAB/EDC-NHS thin film was prepared using a spin coating technique. DENV E-protein can be detected by measuring the SPR signal when IgM immobilized gold/Fe-MPA-NCC-CTAB/EDC-NHS thin film is exposed to the DENV E-protein solution, by varying the concentration ranging from 0.0001 nM to 10 nM. A linear relationship between the shift of SPR angle and concentration of DENV E-protein up to 0.01 nM, with sensitivity of 39.96° nM−1 has been observed. The surface morphology of sensor chip was also recorded using atomic force microscopy to confirm the presence of bound DENV E-protein into IgM surface.
format Article
author Sheh Omar, Nur Alia
Yap, Wing Fen
Abdullah, Jaafar
Che Engku Chik, Che Engku Noramalina
Mahdi, Mohd Adzir
spellingShingle Sheh Omar, Nur Alia
Yap, Wing Fen
Abdullah, Jaafar
Che Engku Chik, Che Engku Noramalina
Mahdi, Mohd Adzir
Development of an optical sensor based on surface plasmon resonance phenomenon for diagnosis of dengue virus E-protein
author_facet Sheh Omar, Nur Alia
Yap, Wing Fen
Abdullah, Jaafar
Che Engku Chik, Che Engku Noramalina
Mahdi, Mohd Adzir
author_sort Sheh Omar, Nur Alia
title Development of an optical sensor based on surface plasmon resonance phenomenon for diagnosis of dengue virus E-protein
title_short Development of an optical sensor based on surface plasmon resonance phenomenon for diagnosis of dengue virus E-protein
title_full Development of an optical sensor based on surface plasmon resonance phenomenon for diagnosis of dengue virus E-protein
title_fullStr Development of an optical sensor based on surface plasmon resonance phenomenon for diagnosis of dengue virus E-protein
title_full_unstemmed Development of an optical sensor based on surface plasmon resonance phenomenon for diagnosis of dengue virus E-protein
title_sort development of an optical sensor based on surface plasmon resonance phenomenon for diagnosis of dengue virus e-protein
publisher Elsevier
publishDate 2018
url http://psasir.upm.edu.my/id/eprint/72252/1/Development%20of%20an%20optical%20sensor%20based%20on%20surface%20plasmon%20resonance%20phenomenon%20for%20diagnosis%20of%20dengue%20virus%20E-protein.pdf
http://psasir.upm.edu.my/id/eprint/72252/
https://www.sciencedirect.com/science/article/pii/S2214180417301757
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score 13.211869

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