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CUPRAC assay-guided profiling of antioxidant compounds in methanolic extract of Lentinus squarrosulus mycelium

A cupric reducing antioxidant capacity (CUPRAC)-guided purification approach was performed on a methanol extract of Lentinus squarrosulus (LsqMeOH) by using reversed phase-high performance liquid chromatography. Using reversed phase-high performance liquid chromatography, three fractions were separa...

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Main Authors: Abdullah, Sumaiyah, Abdullah, Noorlidah, Sabaratnam, Vikineswary, Yap, Ken Choy
Format: Article
Language:English
Published: Biotechnical Faculty, University of Ljubljana 2018
Online Access:http://psasir.upm.edu.my/id/eprint/72153/1/CUPRAC%20assay-guided%20profiling%20of%20antioxidant%20compounds.pdf
http://psasir.upm.edu.my/id/eprint/72153/
http://ojs.aas.bf.uni-lj.si/index.php/AAS/article/view/600
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spelling my.upm.eprints.721532020-03-04T08:25:57Z http://psasir.upm.edu.my/id/eprint/72153/ CUPRAC assay-guided profiling of antioxidant compounds in methanolic extract of Lentinus squarrosulus mycelium Abdullah, Sumaiyah Abdullah, Noorlidah Sabaratnam, Vikineswary Yap, Ken Choy A cupric reducing antioxidant capacity (CUPRAC)-guided purification approach was performed on a methanol extract of Lentinus squarrosulus (LsqMeOH) by using reversed phase-high performance liquid chromatography. Using reversed phase-high performance liquid chromatography, three fractions were separated arbitrarily named FR1, FR2 and FR3. Results showed that FR2 exhibited the highest antioxidant activity in CUPRAC assay (A450, 0.86) but not significantly different from LsqMeOH (A450, 0.84). FR1 and FR3 showed much lower absorbance, with values (A450, 0.21) and (A450, 0.36) respectively at 1 mg ml-1. The most active fraction (F3) was further subjected to LC-MS/MS to obtain its detailed chemical profile. Uridine, ganoderic acid derivative, and flavonoids were the first time being found in L. squarrosulus antioxidative fractions. The present results indicate that the fraction extracts of L. squarrosulus possess antioxidant properties and can be used as free radical inhibitors. Therefore, this research suggested the potentials of L. squarrosulus as a source of antioxidant extract to be used in food industries (functional food). Biotechnical Faculty, University of Ljubljana 2018 Article PeerReviewed text en http://psasir.upm.edu.my/id/eprint/72153/1/CUPRAC%20assay-guided%20profiling%20of%20antioxidant%20compounds.pdf Abdullah, Sumaiyah and Abdullah, Noorlidah and Sabaratnam, Vikineswary and Yap, Ken Choy (2018) CUPRAC assay-guided profiling of antioxidant compounds in methanolic extract of Lentinus squarrosulus mycelium. Acta Agriculturae Slovenica, 111 (1). 101 - 109. ISSN 1581-9175; ESSN: 1854-1941 http://ojs.aas.bf.uni-lj.si/index.php/AAS/article/view/600 10.14720/aas.2018.111.1.10
institution Universiti Putra Malaysia
building UPM Library
collection Institutional Repository
continent Asia
country Malaysia
content_provider Universiti Putra Malaysia
content_source UPM Institutional Repository
url_provider http://psasir.upm.edu.my/
language English
description A cupric reducing antioxidant capacity (CUPRAC)-guided purification approach was performed on a methanol extract of Lentinus squarrosulus (LsqMeOH) by using reversed phase-high performance liquid chromatography. Using reversed phase-high performance liquid chromatography, three fractions were separated arbitrarily named FR1, FR2 and FR3. Results showed that FR2 exhibited the highest antioxidant activity in CUPRAC assay (A450, 0.86) but not significantly different from LsqMeOH (A450, 0.84). FR1 and FR3 showed much lower absorbance, with values (A450, 0.21) and (A450, 0.36) respectively at 1 mg ml-1. The most active fraction (F3) was further subjected to LC-MS/MS to obtain its detailed chemical profile. Uridine, ganoderic acid derivative, and flavonoids were the first time being found in L. squarrosulus antioxidative fractions. The present results indicate that the fraction extracts of L. squarrosulus possess antioxidant properties and can be used as free radical inhibitors. Therefore, this research suggested the potentials of L. squarrosulus as a source of antioxidant extract to be used in food industries (functional food).
format Article
author Abdullah, Sumaiyah
Abdullah, Noorlidah
Sabaratnam, Vikineswary
Yap, Ken Choy
spellingShingle Abdullah, Sumaiyah
Abdullah, Noorlidah
Sabaratnam, Vikineswary
Yap, Ken Choy
CUPRAC assay-guided profiling of antioxidant compounds in methanolic extract of Lentinus squarrosulus mycelium
author_facet Abdullah, Sumaiyah
Abdullah, Noorlidah
Sabaratnam, Vikineswary
Yap, Ken Choy
author_sort Abdullah, Sumaiyah
title CUPRAC assay-guided profiling of antioxidant compounds in methanolic extract of Lentinus squarrosulus mycelium
title_short CUPRAC assay-guided profiling of antioxidant compounds in methanolic extract of Lentinus squarrosulus mycelium
title_full CUPRAC assay-guided profiling of antioxidant compounds in methanolic extract of Lentinus squarrosulus mycelium
title_fullStr CUPRAC assay-guided profiling of antioxidant compounds in methanolic extract of Lentinus squarrosulus mycelium
title_full_unstemmed CUPRAC assay-guided profiling of antioxidant compounds in methanolic extract of Lentinus squarrosulus mycelium
title_sort cuprac assay-guided profiling of antioxidant compounds in methanolic extract of lentinus squarrosulus mycelium
publisher Biotechnical Faculty, University of Ljubljana
publishDate 2018
url http://psasir.upm.edu.my/id/eprint/72153/1/CUPRAC%20assay-guided%20profiling%20of%20antioxidant%20compounds.pdf
http://psasir.upm.edu.my/id/eprint/72153/
http://ojs.aas.bf.uni-lj.si/index.php/AAS/article/view/600
_version_ 1662756481486815232
score 13.211869

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