Physicochemical characterization and In vitro anti-cancer effects of iron-manganese and iron-doped sulfated zirconia nanoparticles in cancer cell lines
Crystal nanoparticle is a new system with potential as a therapeutic agent in the treatment of diseases. The objectives of this study are to synthesize, characterize and determine the anticancer cell effects of iron-manganese and iron-doped sulfated zirconia nanoparticles. In this study the iron-...
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| フォーマット: | 学位論文 |
| 言語: | English |
| 出版事項: |
2016
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| オンライン･アクセス: | http://psasir.upm.edu.my/id/eprint/69706/1/IB%202016%204%20IR.pdf http://psasir.upm.edu.my/id/eprint/69706/ |
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| 要約: | Crystal nanoparticle is a new system with potential as a therapeutic agent in the
treatment of diseases. The objectives of this study are to synthesize, characterize and
determine the anticancer cell effects of iron-manganese and iron-doped sulfated
zirconia nanoparticles. In this study the iron-manganese- and iron-doped sulfated
zirconia nanoparticles were prepared by hydrothermal impregnation method followed
by calcination. The characterization of both nanoparticles were carried out using X-ray
diffraction (XRD), thermal gravimetric analysis (TGA), fourier transform infrared
spectroscopy (FT-IR), Brunner-Emmett-Teller (BET) surface area measurements, Xray
fluorescence (XRF), X-ray photoelectron spectroscopy, zeta potential (ZSP)
measurement, and transmission electron microscopy (TEM). The cytotoxicity of the
nanoparticles was determined via the 3-(4,5-dimethylthiazol-2-yl)-2,5-
diphenyltetrazolium bromide assay on human breast adenocarcinoma (MDA-MB-231),
colorectal adenocarcinoma (HT29), and hepatocellular carcinoma (HepG2) cell lines,
and two normal human cell lines, the Chang liver and human umbilical vein endothelial
cells (HUVEC) cell lines. The results showed that the iron-manganese- and iron-doped
sulfated zirconia nanoparticles were of average size 12.7 and 32.0 nm, respectively and
zeta potential of 15.0 and 0.206 mV, respectively. These nanoparticles tend to
aggregate in solution. The iron-manganese- and iron-doped sulfated zirconia
nanoparticles are highly toxic to the MDA-MB-231 and HepG2 cells, respectively,
showing dramatic morphological changes suggesting loss of cell viability. The
nanoparticles are comparatively less toxic to the HT29 cells compared to the other
cancer cell lines. The study suggests that the anticancer effects of iron-manganese- and
iron-doped sulfated zirconia nanoparticles implicate caspase-3, 8 and -9 in their
anticancer cells activities. The findings from the study highlight the potential of ironmanganese-
and iron-doped sulfated zirconia nanoparticles as therapeutic agents in the
treatment of cancers, while showing lesser effect on normal cells. |
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