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Effects of lignosulfonates on callus proliferation and shoot induction of recalcitrant indica rice

In vitro culture of recalcitrant indica rice cultivar through intervening callus is difficult due to long regeneration period. Therefore, this study was undertaken to evaluate the growth promoting effects of lignosulfonate (LS) on callus proliferation and shoot induction of Malaysian recalcitrant in...

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Main Authors: Low, Lee Yoon, Abdullah, Janna Ong, Wee, Chien Yeong, Sekeli, Rogayah, Tan, Chun Keat, Loh, Jiun Yan, Lai, Kok Song
Format: Article
Language:English
Published: Penerbit Universiti Kebangsaan Malaysia 2019
Online Access:http://psasir.upm.edu.my/id/eprint/69284/1/Effects%20of%20lignosulfonates%20on%20callus%20proliferation%20and%20shoot%20induction%20of%20recalcitrant%20indica%20rice.pdf
http://psasir.upm.edu.my/id/eprint/69284/
http://www.ukm.my/jsm/english_journals/vol48num1_2019/contentsVol48num1_2019.html
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spelling my.upm.eprints.692842020-03-25T09:12:40Z http://psasir.upm.edu.my/id/eprint/69284/ Effects of lignosulfonates on callus proliferation and shoot induction of recalcitrant indica rice Low, Lee Yoon Abdullah, Janna Ong Wee, Chien Yeong Sekeli, Rogayah Tan, Chun Keat Loh, Jiun Yan Lai, Kok Song In vitro culture of recalcitrant indica rice cultivar through intervening callus is difficult due to long regeneration period. Therefore, this study was undertaken to evaluate the growth promoting effects of lignosulfonate (LS) on callus proliferation and shoot induction of Malaysian recalcitrant indica rice cv. MR219. LS is a by-product of wood industry, commonly used as a plant growth enhancer. Seed derived calli were proliferated on Murashige and Skoog (MS) medium supplemented with different ion-chelated LS (calcium LS: CaLS and sodium LS: NaLS) at 50, 100, 150, and 200 mg/L. MS supplemented with 100 mg/L CaLS significantly increased the callus proliferation rate and adventitious root formation. In shoot induction study, both LSs did not enhance the shoot induction efficiency as compared to the control. However, the formation of albino shoot increased in MS fortified with 100 mg/L CaLS. Further chlorophyll and molecular analyses showed that, albino shoots induced from 100 mg/L CaLS had severe reduction in total chlorophyll content and expression of both chlorophyll-associated genes, chlorophyll a/b-binding protein 1 (OsCAB1R) and young seedling albino (OsYSA). Taken together, LS improves callus proliferation rate and modulate different physiological responses during plant growth of recalcitrant indica rice. Penerbit Universiti Kebangsaan Malaysia 2019 Article PeerReviewed text en http://psasir.upm.edu.my/id/eprint/69284/1/Effects%20of%20lignosulfonates%20on%20callus%20proliferation%20and%20shoot%20induction%20of%20recalcitrant%20indica%20rice.pdf Low, Lee Yoon and Abdullah, Janna Ong and Wee, Chien Yeong and Sekeli, Rogayah and Tan, Chun Keat and Loh, Jiun Yan and Lai, Kok Song (2019) Effects of lignosulfonates on callus proliferation and shoot induction of recalcitrant indica rice. Sains Malaysiana, 48 (1). pp. 7-13. ISSN 0126-6039 http://www.ukm.my/jsm/english_journals/vol48num1_2019/contentsVol48num1_2019.html 10.17576/jsm-2019-4801-02
institution Universiti Putra Malaysia
building UPM Library
collection Institutional Repository
continent Asia
country Malaysia
content_provider Universiti Putra Malaysia
content_source UPM Institutional Repository
url_provider http://psasir.upm.edu.my/
language English
description In vitro culture of recalcitrant indica rice cultivar through intervening callus is difficult due to long regeneration period. Therefore, this study was undertaken to evaluate the growth promoting effects of lignosulfonate (LS) on callus proliferation and shoot induction of Malaysian recalcitrant indica rice cv. MR219. LS is a by-product of wood industry, commonly used as a plant growth enhancer. Seed derived calli were proliferated on Murashige and Skoog (MS) medium supplemented with different ion-chelated LS (calcium LS: CaLS and sodium LS: NaLS) at 50, 100, 150, and 200 mg/L. MS supplemented with 100 mg/L CaLS significantly increased the callus proliferation rate and adventitious root formation. In shoot induction study, both LSs did not enhance the shoot induction efficiency as compared to the control. However, the formation of albino shoot increased in MS fortified with 100 mg/L CaLS. Further chlorophyll and molecular analyses showed that, albino shoots induced from 100 mg/L CaLS had severe reduction in total chlorophyll content and expression of both chlorophyll-associated genes, chlorophyll a/b-binding protein 1 (OsCAB1R) and young seedling albino (OsYSA). Taken together, LS improves callus proliferation rate and modulate different physiological responses during plant growth of recalcitrant indica rice.
format Article
author Low, Lee Yoon
Abdullah, Janna Ong
Wee, Chien Yeong
Sekeli, Rogayah
Tan, Chun Keat
Loh, Jiun Yan
Lai, Kok Song
spellingShingle Low, Lee Yoon
Abdullah, Janna Ong
Wee, Chien Yeong
Sekeli, Rogayah
Tan, Chun Keat
Loh, Jiun Yan
Lai, Kok Song
Effects of lignosulfonates on callus proliferation and shoot induction of recalcitrant indica rice
author_facet Low, Lee Yoon
Abdullah, Janna Ong
Wee, Chien Yeong
Sekeli, Rogayah
Tan, Chun Keat
Loh, Jiun Yan
Lai, Kok Song
author_sort Low, Lee Yoon
title Effects of lignosulfonates on callus proliferation and shoot induction of recalcitrant indica rice
title_short Effects of lignosulfonates on callus proliferation and shoot induction of recalcitrant indica rice
title_full Effects of lignosulfonates on callus proliferation and shoot induction of recalcitrant indica rice
title_fullStr Effects of lignosulfonates on callus proliferation and shoot induction of recalcitrant indica rice
title_full_unstemmed Effects of lignosulfonates on callus proliferation and shoot induction of recalcitrant indica rice
title_sort effects of lignosulfonates on callus proliferation and shoot induction of recalcitrant indica rice
publisher Penerbit Universiti Kebangsaan Malaysia
publishDate 2019
url http://psasir.upm.edu.my/id/eprint/69284/1/Effects%20of%20lignosulfonates%20on%20callus%20proliferation%20and%20shoot%20induction%20of%20recalcitrant%20indica%20rice.pdf
http://psasir.upm.edu.my/id/eprint/69284/
http://www.ukm.my/jsm/english_journals/vol48num1_2019/contentsVol48num1_2019.html
_version_ 1665895987988660224
score 13.211869

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