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Antioxidant properties and toxicity assessment of the Crescentia cujete extracts in brine shrimp (Artemia salina)

Crescentia cujete has traditionally been used to treat various ailments. The present study attempted to determine the antioxidant activities of 100% ethanol, 50% ethanol and aqueous extracts of the leaves, bark and fruit of the herb. Additionally, the toxicity of the extracts was investigated in bri...

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Main Authors: Sandra Sagrin, Mozhiarhasi, Lasano, Nur Fatimah, Shukri, Radhiah, Ramli, Nurul Shazini
Format: Article
Language:English
Published: Penerbit Universiti Kebangsaan Malaysia 2019
Online Access:http://psasir.upm.edu.my/id/eprint/69307/1/Antioxidant%20properties%20and%20toxicity%20assessment%20of%20the%20Crescentia%20cujete%20extracts%20in%20brine%20shrimp%20%28Artemia%20salina%29.pdf
http://psasir.upm.edu.my/id/eprint/69307/
http://www.ukm.my/jsm/english_journals/vol48num4_2019/contentsVol48num4_2019.html
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spelling my.upm.eprints.693072020-03-25T09:15:15Z http://psasir.upm.edu.my/id/eprint/69307/ Antioxidant properties and toxicity assessment of the Crescentia cujete extracts in brine shrimp (Artemia salina) Sandra Sagrin, Mozhiarhasi Lasano, Nur Fatimah Shukri, Radhiah Ramli, Nurul Shazini Crescentia cujete has traditionally been used to treat various ailments. The present study attempted to determine the antioxidant activities of 100% ethanol, 50% ethanol and aqueous extracts of the leaves, bark and fruit of the herb. Additionally, the toxicity of the extracts was investigated in brine shrimp. The results showed that 100% ethanol leaf extract had the highest antioxidant activity with an IC50 value of 261.97 ± 0.57 μg/mL according to the 2,2-diphenyl-1-picrylhydrazil (DPPH) radical scavenging assay. Estimation of the total phenolic content (TPC) using the Folin-Ciocalteu reagent showed that the leaves extracted with 100% ethanol had the highest concentration of TPC compared to the extracts prepared with other solvents of all parts of the plant. Analysis of the minerals using inductively coupled plasma atomic emission spectroscopy (ICP-OES) showed that the parts of the plant primarily consist of high levels of phosphorus, magnesium and calcium. Titration analysis of vitamin C showed that the highest concentration of the vitamin is present in the bark. High performance liquid chromatography (HPLC) analysis of vitamin E indicated that the content is the highest in the fruit, and the content of vitamin A was the highest in the leaves. Brine shrimp lethality assay (BSLA) results showed that 50% ethanol extract of the leaves is the most toxic during a 24 h treatment. Thus, the leaves and bark exhibit excellent antioxidant effects and can be potentially developed as functional food ingredients. The findings of the present study suggest further research in cell lines and in vivo. Penerbit Universiti Kebangsaan Malaysia 2019 Article PeerReviewed text en http://psasir.upm.edu.my/id/eprint/69307/1/Antioxidant%20properties%20and%20toxicity%20assessment%20of%20the%20Crescentia%20cujete%20extracts%20in%20brine%20shrimp%20%28Artemia%20salina%29.pdf Sandra Sagrin, Mozhiarhasi and Lasano, Nur Fatimah and Shukri, Radhiah and Ramli, Nurul Shazini (2019) Antioxidant properties and toxicity assessment of the Crescentia cujete extracts in brine shrimp (Artemia salina). Sains Malaysiana, 48 (4). pp. 831-840. ISSN 0126-6039 http://www.ukm.my/jsm/english_journals/vol48num4_2019/contentsVol48num4_2019.html 10.17576/jsm-2019-4804-15
institution Universiti Putra Malaysia
building UPM Library
collection Institutional Repository
continent Asia
country Malaysia
content_provider Universiti Putra Malaysia
content_source UPM Institutional Repository
url_provider http://psasir.upm.edu.my/
language English
description Crescentia cujete has traditionally been used to treat various ailments. The present study attempted to determine the antioxidant activities of 100% ethanol, 50% ethanol and aqueous extracts of the leaves, bark and fruit of the herb. Additionally, the toxicity of the extracts was investigated in brine shrimp. The results showed that 100% ethanol leaf extract had the highest antioxidant activity with an IC50 value of 261.97 ± 0.57 μg/mL according to the 2,2-diphenyl-1-picrylhydrazil (DPPH) radical scavenging assay. Estimation of the total phenolic content (TPC) using the Folin-Ciocalteu reagent showed that the leaves extracted with 100% ethanol had the highest concentration of TPC compared to the extracts prepared with other solvents of all parts of the plant. Analysis of the minerals using inductively coupled plasma atomic emission spectroscopy (ICP-OES) showed that the parts of the plant primarily consist of high levels of phosphorus, magnesium and calcium. Titration analysis of vitamin C showed that the highest concentration of the vitamin is present in the bark. High performance liquid chromatography (HPLC) analysis of vitamin E indicated that the content is the highest in the fruit, and the content of vitamin A was the highest in the leaves. Brine shrimp lethality assay (BSLA) results showed that 50% ethanol extract of the leaves is the most toxic during a 24 h treatment. Thus, the leaves and bark exhibit excellent antioxidant effects and can be potentially developed as functional food ingredients. The findings of the present study suggest further research in cell lines and in vivo.
format Article
author Sandra Sagrin, Mozhiarhasi
Lasano, Nur Fatimah
Shukri, Radhiah
Ramli, Nurul Shazini
spellingShingle Sandra Sagrin, Mozhiarhasi
Lasano, Nur Fatimah
Shukri, Radhiah
Ramli, Nurul Shazini
Antioxidant properties and toxicity assessment of the Crescentia cujete extracts in brine shrimp (Artemia salina)
author_facet Sandra Sagrin, Mozhiarhasi
Lasano, Nur Fatimah
Shukri, Radhiah
Ramli, Nurul Shazini
author_sort Sandra Sagrin, Mozhiarhasi
title Antioxidant properties and toxicity assessment of the Crescentia cujete extracts in brine shrimp (Artemia salina)
title_short Antioxidant properties and toxicity assessment of the Crescentia cujete extracts in brine shrimp (Artemia salina)
title_full Antioxidant properties and toxicity assessment of the Crescentia cujete extracts in brine shrimp (Artemia salina)
title_fullStr Antioxidant properties and toxicity assessment of the Crescentia cujete extracts in brine shrimp (Artemia salina)
title_full_unstemmed Antioxidant properties and toxicity assessment of the Crescentia cujete extracts in brine shrimp (Artemia salina)
title_sort antioxidant properties and toxicity assessment of the crescentia cujete extracts in brine shrimp (artemia salina)
publisher Penerbit Universiti Kebangsaan Malaysia
publishDate 2019
url http://psasir.upm.edu.my/id/eprint/69307/1/Antioxidant%20properties%20and%20toxicity%20assessment%20of%20the%20Crescentia%20cujete%20extracts%20in%20brine%20shrimp%20%28Artemia%20salina%29.pdf
http://psasir.upm.edu.my/id/eprint/69307/
http://www.ukm.my/jsm/english_journals/vol48num4_2019/contentsVol48num4_2019.html
_version_ 1665895988772995072
score 13.211869

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