Cover Image

Biological control of Pyricularia oryzae, using fungal metabolites

Rice blast caused by fungus Pyricularia oryzea is one of the most devastating among rice diseases. The disease has been classified as one of the most serious diseases that influence the production of rice since rice fields are seriously damaged, thereby affecting yield and quality. To decrease the u...

Full description

Saved in:
Bibliographic Details
Main Author: Idan, Ali Abdulameer
Format: Thesis
Language:English
Published: 2017
Online Access:http://psasir.upm.edu.my/id/eprint/70352/1/FP%202017%2025%20IR.pdf
http://psasir.upm.edu.my/id/eprint/70352/
Tags: Add Tag
No Tags, Be the first to tag this record!
id my.upm.eprints.70352
record_format eprints
spelling my.upm.eprints.703522019-09-20T02:51:15Z http://psasir.upm.edu.my/id/eprint/70352/ Biological control of Pyricularia oryzae, using fungal metabolites Idan, Ali Abdulameer Rice blast caused by fungus Pyricularia oryzea is one of the most devastating among rice diseases. The disease has been classified as one of the most serious diseases that influence the production of rice since rice fields are seriously damaged, thereby affecting yield and quality. To decrease the use of pesticides, biological control is considered as an alternative sustainable control. Aspergillus spp. has been widely found to be useful in biotechnology as a bio control agent. They have a high ability in the production of secondary metabolites. Aspergillus spp. was obtained from the rice field soil located in Selangor, Tanjung Karang, Peninsular Malaysia. Isolates UPMZ01 and UPMZ02 were screened against blast pathogen by applying dual culture technique. The antagonism of isolate UPMZ01 was (81.326%) while UPMZ02 gave (70.9%) of inhibition percentage against P. oryzae. Culture filtrate was also used to screen the inhibitory effect against the pathogen. The results revealed that isolate UPMZ01 gave the optimum inhibitory percentage of (100%) at all concentrations of fungal secondary metabolites at 14 days. Based on ITS sequencing, the isolate UPMZ01 was identified as Aspergillus niger and UPMZ02 as Aspergillus flavus. The environmental factors such as pH and temperature influenced the production of secondary metabolites. The optimum condition for A. niger to produce efficient antifungal metabolites that gave (100%) PIGR against blast pathogen was at pH 5.0 and 21 to 29ºC. The secondary metabolites compounds were identified by gas chromatography-mass spectrometry (GC-MS). Fifteen compounds were recognized as major compounds. The identified compounds were Oleic Acid, n-Hexadecanoic acid, Hexose, Glycerol, Stearic acid, Tetradecanoic acid, Dodecanoic acid and 5-Hydrxoymethylfurfural. The Liquid chromatography-mass spectrophotometry (LC-MS/MS) analysis revealed four compounds, Succinic acid, Al21 anion, Diphenylarsine and N-Acetylaspartic acid. However, the culture filtrate of A. niger UPMZ01 may contribute to control rice blast pathogen. 2017-10 Thesis NonPeerReviewed text en http://psasir.upm.edu.my/id/eprint/70352/1/FP%202017%2025%20IR.pdf Idan, Ali Abdulameer (2017) Biological control of Pyricularia oryzae, using fungal metabolites. Masters thesis, Universiti Putra Malaysia.
institution Universiti Putra Malaysia
building UPM Library
collection Institutional Repository
continent Asia
country Malaysia
content_provider Universiti Putra Malaysia
content_source UPM Institutional Repository
url_provider http://psasir.upm.edu.my/
language English
description Rice blast caused by fungus Pyricularia oryzea is one of the most devastating among rice diseases. The disease has been classified as one of the most serious diseases that influence the production of rice since rice fields are seriously damaged, thereby affecting yield and quality. To decrease the use of pesticides, biological control is considered as an alternative sustainable control. Aspergillus spp. has been widely found to be useful in biotechnology as a bio control agent. They have a high ability in the production of secondary metabolites. Aspergillus spp. was obtained from the rice field soil located in Selangor, Tanjung Karang, Peninsular Malaysia. Isolates UPMZ01 and UPMZ02 were screened against blast pathogen by applying dual culture technique. The antagonism of isolate UPMZ01 was (81.326%) while UPMZ02 gave (70.9%) of inhibition percentage against P. oryzae. Culture filtrate was also used to screen the inhibitory effect against the pathogen. The results revealed that isolate UPMZ01 gave the optimum inhibitory percentage of (100%) at all concentrations of fungal secondary metabolites at 14 days. Based on ITS sequencing, the isolate UPMZ01 was identified as Aspergillus niger and UPMZ02 as Aspergillus flavus. The environmental factors such as pH and temperature influenced the production of secondary metabolites. The optimum condition for A. niger to produce efficient antifungal metabolites that gave (100%) PIGR against blast pathogen was at pH 5.0 and 21 to 29ºC. The secondary metabolites compounds were identified by gas chromatography-mass spectrometry (GC-MS). Fifteen compounds were recognized as major compounds. The identified compounds were Oleic Acid, n-Hexadecanoic acid, Hexose, Glycerol, Stearic acid, Tetradecanoic acid, Dodecanoic acid and 5-Hydrxoymethylfurfural. The Liquid chromatography-mass spectrophotometry (LC-MS/MS) analysis revealed four compounds, Succinic acid, Al21 anion, Diphenylarsine and N-Acetylaspartic acid. However, the culture filtrate of A. niger UPMZ01 may contribute to control rice blast pathogen.
format Thesis
author Idan, Ali Abdulameer
spellingShingle Idan, Ali Abdulameer
Biological control of Pyricularia oryzae, using fungal metabolites
author_facet Idan, Ali Abdulameer
author_sort Idan, Ali Abdulameer
title Biological control of Pyricularia oryzae, using fungal metabolites
title_short Biological control of Pyricularia oryzae, using fungal metabolites
title_full Biological control of Pyricularia oryzae, using fungal metabolites
title_fullStr Biological control of Pyricularia oryzae, using fungal metabolites
title_full_unstemmed Biological control of Pyricularia oryzae, using fungal metabolites
title_sort biological control of pyricularia oryzae, using fungal metabolites
publishDate 2017
url http://psasir.upm.edu.my/id/eprint/70352/1/FP%202017%2025%20IR.pdf
http://psasir.upm.edu.my/id/eprint/70352/
_version_ 1646008939508137984
score 13.211869

Similar Items