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Effect of pH and Temperature on The Binding of a Fusion Phage to Newcastle Disease Virus

A fusion phage carrying the peptide sequence CTLTTKLYC was selected from a disulfide constrained phage display random peptide library against Newcastle disease virus (NDV) strain AF2240. The binding affinity of the phage for AF2240 is both pH and temperature-dependent with optimal binding occurring...

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Main Authors: Wen, Siang Tan, Ramanujam, Priadarishni, Nathan, Sheila, Yusoff, Khatijah
Format: Article
Language:English
English
Published: 2004
Online Access:http://psasir.upm.edu.my/id/eprint/702/1/PFBSB4.pdf
http://psasir.upm.edu.my/id/eprint/702/
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spelling my.upm.eprints.7022013-05-27T06:50:15Z http://psasir.upm.edu.my/id/eprint/702/ Effect of pH and Temperature on The Binding of a Fusion Phage to Newcastle Disease Virus Wen, Siang Tan Ramanujam, Priadarishni Nathan, Sheila Yusoff, Khatijah A fusion phage carrying the peptide sequence CTLTTKLYC was selected from a disulfide constrained phage display random peptide library against Newcastle disease virus (NDV) strain AF2240. The binding affinity of the phage for AF2240 is both pH and temperature-dependent with optimal binding occurring at pH 4 and 4oC respectively. No binding was observed at pH 12. Binding decreased with temperatures above 4oC until it was completely abolished at 80oC. The results suggest that the fusion activity of the virus is promoted in acidic conditions. 2004 Article NonPeerReviewed application/pdf en http://psasir.upm.edu.my/id/eprint/702/1/PFBSB4.pdf Wen, Siang Tan and Ramanujam, Priadarishni and Nathan, Sheila and Yusoff, Khatijah (2004) Effect of pH and Temperature on The Binding of a Fusion Phage to Newcastle Disease Virus. Malaysian Journal of Biochemistry and Molecular Biology, 9. pp. 35-37. ISSN 1511-2616 English
institution Universiti Putra Malaysia
building UPM Library
collection Institutional Repository
continent Asia
country Malaysia
content_provider Universiti Putra Malaysia
content_source UPM Institutional Repository
url_provider http://psasir.upm.edu.my/
language English
English
description A fusion phage carrying the peptide sequence CTLTTKLYC was selected from a disulfide constrained phage display random peptide library against Newcastle disease virus (NDV) strain AF2240. The binding affinity of the phage for AF2240 is both pH and temperature-dependent with optimal binding occurring at pH 4 and 4oC respectively. No binding was observed at pH 12. Binding decreased with temperatures above 4oC until it was completely abolished at 80oC. The results suggest that the fusion activity of the virus is promoted in acidic conditions.
format Article
author Wen, Siang Tan
Ramanujam, Priadarishni
Nathan, Sheila
Yusoff, Khatijah
spellingShingle Wen, Siang Tan
Ramanujam, Priadarishni
Nathan, Sheila
Yusoff, Khatijah
Effect of pH and Temperature on The Binding of a Fusion Phage to Newcastle Disease Virus
author_facet Wen, Siang Tan
Ramanujam, Priadarishni
Nathan, Sheila
Yusoff, Khatijah
author_sort Wen, Siang Tan
title Effect of pH and Temperature on The Binding of a Fusion Phage to Newcastle Disease Virus
title_short Effect of pH and Temperature on The Binding of a Fusion Phage to Newcastle Disease Virus
title_full Effect of pH and Temperature on The Binding of a Fusion Phage to Newcastle Disease Virus
title_fullStr Effect of pH and Temperature on The Binding of a Fusion Phage to Newcastle Disease Virus
title_full_unstemmed Effect of pH and Temperature on The Binding of a Fusion Phage to Newcastle Disease Virus
title_sort effect of ph and temperature on the binding of a fusion phage to newcastle disease virus
publishDate 2004
url http://psasir.upm.edu.my/id/eprint/702/1/PFBSB4.pdf
http://psasir.upm.edu.my/id/eprint/702/
_version_ 1643821894823051264
score 13.244745

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