Assessment of selected microalgae (Spirulina sp., Nannochloropsis sp. and Chlorella sp.) as feed supplement for Macrobrachium rosenbergii (de Man) juveniles
A series of experiment were conducted to study the potential of feed formulation for freshwater prawn Macrobrachium rosenbergii juvenile. Study on the potential of selected microalgae (Spirulina sp., Nannochloropsis sp. and Chlorella sp.) as feed for M. rosenbergii juvenile was conducted with...
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| Format: | Thesis |
| Language: | English |
| Published: |
2017
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| Online Access: | http://psasir.upm.edu.my/id/eprint/69629/1/fp%202017%2056%20ir.pdf http://psasir.upm.edu.my/id/eprint/69629/ |
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| Summary: | A series of experiment were conducted to study the potential of feed formulation for
freshwater prawn Macrobrachium rosenbergii juvenile. Study on the potential of
selected microalgae (Spirulina sp., Nannochloropsis sp. and Chlorella sp.) as feed for
M. rosenbergii juvenile was conducted with amino acid and fatty acid profile. From
the results, methionine and tryptophan in amino acid were not detected in Spirulina
sp., Nannochloropsis sp. and Chlorella sp. in all four different levels. The arginine,
isoleucine, leucine, alanine, aspartic acid and glutamic acid level were high with
increasing level of Spirulina sp. Nannochloropsis sp. and Chlorella sp. showed high
percentage in leucine, lysine and arginine. For fatty acid analysis, only Spirulina
showed a small portion of γ–linolenic acid (GLA). Chlorella showed no detection on
Eicosapentaenoic acid (EPA) when compared to Spirulina and Nannochloropsis.
For second experiment, a series of experiments were conducted to study the potential
of the feed formulations for freshwater prawn Macrobrachium rosenbergii juvenile
based on three types of microalgae (Spirulina sp., Nanochloropsis sp. and Chlorella
sp.). An experiment in triplicate with prawn (2.5-2.6 g) were fed until an apparent
satiation with 32% crude protein diet containing three types of algae with different
level (Diet 1= 0 %, Diet 2=2.5 %, Diet 3= 5 % and Diet 4= 10%) in varying algae
levels ranging from 0, 2.5, 5 and 10% respectively for 56 days. At the end of the
feeding trial, prawn given Diet 4 containing 10 % of Spirulina sp. gave the optimum
growth which also contributed to the best feed efficiency. There was no significant
difference in FCR in all treatments (p<0.05). There were significant differences
(p<0.05) in growth rate among treatments and similarly prawns fed Diet 4 in Spirulina
sp. had the highest weight gain (WG) and specific growth rate (SGR) 5.53 g and 2.05
%/day, respectively. The percentage survival of M. rosenbergii ranged from 63-83 %.
This study also demonstrated that the nutrient from Spirulina sp. is more suitable to be supplemented in prawn diet compared with Nannochloropsis sp. and Chlorella sp. The
used of Cr2O3 marker is reliable for digestibility study.
Spirulina sp., Nannochloropsis sp. and Chlorella sp. were analyzed for antimicrobial
activity to determine the potential of the algae against the selected pathogens.
Chlorella sp. had successfully inhibited Aeromonas hydrophilla followed by A.
salmonicida and Vibrio alginolyticus. The maximum inhibition zone by Chlorella sp.
were 14.67 mm against A. hydrophila, followed by 11.33 mm against V. alginolyticus
and 10.00 mm against A. sobria. Studies on the effect of Spirulina sp, Nannochloropsis
sp. and Chlorella sp. on prawn challenged with A. hydrophilla for 48 hours showed
there are no significant difference among the different treatments. Prawn’s mortality
was 100 % in control group.
Spirulina sp. with 10% algae level showed a good potential to be as a feed supplement
for M. rosenbergii juveniles. Spirulina sp. contained a variety of amino acids and fatty
acids to support good growth performance, digestibility and disease resistance as
compared to Nannochloropsis sp. and Chlorella sp. |
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