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Total antioxidant activity and enzymatic inhibition against alpha-amylase, alpha-glucosidase and pancreatic lipase of irradiated Archidendron bubalinum

Introduction: Archidendron bubalinum is an underutilised plant with numerous antioxidant properties and has a great potential to inhibit enzymes linked with diabetes and obesity. Food irradiation is an advanced technology to prolong the lifespan of plant, prevent physical spoilage and eradicate food...

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Main Authors: Ja'afar, Nor Liyana, Chai, Pui Teng, Mohd Rosmi, Noor Syafiqa Aqila, Rambli, Siti Nur Atiqah, Shafie, Nurul Husna, Arapoc, Daryl Jesus, Bahari, Hasnah
Format: Article
Language:English
Published: Faculty of Medicine and Health Sciences, Universiti Putra Malaysia 2019
Online Access:http://psasir.upm.edu.my/id/eprint/68401/1/2019041008272217_MJMHS_Vol_15_SP1.pdf
http://psasir.upm.edu.my/id/eprint/68401/
http://www.medic.upm.edu.my/upload/dokumen/2019041008272217_MJMHS_Vol_15_SP1.pdf
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spelling my.upm.eprints.684012019-05-10T08:34:36Z http://psasir.upm.edu.my/id/eprint/68401/ Total antioxidant activity and enzymatic inhibition against alpha-amylase, alpha-glucosidase and pancreatic lipase of irradiated Archidendron bubalinum Ja'afar, Nor Liyana Chai, Pui Teng Mohd Rosmi, Noor Syafiqa Aqila Rambli, Siti Nur Atiqah Shafie, Nurul Husna Arapoc, Daryl Jesus Bahari, Hasnah Introduction: Archidendron bubalinum is an underutilised plant with numerous antioxidant properties and has a great potential to inhibit enzymes linked with diabetes and obesity. Food irradiation is an advanced technology to prolong the lifespan of plant, prevent physical spoilage and eradicate food borne disease. Present study was aimed to determine the total antioxidant activity, enzymatic inhibition against alpha-amylase, alpha-glucosidase and pancreatic lipase and the toxicity levels of non-irradiated and irradiated (3, 6, 9 & 12 kGy) hot aqueous extract of A. bubalinum. Methods: The antioxidant ability of the extract was determined by total phenolic content (TPC), total flavanoid content (TFC), Diphenyl-1-Picrylhydrazyl (DPPH), β-carotene assay and ferric reducing antioxidant potential (FRAP) assay. The inhibitory activities were evaluated using α-amylase, α-glucosidase, and pancreatic lipase inhibition as-say. The toxicity levels of A. bubalinum extract were determined using Brine shrimp and Zebra-fish assays. Results: Results showed that irradiated A. bubalinum at 12 kGy demonstrated the highest TFC (448.99 ± 5.02 mg GAE/g), FRAP (2.55 ± 0.40 mmol Fe2+/g) and β-carotene bleaching activity (79.49%). Whereas, non-irradiated A. bubalinum samples expressed the highest TPC (2517.07 ± 15.81 mg GAE/g) and exhibited the lowest IC50 values of α-amylase (31.99 ± 3.15 μg/ml), α-glucosidase (23.40 ± 0.69μg/ml) and pancreatic lipase (32.81 ± 7.96 μg/ml) activity. The toxicity assays also showed no significant different between irradiated and non-irradiated samples. Conclusion: The study suggests that gamma irradiation has the prospective future to increase antioxidant properties and maintaining the enzyme inhibitory activities to preserve the sample of A. bubalinum for commercial purposes. Faculty of Medicine and Health Sciences, Universiti Putra Malaysia 2019 Article PeerReviewed text en http://psasir.upm.edu.my/id/eprint/68401/1/2019041008272217_MJMHS_Vol_15_SP1.pdf Ja'afar, Nor Liyana and Chai, Pui Teng and Mohd Rosmi, Noor Syafiqa Aqila and Rambli, Siti Nur Atiqah and Shafie, Nurul Husna and Arapoc, Daryl Jesus and Bahari, Hasnah (2019) Total antioxidant activity and enzymatic inhibition against alpha-amylase, alpha-glucosidase and pancreatic lipase of irradiated Archidendron bubalinum. Malaysian Journal of Medicine and Health Sciences, 15 (SP1). pp. 120-128. ISSN 1675-8544; ESSN: 2636-9346 http://www.medic.upm.edu.my/upload/dokumen/2019041008272217_MJMHS_Vol_15_SP1.pdf
institution Universiti Putra Malaysia
building UPM Library
collection Institutional Repository
continent Asia
country Malaysia
content_provider Universiti Putra Malaysia
content_source UPM Institutional Repository
url_provider http://psasir.upm.edu.my/
language English
description Introduction: Archidendron bubalinum is an underutilised plant with numerous antioxidant properties and has a great potential to inhibit enzymes linked with diabetes and obesity. Food irradiation is an advanced technology to prolong the lifespan of plant, prevent physical spoilage and eradicate food borne disease. Present study was aimed to determine the total antioxidant activity, enzymatic inhibition against alpha-amylase, alpha-glucosidase and pancreatic lipase and the toxicity levels of non-irradiated and irradiated (3, 6, 9 & 12 kGy) hot aqueous extract of A. bubalinum. Methods: The antioxidant ability of the extract was determined by total phenolic content (TPC), total flavanoid content (TFC), Diphenyl-1-Picrylhydrazyl (DPPH), β-carotene assay and ferric reducing antioxidant potential (FRAP) assay. The inhibitory activities were evaluated using α-amylase, α-glucosidase, and pancreatic lipase inhibition as-say. The toxicity levels of A. bubalinum extract were determined using Brine shrimp and Zebra-fish assays. Results: Results showed that irradiated A. bubalinum at 12 kGy demonstrated the highest TFC (448.99 ± 5.02 mg GAE/g), FRAP (2.55 ± 0.40 mmol Fe2+/g) and β-carotene bleaching activity (79.49%). Whereas, non-irradiated A. bubalinum samples expressed the highest TPC (2517.07 ± 15.81 mg GAE/g) and exhibited the lowest IC50 values of α-amylase (31.99 ± 3.15 μg/ml), α-glucosidase (23.40 ± 0.69μg/ml) and pancreatic lipase (32.81 ± 7.96 μg/ml) activity. The toxicity assays also showed no significant different between irradiated and non-irradiated samples. Conclusion: The study suggests that gamma irradiation has the prospective future to increase antioxidant properties and maintaining the enzyme inhibitory activities to preserve the sample of A. bubalinum for commercial purposes.
format Article
author Ja'afar, Nor Liyana
Chai, Pui Teng
Mohd Rosmi, Noor Syafiqa Aqila
Rambli, Siti Nur Atiqah
Shafie, Nurul Husna
Arapoc, Daryl Jesus
Bahari, Hasnah
spellingShingle Ja'afar, Nor Liyana
Chai, Pui Teng
Mohd Rosmi, Noor Syafiqa Aqila
Rambli, Siti Nur Atiqah
Shafie, Nurul Husna
Arapoc, Daryl Jesus
Bahari, Hasnah
Total antioxidant activity and enzymatic inhibition against alpha-amylase, alpha-glucosidase and pancreatic lipase of irradiated Archidendron bubalinum
author_facet Ja'afar, Nor Liyana
Chai, Pui Teng
Mohd Rosmi, Noor Syafiqa Aqila
Rambli, Siti Nur Atiqah
Shafie, Nurul Husna
Arapoc, Daryl Jesus
Bahari, Hasnah
author_sort Ja'afar, Nor Liyana
title Total antioxidant activity and enzymatic inhibition against alpha-amylase, alpha-glucosidase and pancreatic lipase of irradiated Archidendron bubalinum
title_short Total antioxidant activity and enzymatic inhibition against alpha-amylase, alpha-glucosidase and pancreatic lipase of irradiated Archidendron bubalinum
title_full Total antioxidant activity and enzymatic inhibition against alpha-amylase, alpha-glucosidase and pancreatic lipase of irradiated Archidendron bubalinum
title_fullStr Total antioxidant activity and enzymatic inhibition against alpha-amylase, alpha-glucosidase and pancreatic lipase of irradiated Archidendron bubalinum
title_full_unstemmed Total antioxidant activity and enzymatic inhibition against alpha-amylase, alpha-glucosidase and pancreatic lipase of irradiated Archidendron bubalinum
title_sort total antioxidant activity and enzymatic inhibition against alpha-amylase, alpha-glucosidase and pancreatic lipase of irradiated archidendron bubalinum
publisher Faculty of Medicine and Health Sciences, Universiti Putra Malaysia
publishDate 2019
url http://psasir.upm.edu.my/id/eprint/68401/1/2019041008272217_MJMHS_Vol_15_SP1.pdf
http://psasir.upm.edu.my/id/eprint/68401/
http://www.medic.upm.edu.my/upload/dokumen/2019041008272217_MJMHS_Vol_15_SP1.pdf
_version_ 1643839190743384064
score 13.211869

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