Cover Image

Development of an electrochemical DNA biosensor to detect a foodborne pathogen

Vibrio parahaemolyticus (V. parahaemolyticus) is a common foodborne pathogen that contributes to a large proportion of public health problems globally, significantly affecting the rate of human mortality and morbidity. Conventional methods for the detection of V. parahaemolyticus such as culture-bas...

Full description

Saved in:
Bibliographic Details
Main Authors: Nordin, Noordiana, Yusof, Nor Azah, Radu, Son, Hushiarian, Roozbeh
Format: Article
Language:English
Published: Journal of Visualized Experiments 2018
Online Access:http://psasir.upm.edu.my/id/eprint/72251/1/Development%20of%20an%20electrochemical%20DNA%20biosensor%20to%20detect%20a%20foodborne%20pathogen.pdf
http://psasir.upm.edu.my/id/eprint/72251/
https://www.jove.com/video/56585/development-an-electrochemical-dna-biosensor-to-detect-foodborne
Tags: Add Tag
No Tags, Be the first to tag this record!
id my.upm.eprints.72251
record_format eprints
spelling my.upm.eprints.722512020-04-26T04:29:20Z http://psasir.upm.edu.my/id/eprint/72251/ Development of an electrochemical DNA biosensor to detect a foodborne pathogen Nordin, Noordiana Yusof, Nor Azah Radu, Son Hushiarian, Roozbeh Vibrio parahaemolyticus (V. parahaemolyticus) is a common foodborne pathogen that contributes to a large proportion of public health problems globally, significantly affecting the rate of human mortality and morbidity. Conventional methods for the detection of V. parahaemolyticus such as culture-based methods, immunological assays, and molecular-based methods require complicated sample handling and are time-consuming, tedious, and costly. Recently, biosensors have proven to be a promising and comprehensive detection method with the advantages of fast detection, cost-effectiveness, and practicality. This research focuses on developing a rapid method of detecting V. parahaemolyticus with high selectivity and sensitivity using the principles of DNA hybridization. In the work, characterization of synthesized polylactic acid-stabilized gold nanoparticles (PLA-AuNPs) was achieved using X-ray Diffraction (XRD), Ultraviolet-visible Spectroscopy (UV-Vis), Transmission Electron Microscopy (TEM), Field-emission Scanning Electron Microscopy (FESEM), and Cyclic Voltammetry (CV). We also carried out further testing of stability, sensitivity, and reproducibility of the PLA-AuNPs. We found that the PLA-AuNPs formed a sound structure of stabilized nanoparticles in aqueous solution. We also observed that the sensitivity improved as a result of the smaller charge transfer resistance (Rct) value and an increase of active surface area (0.41 cm2). The development of our DNA biosensor was based on modification of a screen-printed carbon electrode (SPCE) with PLA-AuNPs and using methylene blue (MB) as the redox indicator. We assessed the immobilization and hybridization events by differential pulse voltammetry (DPV). We found that complementary, non-complementary, and mismatched oligonucleotides were specifically distinguished by the fabricated biosensor. It also showed reliably sensitive detection in cross-reactivity studies against various food-borne pathogens and in the identification of V. parahaemolyticus in fresh cockles. Journal of Visualized Experiments 2018-06 Article PeerReviewed text en http://psasir.upm.edu.my/id/eprint/72251/1/Development%20of%20an%20electrochemical%20DNA%20biosensor%20to%20detect%20a%20foodborne%20pathogen.pdf Nordin, Noordiana and Yusof, Nor Azah and Radu, Son and Hushiarian, Roozbeh (2018) Development of an electrochemical DNA biosensor to detect a foodborne pathogen. Journal of Visualized Experiments (136). pp. 1-17. ISSN 1940-087X https://www.jove.com/video/56585/development-an-electrochemical-dna-biosensor-to-detect-foodborne 10.3791/56585
institution Universiti Putra Malaysia
building UPM Library
collection Institutional Repository
continent Asia
country Malaysia
content_provider Universiti Putra Malaysia
content_source UPM Institutional Repository
url_provider http://psasir.upm.edu.my/
language English
description Vibrio parahaemolyticus (V. parahaemolyticus) is a common foodborne pathogen that contributes to a large proportion of public health problems globally, significantly affecting the rate of human mortality and morbidity. Conventional methods for the detection of V. parahaemolyticus such as culture-based methods, immunological assays, and molecular-based methods require complicated sample handling and are time-consuming, tedious, and costly. Recently, biosensors have proven to be a promising and comprehensive detection method with the advantages of fast detection, cost-effectiveness, and practicality. This research focuses on developing a rapid method of detecting V. parahaemolyticus with high selectivity and sensitivity using the principles of DNA hybridization. In the work, characterization of synthesized polylactic acid-stabilized gold nanoparticles (PLA-AuNPs) was achieved using X-ray Diffraction (XRD), Ultraviolet-visible Spectroscopy (UV-Vis), Transmission Electron Microscopy (TEM), Field-emission Scanning Electron Microscopy (FESEM), and Cyclic Voltammetry (CV). We also carried out further testing of stability, sensitivity, and reproducibility of the PLA-AuNPs. We found that the PLA-AuNPs formed a sound structure of stabilized nanoparticles in aqueous solution. We also observed that the sensitivity improved as a result of the smaller charge transfer resistance (Rct) value and an increase of active surface area (0.41 cm2). The development of our DNA biosensor was based on modification of a screen-printed carbon electrode (SPCE) with PLA-AuNPs and using methylene blue (MB) as the redox indicator. We assessed the immobilization and hybridization events by differential pulse voltammetry (DPV). We found that complementary, non-complementary, and mismatched oligonucleotides were specifically distinguished by the fabricated biosensor. It also showed reliably sensitive detection in cross-reactivity studies against various food-borne pathogens and in the identification of V. parahaemolyticus in fresh cockles.
format Article
author Nordin, Noordiana
Yusof, Nor Azah
Radu, Son
Hushiarian, Roozbeh
spellingShingle Nordin, Noordiana
Yusof, Nor Azah
Radu, Son
Hushiarian, Roozbeh
Development of an electrochemical DNA biosensor to detect a foodborne pathogen
author_facet Nordin, Noordiana
Yusof, Nor Azah
Radu, Son
Hushiarian, Roozbeh
author_sort Nordin, Noordiana
title Development of an electrochemical DNA biosensor to detect a foodborne pathogen
title_short Development of an electrochemical DNA biosensor to detect a foodborne pathogen
title_full Development of an electrochemical DNA biosensor to detect a foodborne pathogen
title_fullStr Development of an electrochemical DNA biosensor to detect a foodborne pathogen
title_full_unstemmed Development of an electrochemical DNA biosensor to detect a foodborne pathogen
title_sort development of an electrochemical dna biosensor to detect a foodborne pathogen
publisher Journal of Visualized Experiments
publishDate 2018
url http://psasir.upm.edu.my/id/eprint/72251/1/Development%20of%20an%20electrochemical%20DNA%20biosensor%20to%20detect%20a%20foodborne%20pathogen.pdf
http://psasir.upm.edu.my/id/eprint/72251/
https://www.jove.com/video/56585/development-an-electrochemical-dna-biosensor-to-detect-foodborne
_version_ 1665895993830277120
score 13.211869

Similar Items