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Characterization and transfer of chloramphenicol resistance gene in bacteria isolated from aquaculture ponds

The presence of antibiotic residues and antibiotic resistant organisms in agriculture and aquaculture environments and products constitute a potential threat to the sustainable development and international trade in the industries. A total of 127 chloramphenicol resistance isolates obtained from sel...

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Main Author: Abdul Rahman, Noor Azrin
Format: Thesis
Language:English
Published: 2011
Subjects:
Chloramphenicol
Online Access:http://psasir.upm.edu.my/id/eprint/70102/1/FPV%202011%2021%20-%20IR.pdf
http://psasir.upm.edu.my/id/eprint/70102/
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spelling my.upm.eprints.701022019-11-12T02:31:27Z http://psasir.upm.edu.my/id/eprint/70102/ Characterization and transfer of chloramphenicol resistance gene in bacteria isolated from aquaculture ponds Abdul Rahman, Noor Azrin The presence of antibiotic residues and antibiotic resistant organisms in agriculture and aquaculture environments and products constitute a potential threat to the sustainable development and international trade in the industries. A total of 127 chloramphenicol resistance isolates obtained from selected aquaculture ponds in Selangor and Perak, Malaysia, were tested their susceptibility to common antibiotic used in aquaculture by using the disk diffusion method and minimal inhibitory concentration. Most of the chloramphenicol resistant isolates were also highly resistant to nitrofurantoin (80.3%), trimethoprim/ sulfamethoxazole (78%) and tetracycline (74.8%). The minimal inhibitory concentration (MIC) analysis showed that most of the isolates were resistant to chloramphenicol at MIC value > 1024 ppm. The chloramphenicol resistance genes detection in the isolates using multiplex PCR assay shown that, two chloramphenicol resistant genes (cat I and cat II) were successfully amplified from 51 out of 127 isolates. The assay determined that cat II was the dominant gene occurring in 47 out of 127 isolates. Forty one of 76 isolates that did not carry positive cat genes, gave positive changes in biochemical cat assay. Of the 51 cat gene positive isolates, 35 (69%) isolates harbored plasmids. Among the 35 isolates, 21 were observed to harbor plasmid and susceptible to streptomycin (> 6mm to STR10μg disk) and possibility of chloramphenicol resistance gene transfer from these positive isolates to naive bacteria via conjugation were tested by using plate mating method. However, the transfer of chloramphenicol resistant gene was not clearly detected in any isolate. 2011-08 Thesis NonPeerReviewed text en http://psasir.upm.edu.my/id/eprint/70102/1/FPV%202011%2021%20-%20IR.pdf Abdul Rahman, Noor Azrin (2011) Characterization and transfer of chloramphenicol resistance gene in bacteria isolated from aquaculture ponds. Masters thesis, Universiti Putra Malaysia. Chloramphenicol
institution Universiti Putra Malaysia
building UPM Library
collection Institutional Repository
continent Asia
country Malaysia
content_provider Universiti Putra Malaysia
content_source UPM Institutional Repository
url_provider http://psasir.upm.edu.my/
language English
topic Chloramphenicol
spellingShingle Chloramphenicol
Abdul Rahman, Noor Azrin
Characterization and transfer of chloramphenicol resistance gene in bacteria isolated from aquaculture ponds
description The presence of antibiotic residues and antibiotic resistant organisms in agriculture and aquaculture environments and products constitute a potential threat to the sustainable development and international trade in the industries. A total of 127 chloramphenicol resistance isolates obtained from selected aquaculture ponds in Selangor and Perak, Malaysia, were tested their susceptibility to common antibiotic used in aquaculture by using the disk diffusion method and minimal inhibitory concentration. Most of the chloramphenicol resistant isolates were also highly resistant to nitrofurantoin (80.3%), trimethoprim/ sulfamethoxazole (78%) and tetracycline (74.8%). The minimal inhibitory concentration (MIC) analysis showed that most of the isolates were resistant to chloramphenicol at MIC value > 1024 ppm. The chloramphenicol resistance genes detection in the isolates using multiplex PCR assay shown that, two chloramphenicol resistant genes (cat I and cat II) were successfully amplified from 51 out of 127 isolates. The assay determined that cat II was the dominant gene occurring in 47 out of 127 isolates. Forty one of 76 isolates that did not carry positive cat genes, gave positive changes in biochemical cat assay. Of the 51 cat gene positive isolates, 35 (69%) isolates harbored plasmids. Among the 35 isolates, 21 were observed to harbor plasmid and susceptible to streptomycin (> 6mm to STR10μg disk) and possibility of chloramphenicol resistance gene transfer from these positive isolates to naive bacteria via conjugation were tested by using plate mating method. However, the transfer of chloramphenicol resistant gene was not clearly detected in any isolate.
format Thesis
author Abdul Rahman, Noor Azrin
author_facet Abdul Rahman, Noor Azrin
author_sort Abdul Rahman, Noor Azrin
title Characterization and transfer of chloramphenicol resistance gene in bacteria isolated from aquaculture ponds
title_short Characterization and transfer of chloramphenicol resistance gene in bacteria isolated from aquaculture ponds
title_full Characterization and transfer of chloramphenicol resistance gene in bacteria isolated from aquaculture ponds
title_fullStr Characterization and transfer of chloramphenicol resistance gene in bacteria isolated from aquaculture ponds
title_full_unstemmed Characterization and transfer of chloramphenicol resistance gene in bacteria isolated from aquaculture ponds
title_sort characterization and transfer of chloramphenicol resistance gene in bacteria isolated from aquaculture ponds
publishDate 2011
url http://psasir.upm.edu.my/id/eprint/70102/1/FPV%202011%2021%20-%20IR.pdf
http://psasir.upm.edu.my/id/eprint/70102/
_version_ 1651869115066875904
score 13.211869

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