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Effects of N-methyl-N-nitrosourea on seed germination of Stevia rebaudiana

Stevia rebaudiana is a unique plant that contains non-caloric natural sweetener and has gained much interest among Malaysians. In this study, the effect of different concentrations of N‑methyl-N-nitrosourea (MNU) was assessed in inducing mutation in Stevia seeds to produce genetic variations, which...

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Main Authors: Ahmad, Lyena Watty Zuraine, Zainuddin, Zarina, Osman, Mohamad, Mohamad, Azhar, Jaafar Sidik, Norrizah
Format: Article
Language:English
Published: Penerbit Universiti Kebangsaan Malaysia 2019
Online Access:http://psasir.upm.edu.my/id/eprint/69286/1/Effects%20of%20N-methyl-N-nitrosourea%20on%20seed%20germination%20of%20Stevia%20rebaudiana.pdf
http://psasir.upm.edu.my/id/eprint/69286/
http://www.ukm.my/jsm/english_journals/vol48num2_2019/contentsVol48num2_2019.html
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spelling my.upm.eprints.692862020-03-25T09:03:06Z http://psasir.upm.edu.my/id/eprint/69286/ Effects of N-methyl-N-nitrosourea on seed germination of Stevia rebaudiana Ahmad, Lyena Watty Zuraine Zainuddin, Zarina Osman, Mohamad Mohamad, Azhar Jaafar Sidik, Norrizah Stevia rebaudiana is a unique plant that contains non-caloric natural sweetener and has gained much interest among Malaysians. In this study, the effect of different concentrations of N‑methyl-N-nitrosourea (MNU) was assessed in inducing mutation in Stevia seeds to produce genetic variations, which is valuable for crop improvement. Stevia seeds were soaked in six concentrations of MNU (0.0, 0.13, 0.25, 0.38, 0.50, and 1.00 mM) for four different durations (15, 30, 45, and 60 min) at room temperature. As a result, application of MNU reduced the germination percentage and germination rate of Stevia seeds as compared to the control group. Prolonged exposure to the highest concentration of MNU recorded the lowest percentage of germination (2.5 ± 1.4%) and the lowest germination rate (0.21 ± 0.16). Tricots were observed among seedlings treated with 0.13, 0.38 and 1.0 mM of MNU for 30 min. Presence of seedlings with albino colour proved the mutagenic effect of MNU on Stevia genome. Based on the percentage of seedlings with chlorophyll mutation, the most effective and efficient mutagenic treatment to induce mutation was 60 min in 0.25 mM of MNU. Penerbit Universiti Kebangsaan Malaysia 2019 Article PeerReviewed text en http://psasir.upm.edu.my/id/eprint/69286/1/Effects%20of%20N-methyl-N-nitrosourea%20on%20seed%20germination%20of%20Stevia%20rebaudiana.pdf Ahmad, Lyena Watty Zuraine and Zainuddin, Zarina and Osman, Mohamad and Mohamad, Azhar and Jaafar Sidik, Norrizah (2019) Effects of N-methyl-N-nitrosourea on seed germination of Stevia rebaudiana. Sains Malaysiana, 48 (2). pp. 301-307. ISSN 0126-6039 http://www.ukm.my/jsm/english_journals/vol48num2_2019/contentsVol48num2_2019.html 10.17576/jsm-2019-4802-05
institution Universiti Putra Malaysia
building UPM Library
collection Institutional Repository
continent Asia
country Malaysia
content_provider Universiti Putra Malaysia
content_source UPM Institutional Repository
url_provider http://psasir.upm.edu.my/
language English
description Stevia rebaudiana is a unique plant that contains non-caloric natural sweetener and has gained much interest among Malaysians. In this study, the effect of different concentrations of N‑methyl-N-nitrosourea (MNU) was assessed in inducing mutation in Stevia seeds to produce genetic variations, which is valuable for crop improvement. Stevia seeds were soaked in six concentrations of MNU (0.0, 0.13, 0.25, 0.38, 0.50, and 1.00 mM) for four different durations (15, 30, 45, and 60 min) at room temperature. As a result, application of MNU reduced the germination percentage and germination rate of Stevia seeds as compared to the control group. Prolonged exposure to the highest concentration of MNU recorded the lowest percentage of germination (2.5 ± 1.4%) and the lowest germination rate (0.21 ± 0.16). Tricots were observed among seedlings treated with 0.13, 0.38 and 1.0 mM of MNU for 30 min. Presence of seedlings with albino colour proved the mutagenic effect of MNU on Stevia genome. Based on the percentage of seedlings with chlorophyll mutation, the most effective and efficient mutagenic treatment to induce mutation was 60 min in 0.25 mM of MNU.
format Article
author Ahmad, Lyena Watty Zuraine
Zainuddin, Zarina
Osman, Mohamad
Mohamad, Azhar
Jaafar Sidik, Norrizah
spellingShingle Ahmad, Lyena Watty Zuraine
Zainuddin, Zarina
Osman, Mohamad
Mohamad, Azhar
Jaafar Sidik, Norrizah
Effects of N-methyl-N-nitrosourea on seed germination of Stevia rebaudiana
author_facet Ahmad, Lyena Watty Zuraine
Zainuddin, Zarina
Osman, Mohamad
Mohamad, Azhar
Jaafar Sidik, Norrizah
author_sort Ahmad, Lyena Watty Zuraine
title Effects of N-methyl-N-nitrosourea on seed germination of Stevia rebaudiana
title_short Effects of N-methyl-N-nitrosourea on seed germination of Stevia rebaudiana
title_full Effects of N-methyl-N-nitrosourea on seed germination of Stevia rebaudiana
title_fullStr Effects of N-methyl-N-nitrosourea on seed germination of Stevia rebaudiana
title_full_unstemmed Effects of N-methyl-N-nitrosourea on seed germination of Stevia rebaudiana
title_sort effects of n-methyl-n-nitrosourea on seed germination of stevia rebaudiana
publisher Penerbit Universiti Kebangsaan Malaysia
publishDate 2019
url http://psasir.upm.edu.my/id/eprint/69286/1/Effects%20of%20N-methyl-N-nitrosourea%20on%20seed%20germination%20of%20Stevia%20rebaudiana.pdf
http://psasir.upm.edu.my/id/eprint/69286/
http://www.ukm.my/jsm/english_journals/vol48num2_2019/contentsVol48num2_2019.html
_version_ 1665895988084080640
score 13.211869

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