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High performance enzymatic synthesis of oleyl oleate using immobilised lipase from Candida antartica

High performance enzymatic synthesis of oleyl oleate, a liquid wax ester was carried out by lipase-catalysed esterification of oleic acid and oleyl alcohol. Various reaction parameters were optimised to obtain high yield of oleyl oleate. The optimum condition to produce oleyl oleate was reaction tim...

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Main Authors: Mat Radzi, Salina, Basri, Mahiran, Salleh, Abu Bakar, Ariff, Arbakariya, Mohammad, Rosfarizan, Abdul Rahman, Mohd Basyaruddin, Raja Abdul Rahman, Raja Noor Zaliha
Format: Article
Language:English
Published: Pontificia Universidad Católica de Valparaíso 2005
Online Access:http://psasir.upm.edu.my/id/eprint/7056/1/High%20performance%20enzymatic%20synthesis%20of%20oleyl%20oleate%20using%20immobilised%20lipase%20from%20Candida%20antartica.pdf
http://psasir.upm.edu.my/id/eprint/7056/
http://www.ejbiotechnology.info/index.php/ejbiotechnology/article/view/v8n3-4
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spelling my.upm.eprints.70562016-09-28T02:58:31Z http://psasir.upm.edu.my/id/eprint/7056/ High performance enzymatic synthesis of oleyl oleate using immobilised lipase from Candida antartica Mat Radzi, Salina Basri, Mahiran Salleh, Abu Bakar Ariff, Arbakariya Mohammad, Rosfarizan Abdul Rahman, Mohd Basyaruddin Raja Abdul Rahman, Raja Noor Zaliha High performance enzymatic synthesis of oleyl oleate, a liquid wax ester was carried out by lipase-catalysed esterification of oleic acid and oleyl alcohol. Various reaction parameters were optimised to obtain high yield of oleyl oleate. The optimum condition to produce oleyl oleate was reaction time; 5 min, organic solvents of log P ≥ 3.5, temperature; 40-50ºC, amount of enzyme; 0.2-0.4 g and molar ratio of oleyl alcohol to oleic acid; 2:1. The operational stability of enzyme was maintained at >90% yield up to 9 cycles. Analysis of the yield of the product showed that at optimum conditions, >95% liquid wax esters were produced. Pontificia Universidad Católica de Valparaíso 2005-12-15 Article PeerReviewed application/pdf en http://psasir.upm.edu.my/id/eprint/7056/1/High%20performance%20enzymatic%20synthesis%20of%20oleyl%20oleate%20using%20immobilised%20lipase%20from%20Candida%20antartica.pdf Mat Radzi, Salina and Basri, Mahiran and Salleh, Abu Bakar and Ariff, Arbakariya and Mohammad, Rosfarizan and Abdul Rahman, Mohd Basyaruddin and Raja Abdul Rahman, Raja Noor Zaliha (2005) High performance enzymatic synthesis of oleyl oleate using immobilised lipase from Candida antartica. Electronic Journal of Biotechnology, 8 (3). pp. 291-298. ISSN 0717-3458 http://www.ejbiotechnology.info/index.php/ejbiotechnology/article/view/v8n3-4 10.2225/vol8-issue3-fulltext-4
institution Universiti Putra Malaysia
building UPM Library
collection Institutional Repository
continent Asia
country Malaysia
content_provider Universiti Putra Malaysia
content_source UPM Institutional Repository
url_provider http://psasir.upm.edu.my/
language English
description High performance enzymatic synthesis of oleyl oleate, a liquid wax ester was carried out by lipase-catalysed esterification of oleic acid and oleyl alcohol. Various reaction parameters were optimised to obtain high yield of oleyl oleate. The optimum condition to produce oleyl oleate was reaction time; 5 min, organic solvents of log P ≥ 3.5, temperature; 40-50ºC, amount of enzyme; 0.2-0.4 g and molar ratio of oleyl alcohol to oleic acid; 2:1. The operational stability of enzyme was maintained at >90% yield up to 9 cycles. Analysis of the yield of the product showed that at optimum conditions, >95% liquid wax esters were produced.
format Article
author Mat Radzi, Salina
Basri, Mahiran
Salleh, Abu Bakar
Ariff, Arbakariya
Mohammad, Rosfarizan
Abdul Rahman, Mohd Basyaruddin
Raja Abdul Rahman, Raja Noor Zaliha
spellingShingle Mat Radzi, Salina
Basri, Mahiran
Salleh, Abu Bakar
Ariff, Arbakariya
Mohammad, Rosfarizan
Abdul Rahman, Mohd Basyaruddin
Raja Abdul Rahman, Raja Noor Zaliha
High performance enzymatic synthesis of oleyl oleate using immobilised lipase from Candida antartica
author_facet Mat Radzi, Salina
Basri, Mahiran
Salleh, Abu Bakar
Ariff, Arbakariya
Mohammad, Rosfarizan
Abdul Rahman, Mohd Basyaruddin
Raja Abdul Rahman, Raja Noor Zaliha
author_sort Mat Radzi, Salina
title High performance enzymatic synthesis of oleyl oleate using immobilised lipase from Candida antartica
title_short High performance enzymatic synthesis of oleyl oleate using immobilised lipase from Candida antartica
title_full High performance enzymatic synthesis of oleyl oleate using immobilised lipase from Candida antartica
title_fullStr High performance enzymatic synthesis of oleyl oleate using immobilised lipase from Candida antartica
title_full_unstemmed High performance enzymatic synthesis of oleyl oleate using immobilised lipase from Candida antartica
title_sort high performance enzymatic synthesis of oleyl oleate using immobilised lipase from candida antartica
publisher Pontificia Universidad Católica de Valparaíso
publishDate 2005
url http://psasir.upm.edu.my/id/eprint/7056/1/High%20performance%20enzymatic%20synthesis%20of%20oleyl%20oleate%20using%20immobilised%20lipase%20from%20Candida%20antartica.pdf
http://psasir.upm.edu.my/id/eprint/7056/
http://www.ejbiotechnology.info/index.php/ejbiotechnology/article/view/v8n3-4
_version_ 1643823610008174592
score 13.211869

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