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Characterization, pathogenicity and host range analyses of Lasiodiplodia sp. and related species isolated from fruit rot disease of mango in Peninsular Malaysia

Lasiodiplodia species is an important plant pathogen causing fruit rot, a threatening disease for mango fruits in Malaysia. Capability of thespecies to infect during preand post-harvest phases will reduced mango production and creating tremendous losses in country economic. The information reg...

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Main Author: Mohd Sattar, Munirah
Format: Thesis
Language:English
Published: 2017
Subjects:
Fungal diseases of plants - Research - Malaysia
Botryosphaeriaceae
Mango - Diseases and pests
Online Access:http://psasir.upm.edu.my/id/eprint/69780/1/fs%202017%2060%20ir.pdf
http://psasir.upm.edu.my/id/eprint/69780/
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spelling my.upm.eprints.697802019-11-12T00:32:42Z http://psasir.upm.edu.my/id/eprint/69780/ Characterization, pathogenicity and host range analyses of Lasiodiplodia sp. and related species isolated from fruit rot disease of mango in Peninsular Malaysia Mohd Sattar, Munirah Lasiodiplodia species is an important plant pathogen causing fruit rot, a threatening disease for mango fruits in Malaysia. Capability of thespecies to infect during preand post-harvest phases will reduced mango production and creating tremendous losses in country economic. The information regarding pre-harvest fruit rot disease on mango in Malaysia is still lacking, therefore, this study was conducted. During a series of sampling in July 2014 to May 2015 throughout Peninsular Malaysia, the symptom of fruit rot was observed in the Malaysian mango recommended varieties such as Chok Anan (MA224), Harumanis (MA128), Sala and Epel. Thus, the objectives of this study were to isolate and identify fungi species isolated from mango fruit rot disease based on morphological and molecular approaches using internal transcribed spacer (ITS) and β-tubulin gene (BT2) sequence, to determine whether or not Lasiodiplodia, Pseudofusicoccum and Neofusicoccum isolates are pathogenic and to examine the host range of Lasiodiplodia theobromae, Lasiodiplodia pseudotheobromae and Pseudofusicoccum adansoniae as causal pathogens of mango fruit rot disease in selected commercial fruits. There are 26 isolates were purified and successfully identified as Lasiodiplodia theobromae (17 isolates), Lasiodiplodia pseudotheobromae (3 isolates), Pseudofusicoccum adansoniae (5 isolates) and Neofusicoccum parvum (1 isolate). A combined phylogenetic tree was constructed using maximum-likelihood method showed the same species grouped into the same clade. Clade I consisted of L. theobromae, L. pseudotheobromae and N. parvum while Clade II were P. adansoniae species. For the pathogenicity test, all isolates of Lasiodiplodia species inoculated on healthy mango fruit using non-wounded method are pathogenic and showed fruit rot symptom except for L. pseudotheobromae (B1494). Whereas, for P. adansoniae and N. parvum species isolates, only one isolate of P. adansoniae (B1474) produced fruit rot symptom. The most virulent isolate was L. theobromae (A1718) with disease severity index, DSI of 87.50%. To examine virulence of the pathogenic isolates obtained from mango samples on different hosts, each species isolates with highest DSI in pathogenicity test were inoculated on seven commercial fruits such as apple, banana, musk lime, guava, mandarin, pear and sapodilla. The host range study confirmed that L. theobromae, L. pseudotheobromae and P. adansoniae were pathogenic to apple, musk lime, guava, mandarin and sapodilla with varies in disease severity. As conclusion, L. theobromae, L. pseudotheobromae and P. adansoniae were identified and confirmed as pathogens of pre-harvest fruit rots disease on mango in Malaysia. Those three species are also potential pathogen for apple, banana, musk lime, guava, mandarin and sapodilla if dissemination of inoculums were occurred during pre- or post-harvest stages.This study represents the first report of L. pseudotheobromae and P. adansoniae on pre-harvest mango in Peninsular Malaysia associated with fruit rot disease. 2017-04 Thesis NonPeerReviewed text en http://psasir.upm.edu.my/id/eprint/69780/1/fs%202017%2060%20ir.pdf Mohd Sattar, Munirah (2017) Characterization, pathogenicity and host range analyses of Lasiodiplodia sp. and related species isolated from fruit rot disease of mango in Peninsular Malaysia. Masters thesis, Universiti Putra Malaysia. Fungal diseases of plants - Research - Malaysia Botryosphaeriaceae Mango - Diseases and pests
institution Universiti Putra Malaysia
building UPM Library
collection Institutional Repository
continent Asia
country Malaysia
content_provider Universiti Putra Malaysia
content_source UPM Institutional Repository
url_provider http://psasir.upm.edu.my/
language English
topic Fungal diseases of plants - Research - Malaysia
Botryosphaeriaceae
Mango - Diseases and pests
spellingShingle Fungal diseases of plants - Research - Malaysia
Botryosphaeriaceae
Mango - Diseases and pests
Mohd Sattar, Munirah
Characterization, pathogenicity and host range analyses of Lasiodiplodia sp. and related species isolated from fruit rot disease of mango in Peninsular Malaysia
description Lasiodiplodia species is an important plant pathogen causing fruit rot, a threatening disease for mango fruits in Malaysia. Capability of thespecies to infect during preand post-harvest phases will reduced mango production and creating tremendous losses in country economic. The information regarding pre-harvest fruit rot disease on mango in Malaysia is still lacking, therefore, this study was conducted. During a series of sampling in July 2014 to May 2015 throughout Peninsular Malaysia, the symptom of fruit rot was observed in the Malaysian mango recommended varieties such as Chok Anan (MA224), Harumanis (MA128), Sala and Epel. Thus, the objectives of this study were to isolate and identify fungi species isolated from mango fruit rot disease based on morphological and molecular approaches using internal transcribed spacer (ITS) and β-tubulin gene (BT2) sequence, to determine whether or not Lasiodiplodia, Pseudofusicoccum and Neofusicoccum isolates are pathogenic and to examine the host range of Lasiodiplodia theobromae, Lasiodiplodia pseudotheobromae and Pseudofusicoccum adansoniae as causal pathogens of mango fruit rot disease in selected commercial fruits. There are 26 isolates were purified and successfully identified as Lasiodiplodia theobromae (17 isolates), Lasiodiplodia pseudotheobromae (3 isolates), Pseudofusicoccum adansoniae (5 isolates) and Neofusicoccum parvum (1 isolate). A combined phylogenetic tree was constructed using maximum-likelihood method showed the same species grouped into the same clade. Clade I consisted of L. theobromae, L. pseudotheobromae and N. parvum while Clade II were P. adansoniae species. For the pathogenicity test, all isolates of Lasiodiplodia species inoculated on healthy mango fruit using non-wounded method are pathogenic and showed fruit rot symptom except for L. pseudotheobromae (B1494). Whereas, for P. adansoniae and N. parvum species isolates, only one isolate of P. adansoniae (B1474) produced fruit rot symptom. The most virulent isolate was L. theobromae (A1718) with disease severity index, DSI of 87.50%. To examine virulence of the pathogenic isolates obtained from mango samples on different hosts, each species isolates with highest DSI in pathogenicity test were inoculated on seven commercial fruits such as apple, banana, musk lime, guava, mandarin, pear and sapodilla. The host range study confirmed that L. theobromae, L. pseudotheobromae and P. adansoniae were pathogenic to apple, musk lime, guava, mandarin and sapodilla with varies in disease severity. As conclusion, L. theobromae, L. pseudotheobromae and P. adansoniae were identified and confirmed as pathogens of pre-harvest fruit rots disease on mango in Malaysia. Those three species are also potential pathogen for apple, banana, musk lime, guava, mandarin and sapodilla if dissemination of inoculums were occurred during pre- or post-harvest stages.This study represents the first report of L. pseudotheobromae and P. adansoniae on pre-harvest mango in Peninsular Malaysia associated with fruit rot disease.
format Thesis
author Mohd Sattar, Munirah
author_facet Mohd Sattar, Munirah
author_sort Mohd Sattar, Munirah
title Characterization, pathogenicity and host range analyses of Lasiodiplodia sp. and related species isolated from fruit rot disease of mango in Peninsular Malaysia
title_short Characterization, pathogenicity and host range analyses of Lasiodiplodia sp. and related species isolated from fruit rot disease of mango in Peninsular Malaysia
title_full Characterization, pathogenicity and host range analyses of Lasiodiplodia sp. and related species isolated from fruit rot disease of mango in Peninsular Malaysia
title_fullStr Characterization, pathogenicity and host range analyses of Lasiodiplodia sp. and related species isolated from fruit rot disease of mango in Peninsular Malaysia
title_full_unstemmed Characterization, pathogenicity and host range analyses of Lasiodiplodia sp. and related species isolated from fruit rot disease of mango in Peninsular Malaysia
title_sort characterization, pathogenicity and host range analyses of lasiodiplodia sp. and related species isolated from fruit rot disease of mango in peninsular malaysia
publishDate 2017
url http://psasir.upm.edu.my/id/eprint/69780/1/fs%202017%2060%20ir.pdf
http://psasir.upm.edu.my/id/eprint/69780/
_version_ 1651869099194580992
score 13.211869

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