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Physicochemical characterization and In vitro anti-cancer effects of iron-manganese and iron-doped sulfated zirconia nanoparticles in cancer cell lines

Crystal nanoparticle is a new system with potential as a therapeutic agent in the treatment of diseases. The objectives of this study are to synthesize, characterize and determine the anticancer cell effects of iron-manganese and iron-doped sulfated zirconia nanoparticles. In this study the iron-...

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Main Author: Khlaif, Mohamed Qasim
Format: Thesis
Language:English
Published: 2016
Online Access:http://psasir.upm.edu.my/id/eprint/69706/1/IB%202016%204%20IR.pdf
http://psasir.upm.edu.my/id/eprint/69706/
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spelling my.upm.eprints.697062019-09-20T02:59:23Z http://psasir.upm.edu.my/id/eprint/69706/ Physicochemical characterization and In vitro anti-cancer effects of iron-manganese and iron-doped sulfated zirconia nanoparticles in cancer cell lines Khlaif, Mohamed Qasim Crystal nanoparticle is a new system with potential as a therapeutic agent in the treatment of diseases. The objectives of this study are to synthesize, characterize and determine the anticancer cell effects of iron-manganese and iron-doped sulfated zirconia nanoparticles. In this study the iron-manganese- and iron-doped sulfated zirconia nanoparticles were prepared by hydrothermal impregnation method followed by calcination. The characterization of both nanoparticles were carried out using X-ray diffraction (XRD), thermal gravimetric analysis (TGA), fourier transform infrared spectroscopy (FT-IR), Brunner-Emmett-Teller (BET) surface area measurements, Xray fluorescence (XRF), X-ray photoelectron spectroscopy, zeta potential (ZSP) measurement, and transmission electron microscopy (TEM). The cytotoxicity of the nanoparticles was determined via the 3-(4,5-dimethylthiazol-2-yl)-2,5- diphenyltetrazolium bromide assay on human breast adenocarcinoma (MDA-MB-231), colorectal adenocarcinoma (HT29), and hepatocellular carcinoma (HepG2) cell lines, and two normal human cell lines, the Chang liver and human umbilical vein endothelial cells (HUVEC) cell lines. The results showed that the iron-manganese- and iron-doped sulfated zirconia nanoparticles were of average size 12.7 and 32.0 nm, respectively and zeta potential of 15.0 and 0.206 mV, respectively. These nanoparticles tend to aggregate in solution. The iron-manganese- and iron-doped sulfated zirconia nanoparticles are highly toxic to the MDA-MB-231 and HepG2 cells, respectively, showing dramatic morphological changes suggesting loss of cell viability. The nanoparticles are comparatively less toxic to the HT29 cells compared to the other cancer cell lines. The study suggests that the anticancer effects of iron-manganese- and iron-doped sulfated zirconia nanoparticles implicate caspase-3, 8 and -9 in their anticancer cells activities. The findings from the study highlight the potential of ironmanganese- and iron-doped sulfated zirconia nanoparticles as therapeutic agents in the treatment of cancers, while showing lesser effect on normal cells. 2016-04 Thesis NonPeerReviewed text en http://psasir.upm.edu.my/id/eprint/69706/1/IB%202016%204%20IR.pdf Khlaif, Mohamed Qasim (2016) Physicochemical characterization and In vitro anti-cancer effects of iron-manganese and iron-doped sulfated zirconia nanoparticles in cancer cell lines. Masters thesis, Universiti Putra Malaysia.
institution Universiti Putra Malaysia
building UPM Library
collection Institutional Repository
continent Asia
country Malaysia
content_provider Universiti Putra Malaysia
content_source UPM Institutional Repository
url_provider http://psasir.upm.edu.my/
language English
description Crystal nanoparticle is a new system with potential as a therapeutic agent in the treatment of diseases. The objectives of this study are to synthesize, characterize and determine the anticancer cell effects of iron-manganese and iron-doped sulfated zirconia nanoparticles. In this study the iron-manganese- and iron-doped sulfated zirconia nanoparticles were prepared by hydrothermal impregnation method followed by calcination. The characterization of both nanoparticles were carried out using X-ray diffraction (XRD), thermal gravimetric analysis (TGA), fourier transform infrared spectroscopy (FT-IR), Brunner-Emmett-Teller (BET) surface area measurements, Xray fluorescence (XRF), X-ray photoelectron spectroscopy, zeta potential (ZSP) measurement, and transmission electron microscopy (TEM). The cytotoxicity of the nanoparticles was determined via the 3-(4,5-dimethylthiazol-2-yl)-2,5- diphenyltetrazolium bromide assay on human breast adenocarcinoma (MDA-MB-231), colorectal adenocarcinoma (HT29), and hepatocellular carcinoma (HepG2) cell lines, and two normal human cell lines, the Chang liver and human umbilical vein endothelial cells (HUVEC) cell lines. The results showed that the iron-manganese- and iron-doped sulfated zirconia nanoparticles were of average size 12.7 and 32.0 nm, respectively and zeta potential of 15.0 and 0.206 mV, respectively. These nanoparticles tend to aggregate in solution. The iron-manganese- and iron-doped sulfated zirconia nanoparticles are highly toxic to the MDA-MB-231 and HepG2 cells, respectively, showing dramatic morphological changes suggesting loss of cell viability. The nanoparticles are comparatively less toxic to the HT29 cells compared to the other cancer cell lines. The study suggests that the anticancer effects of iron-manganese- and iron-doped sulfated zirconia nanoparticles implicate caspase-3, 8 and -9 in their anticancer cells activities. The findings from the study highlight the potential of ironmanganese- and iron-doped sulfated zirconia nanoparticles as therapeutic agents in the treatment of cancers, while showing lesser effect on normal cells.
format Thesis
author Khlaif, Mohamed Qasim
spellingShingle Khlaif, Mohamed Qasim
Physicochemical characterization and In vitro anti-cancer effects of iron-manganese and iron-doped sulfated zirconia nanoparticles in cancer cell lines
author_facet Khlaif, Mohamed Qasim
author_sort Khlaif, Mohamed Qasim
title Physicochemical characterization and In vitro anti-cancer effects of iron-manganese and iron-doped sulfated zirconia nanoparticles in cancer cell lines
title_short Physicochemical characterization and In vitro anti-cancer effects of iron-manganese and iron-doped sulfated zirconia nanoparticles in cancer cell lines
title_full Physicochemical characterization and In vitro anti-cancer effects of iron-manganese and iron-doped sulfated zirconia nanoparticles in cancer cell lines
title_fullStr Physicochemical characterization and In vitro anti-cancer effects of iron-manganese and iron-doped sulfated zirconia nanoparticles in cancer cell lines
title_full_unstemmed Physicochemical characterization and In vitro anti-cancer effects of iron-manganese and iron-doped sulfated zirconia nanoparticles in cancer cell lines
title_sort physicochemical characterization and in vitro anti-cancer effects of iron-manganese and iron-doped sulfated zirconia nanoparticles in cancer cell lines
publishDate 2016
url http://psasir.upm.edu.my/id/eprint/69706/1/IB%202016%204%20IR.pdf
http://psasir.upm.edu.my/id/eprint/69706/
_version_ 1646008933801787392
score 13.211869

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